河南省烟草病毒的小RNA测序检测

To identify viruses in Henan tobacco-planting areas, from 2015 to 2017 and 2019, 288 symptomatic tobacco samples were collected and then subjected to small RNA sequencing. Results showed that at least 7 viruses were detected from these samples which including four previously reported viruses, cucumber mosaic virus (CMV), tobacco mosaic virus (TMV), potato virus Y (PVY) and tobacco vein banding mosaic virus (TVBMV). Other three viruses, wild tomato mosaic virus (WTMV), brassica yellows virus (BrYV), and cycas necrotic stunt virus (CNSV) were firstly detected in Henan province. However, tobacco etch virus (TEV) and tobacco ringspot virus (TRSV) were not detected from these samples. In addition, CMV, TMV, PVY, TVBMV, and BrYV were the dominant viruses infecting tobacco in Henan Province.     

侵染月季的香石竹潜隐病毒属新病毒鉴定及其全基因组序列分析

 2019年7月,在北京市月季叶片上观察到了黄色斑驳症状,高通量测序(High-throughput sequencing,HTS)显示在感病样本中存在一种新的正义ssRNA病毒。该病毒全基因组序列除去3′末端poly(A)后由8 393个核苷酸(nucleotide,nt)组成,含5个开放阅读框(open reading frame,ORF)。其依赖于RNA的RNA聚合酶(RNA-dependent RNA polymerase,RdRp)在氨基酸水平上与已知乙型线状病毒科(Betaflexiviridae)成员具有35.87% ~ 46.74%的序列一致性。系统发育分析表明,该新病毒与香石竹潜隐病毒属(Carlavirus)的杨树花叶病毒(poplar mosaic virus,PopMV)亲缘关系最近。发生率调查显示该新病毒在北京地区分布较为广泛。根据Betaflexiviridae的划分标准,该病毒是Carlavirus的一个新种,暂时命名为月季C病毒(rose virus C)。     

北京月季病原病毒的高通量测序鉴定和RT-PCR检测

本研究利用高通量测序技术对北京地区的月季染病样品进行了病毒鉴定,通过序列比对和拼接获得了李属坏死环斑病毒(prunus necrotic ringspot virus,PNRSV)、苹果茎沟病毒(apple stem grooving virus,ASGV)、柑橘碎叶病毒(citrus tatter leaf viru...