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1.
BACKGROUND: Isothiocyanates (ITCs) released by the enzymatic hydrolysis of glucosinolates in the Brassicaceae are potentially useful for controlling fungal pathogens. In vitro activity of pure ITCs against Sclerotinia sclerotiorum (Lib.) de Bary was studied by adding them to glass filters in petri dishes and dissolving them in the growing media. RESULTS: Methyl, allyl and benzyl ITCs were the most fungitoxic of the compounds in bioassays with S. sclerotiorum isolate Ss31. In the volatile phase, mycelial growth was completely inhibited by these three compounds. Aromatic ITCs were less toxic in the petri dishes but were more toxic than aliphatic ITCs when dissolved in the agar. Benzyl ITC exhibited the highest inhibitory effect on sclerotial germination, with an EC50 value of 75.1 µmol L?1. Butyl and benzyl ITCs reduced apothecial production of S. sclerotiorum by 92.5% at the highest concentration. In in vivo assay, only allyl and 2‐phenylethyl ITCs reduced disease incidence (by 76.7 and 70% respectively) at low concentrations. CONCLUSION: Sclerotinia sclerotiorum in the soil might be suppressed by the higher concentrations of allyl and benzyl ITCs released from decomposition of Brassica juncea, B. carinata, B. nigra and Sinapis spp. Copyright © 2011 Society of Chemical Industry 相似文献
2.
Burak Kaptaner Güler Ünal Emine Doğan Handan Aykut 《Anatomia, histologia, embryologia》2021,50(2):404-410
In the present study, the location, histology and number of corpuscles of Stannius (Sc), which are endocrine glands associated with the kidneys of teleost fish, were investigated for the first time in Lake Van fish (Alburnus tarichi), an anadromous and endemic inhabiting Turkey's Lake Van Basin. The Sc, which were ovoid or spheroid and white or cream in colour, were found to vary in number between three and five among the examined fish. The glands were located in the caudal part of the kidney, and either partially or completely embedded, and found to be present on both the ventral and dorsal surface of either side of the caudal part of the kidney. The Sc were surrounded by a connective tissue capsule that penetrated and divided the gland into incomplete lobules. Two types of cells were determined in the parenchyma of the gland. Type-I cells were predominant throughout the parenchyma and larger than the second (type-II). In the type-I cells, the cytoplasm was observed as weakly or moderately eosinophilic with haematoxylin and eosin staining and weakly or moderately acidophilic with Mallory's triple staining. In the type-I cells, the cytoplasm exhibited weak to moderate periodic acid-Schiff staining and slight or uniform staining with aldehyde fuchsin. The type-II cells were round, had a darkly stained spherical nucleus and were dispersed among the type-I cells. They displayed no cytoplasmic staining with the abovementioned stains. 相似文献
3.
Fidan I Yesilyurt E Gurelik FC Erdal B Imir T 《Comparative immunology, microbiology and infectious diseases》2008,31(6):467-475
Salmonella typhi (S. typhi) is an important pathogen which causes typhoid fever. The cytokines released from the macrophages, playing a role in the host defense against Salmonella infection, are crucial in the defense against the infection. IFN-γ provides a protection against Salmonella infection by developing macrophage activation in different mechanisms. This study was designed to investigate the effect of the recombinant IFN-γ (rIFN-γ) on the cytokines secreted from S. typhi stimulated macrophages. Macrophage isolation was done in the heparinized blood samples obtained from healthy people, and following the priming with rIFN-γ for 72 h the cells were stimulated by S. typhi and then the cytokine levels in culture supernatants were determined by enzyme-linked immunosorbent assay. It was observed that rIFN-γ reversely increased the levels of IL-1, IL-2 the levels of which were decreased by S. typhi and that it increased TNF-α levels while suppressing the levels of antiinflammatory cytokines such as IL-10 and TGF-β the levels of which were increased by S. typhi. Consequently, rIFN was observed to increase protective Th1 response by affecting the secretion of cytokine during S. typhi infection and it was considered to be a good target especially to prevent and treat invasive Salmonella infections. 相似文献
4.
