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Survey on whiteflies and their parasitoids in cassava mosaic pandemic areas of Tanzania using morphological and molecular techniques 下载免费PDF全文
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D. Marletta S. Bordonaro A. M. Guastella G. D'Urso 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2004,121(1):52-56
In this study we surveyed two endangered Sicilian goat breeds (Girgentana and Argentata dell'Etna) for genetic polymorphism at the CSN1S2 locus. In a total of 537 goats, we detected CSN1S2A, CSN1S2B, CSN1S2C° (CSN1S2C + CSN1S2E), CSN1S2D, CSN1S2F and CSN1S2O alleles by means of various polymerase chain reaction (PCR), allele specific‐PCR (AS–PCR) and PCR‐restriction fragment length polymorphism (RFLP) reactions with the aim of improving the knowledge of the genetic resource of these two breeds. Three and five alleles, with six and twelve genotypes, were identified at CSN1S2 locus, in Girgentana and Argentata, respectively. Argentata dell'Etna showed a higher degree of genetic variation. The allelic and genotypic distribution seems to be significantly different (p < 0.001) in the two breeds. In Argentata the rare null allele (CSN1S2O) was found at low frequency (0.033); this genetic peculiarity makes its preservation worthwhile. 相似文献
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Steven R. James Lori Wing Ronald Knight Barbara Harrity Devid H. Lambert Bud Platt Al Reeves Bill Bohl Lind Sanford Alexander D. Pavlista Ed Plissey Richard Sawyer Joe Guenthner Willem Schrage Edward B. Radcilffe Nora Olsen Jeff Miller Rikki Sterrett Gale W. Harding Marcin Topoleweki 《American Journal of Potato Research》2000,77(6):369-390
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Cloning and expression of a rat brain GABA transporter 总被引:45,自引:0,他引:45
J Guastella N Nelson H Nelson L Czyzyk S Keynan M C Miedel N Davidson H A Lester B I Kanner 《Science (New York, N.Y.)》1990,249(4974):1303-1306
A complementary DNA clone (designated GAT-1) encoding a transporter for the neurotransmitter gamma-aminobutyric acid (GABA) has been isolated from rat brain, and its functional properties have been examined in Xenopus oocytes. Oocytes injected with GAT-1 synthetic messenger RNA accumulated [3H]GABA to levels above control values. The transporter encoded by GAT-1 has a high affinity for GABA, is sodium-and chloride-dependent, and is pharmacologically similar to neuronal GABA transporters. The GAT-1 protein shares antigenic determinants with a native rat brain GABA transporter. The nucleotide sequence of GAT-1 predicts a protein of 599 amino acids with a molecular weight of 67 kilodaltons. Hydropathy analysis of the deduced protein suggests multiple transmembrane regions, a feature shared by several cloned transporters; however, database searches indicate that GAT-1 is not homologous to any previously identified proteins. Therefore, GAT-1 appears to be a member of a previously uncharacterized family of transport molecules. 相似文献
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Analysis of genetic diversity and the determination of relationships among western Mediterranean horse breeds using microsatellite markers 总被引:2,自引:0,他引:2
D. Marletta I. Tupac-Yupanqui S. Bordonaro D. García A. M. Guastella A. Criscione J. Cañón & S. Dunner 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2006,123(5):315-325
The distribution of genetic diversity and the genetic relationships among western Mediterranean horse breeds were investigated using microsatellite markers. The examined sample included seven Spanish and three Italian local horse breeds and populations, plus a Spanish Thoroughbred outgroup. The total number of animals examined was 682 (on average 62 animals per breed; range 20–122). The microsatellite marker set analysed provided 128 alleles (10.7 alleles per locus). Within‐breed genetic diversity was always high (>0.70), with breeds contributing about 8% of the total genetic variability. The mean molecular coancestry of the entire population examined was 0.205, Losino being the breed that contributed most. In addition to Nei's standard and Reynolds’ genetic distances, pair‐wise kinship distance and molecular coancestry were estimated. Remarkably similar breed rankings were obtained with all methods. Clustering analysis provided an accurate representation of the current genetic relationships among the breeds. Determining coancestry is useful for analysing genetic diversity distribution between and within breeds and provides a good framework for jointly analysing molecular markers and pedigree information. An integrated analysis was undertaken to obtain information on the population dynamics in western Mediterranean native horse populations, and to better determine conservation priorities. 相似文献
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D B Guastella D B Fox J L Cook 《Veterinary and comparative orthopaedics and traumatology》2008,21(2):125-128
OBJECTIVES: To compare tibial plateau angle (TPA) among Labrador Retrievers, Rottweilers, Boxers, and German Shepherd Dogs that had undergone tibial plateau leveling osteotomies (TPLO) for cranial cruciate ligament disease, and to determine if higher TPAs are associated with increased risk for concurrent meniscal injury. METHODS: This was a retrospective study including 275 client-owned dogs of one of the four aforementioned breeds that received TPLO radiographs between 1999 and 2005 prior to undergoing the TPLO procedure. The TPA measured by the original surgeon was compared among four breeds and analyzed with respect to presence or absence of meniscal injury diagnosed via arthroscopy or arthrotomy at the time of surgery. RESULTS: Labrador Retrievers, Rottweilers, Boxers, and German Shepherd Dogs had mean TPAs of 25.9 degrees , 26.2 degrees , 25.9 degrees , and 28.2 degrees , respectively. The TPA of German Shepherd Dogs was significantly higher than that of the other breeds of dogs examined. TPA higher than the mean for dogs in this study did not significantly increase the risk for concurrent meniscal injury. CLINICAL SIGNIFICANCE: German Shepherd Dogs with cranial cruciate ligament disease had a significantly higher TPA compared to the other breeds examined. Higher TPA did not increase the likelihood of meniscal tears in this study. However, several variables that were not assessed, including the degree of cranial cruciate ligament integrity at the time of surgery and the time between original CrCL deficiency and surgical assessment, could have affected this analysis. 相似文献
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Genetic relationships among American donkey populations: insights into the process of colonization 下载免费PDF全文
J. Miró F. Goyache A. Loarca O.R. Martínez López J.L. Canelón A. Stemmer L. Aguirre M.A.C. Lara L.A. Álvarez S. Llambí N. Gómez L.T. Gama M.F. Nóvoa R.D. Martínez E. Pérez A. Sierra M.A. Contreras A.M. Guastella D. Marletta G. Arsenos I. Curik V. Landi A. Martínez J.V. Delgado 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2016,133(2):155-164
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