Echinochloa crus-galli (L.) P. Beauv. (barnyardgrass) is an annual weed that is native to Asia and found throughout the world. The broad ecological
tolerance and competitive ability of E. crus-galli makes it the most important weed species in rice. Genetic studies of plants are becoming increasingly common because reliable
information is necessary to better understand population dynamics, occurrence of herbicide resistance, and demographic data.
Echinochloa crus-galli populations from 34 different locations in Turkey were compared with respect to morphological differences and genetic variation.
For morphological variation, five seeds of each population were sown in pots and grown in a screenhouse using a randomized
block design. Morphological parameters such as germination speed, flowering time, leaf area, plant height, spikelet length,
above-ground biomass, root dry weight and number of seeds were measured. Distinct differences among populations with respect
to hierarchical cluster analysis were observed. Genetic variations among populations were performed using random amplified
polymorphic DNA (RAPD) markers. The seven RAPD primers amplified 55 bands whose molecular weight varied between 200 and 4000 bp.
The percentage of polymorphic bands was 74.54%. Results showed high morphological and genetic variability among individual
genotypes within geographic locations. Phenotypic and genetic variability among E. crus-galli populations would be influenced by agricultural practices, crop characteristics, geographic location and herbicide pressure.
Differences between weed populations may affect response to chemical or biological control. 相似文献
5.
In a study carried out during 2002 and 2004 in Diyarbakıir and Mardin provinces, southeast Turkey, specimens were collected
twice a month from cultivated and non-cultivated plants. Sixteen leafminer (Diptera: Agromyzidae) species were identified.
Among them,Agromyza abiens Zetterstedt, 1848;Napomyza elegans (Meigen, 1830);Phytoliriomyza dorsata (Siebke, 1864); andPhytomyza aquilonia Frey, 1964 are new records for the Turkish leafminer
http://www.phytoparasitica.org posting July 24, 2005. 相似文献
6.
Journal of Plant Diseases and Protection - The two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), is one of the most serious pests of many crops both indoors and outdoors in... 相似文献
7.
The objective of this study was to evaluate the potential to increase the level of resistant starch (RS) in extruded products by optimizing extruder conditions. Three experiments were conducted as randomized complete block designs with two replicates. In the first experiment, corn starch, wheat starch, and potato starch were added at a level of 30% (w/w) to degerminated yellow corn meal to investigate the influence of starch type. In the second experiment, citric acid (CA) monohydrate was added to corn meal at levels of 0, 2.5, 5, and 7.5% (w/w). The third experiment was a full-factorial arrangement to evaluate the effect of high-amylose corn starch (HACS) level (0, 15, 30%, w/w) and CA level (0, 5, 7.5%, w/w) at two screw speeds (200 and 300 rpm). In the first experiment, the means for RS plus dietary fiber for the different starch formulations ranged from 1.27 to 2.28%. In experiment 2, adding CA increased RS plus dietary fiber content to a maximum of 5.23% at 7.5% CA. In the third experiment, the means for RS plus dietary fiber ranged from a low of 1.75% for 100% corn meal at 300 rpm to 14.38% for 7.5% CA and 30% HACS at 200 rpm. The results indicated a highly significant positive relationship between CA and RS formation and the same for amylose content. The RS formation had a negative relationship with screw speed, but the influence of screw speed was small when compared with that of CA and HACS. 相似文献
8.
The dynamics of inorganic N in soil following the application of plant residues depends on their composition. We assumed that all plant materials are composed of similar components, each decomposing at a specific rate, but differ in the proportions of the various components. The NCSOIL model that simulates C and N turnover in soil was used to link the rates of residue decomposition to their composition, defined as soluble, cellulose-like and lignin-like C and N, and thereby integrate short and long-term effects of residues on available N dynamics in soil. Five plant residues in a wide range of C:N ratios were incubated in soil for 24 weeks at 30 °C, during which C and N mineralization were measured. The materials with large C:N ratios (corn, rice hulls and wheat straw) were also incubated with NH4+-N to avoid N deficiency. The residues were analyzed for total and soluble C and N. The partitioning of insoluble C and N between cellulose- and lignin-like pools was optimized by best fit of simulated C and N mineralization to measured results. The decomposition rate constants of the soluble and lignin-like pools were assumed to be 1.0 and 10−5 d−1, respectively, and that of the cellulose-like pool, obtained by model optimization against mineralization of cellulose with NH4+-N in soil, was 0.051 d−1. The optimized, kinetically defined lignin-like pool of all residues was considerably larger than lignin contents normally found in plant residues by the Van Soest procedure. Gross N mineralization of tobacco and rape residues was similar, but N recovery from tobacco was larger, because a larger fraction of its C was in the lignin-like pool. N in rice hulls, corn and wheat residues was mostly recalcitrant, yet rice hulls did not cause N deficiency, because most of its C was recalcitrant too. The soluble components of the residues had strong short-term effects on available N in soil, but the cellulose-like pool was equally important for short and medium-term effects. Soluble and cellulose-like C were 29 and 42% of total C, respectively, in corn and 7 and 50% in wheat. Maximal net inorganic N losses, measured in both residue treatments after 2 weeks, were 42 mg g−1 C applied as corn and 31 mg g−1 C applied as wheat, or 84 and 110 mg g−1 decomposed C of corn and wheat, respectively. Rice hulls immobilized N slowly, but by the end of 24 weeks all three residues immobilized 26-27 mg N kg−1 C applied. The different dynamics of N immobilization demonstrated the need to determine the decomposability of C and N rather than their total contents in plant residues. 相似文献
9.
Ayşe Gürel Inanlı Özlem Emir Çoban Ökkeş Yılmaz Emine Özpolat Nermin Karaton Kuzgun 《Journal Of Aquatic Food Product Technology》2018,27(7):803-810
In this study, roes obtained from carp (Cyprinus carpio carpio) that were caught in Keban Dam Lake (Turkey) and cultured rainbow trout (Oncorhynchus mykiss) were processed as caviar, and the products were stored at + 4 C°. Lipid peroxidation and the compositions of fatty acids of these products were assessed during production and storage period. The result of fatty analyses showed that palmitic acid (16:0), stearic acid (18:0), palmitoleic acid (16:1), oleic acid (18:1), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), which are all essential fatty acids, were determined in the caviars of the roes obtained from rainbow trout and carp. According to results of chemical analyses, there were no significant differences in thiobarbituric acid reactive substances (TBARs) between rainbow trout and carp caviars (p > 0.05), but malondialdehyde (MDA) levels were found to be statistically significant (p < 0.01). It can be concluded that caviars obtained from both rainbow trout and carp roes are rich in fatty acids and take a part in a balanced and healthy diet. 相似文献
10.
Tevfik Ozen Hamdi Zenginbal Emine Yazicioglu Fatih Gul Ibrahim Demirtas 《International Journal of Fruit Science》2019,19(2):115-135
The present work was conducted to find the physicochemical properties, chemical components and antioxidant capacity of kiwifruit hydroalcoholic extracts (B-31, HO-1-14, J-242, Bruno, Greenlight, Hayward, Monty, Topstar) grown Yalova, Turkey. Phytochemical tests were performed to determine total phenolic, flavonoid and ascorbic acid. Moreover, the kiwifruit extracts were subjected to evaluate their antioxidant potential using different in vitro assays such as total antioxidant, inhibition of lipid peroxidation, reducing power, metal chelating, free radical, H2O2 and superoxide anion scavenging activities at 50–500 μg mL?1, and also found EC50 and IC50 values. The soluble solid content (SSC), titratable acidity (TA) and pH were analyzed in all the genotypes and cultivars. The highest total phenolic, flavonoid and ascorbic acid content were found in J-242 (89.53 mg GAE/100 g), J-242 (109.13 mg QE/100 g) and HO-1-14 (64.15 mg/100 g), respectively. The linoleic, palmitic, stearic and oleic acid methyl esters were detected in all kiwifruit extracts by GC/MS. Significant differences were found between genotypes and cultivars in the antioxidant tests. The extracts showed high inhibition of lipid peroxidation and compared to standards, significantly (p < 0.05). The GC/MS analyses confirmed quantitative variability in the antioxidant profiles. The SSC, TA and pH of kiwifruit genotypes and cultivars showed a moderate level. The key parameters for the most active kiwifruit included the following: total antioxidant activity for B-31, H2O2 scavenging activity for J-242 and metal chelating activity for Topstar. In conclusion, this study exhibited the important role played by the genotypes and cultivars background on the chemical content and antioxidant profiles. 相似文献