甘肃连城自然保护区的生态旅游资源及其评价

位于兰州市西北部的甘肃连城自然保护区,是一个具备丰厚的自然及人文旅游资源的国家级自然保护区.经多方位多层次的调查,在获取该保护区生态旅游资源完备材料的前提下,对其生态旅游资源作了分类描述;在作单项评价后,运用层次分析法对连城自然保护区的生态旅游资源进行了综合评价,据此提出了开发建议.     

桃果实蒸腾强度对源叶净光合效率及相关生理反应的影响

在果核硬化期和果实最后迅速生长期,对艳丰一号桃果实浸涂10倍和100倍的液体石蜡溶液,与果实未浸涂液体石蜡的对照相比,果实蒸腾强度分别降低了57.9%和41.4%,且源叶净光合效率(Pn)和气孔导度(Gs)降低而叶表面温度(Tl)升高,并在果实迅速生长期间,果实浸涂液体石蜡处理和对照之间存在显著性差异。进一步研究表明,无论是在果核硬化期还是在果实最后迅速生长期,当Gs小于0.2mol/m2·s时,Pn和Gs呈极显著直线正相关关系,且果实浸涂液体石蜡处理的源叶的Tl随Gs减小急剧升高。源叶Pn对Tl的响应均呈极显著抛物线相关关系,但同一Tl条件下,果实浸涂液体石蜡处理的Pn低于对照。因此认为,果实蒸腾强度可能通过作用叶片的Gs调节Tl进而对源叶的Pn进行调控,气孔开张度减小、Tl升高,可能是果实蒸腾强度减弱时调控Pn重要机制之一。     

篱壁式红富士苹果光照分布及结果部位对果实品质的影响

作者对6～7年生篱壁式红富士苹果叶幕光照分布、结果部位对品质的影响进行了研究.结果表明:篱壁形叶幕光照垂直分布和水平分布的日变化相对均衡,树冠内未产生无效光区;品质垂直和水平分布与光照垂直和水平分布呈极显著正相关.中、下层中长果枝果实的果面光洁度分别为92.7%和97.2%,端正果率分别为91.6%和92.5%.     

Analysis of the distribution and clonality of TCR Vβ T cells in cord blood

AIM:To investigate the distribution and clonality of TCR Vβ subfamily T cells in cord blood. METHODS:The CDR3 of TCR Vβ 24 subfamily genes were amplified in mononuclear cells from 13 cases of cord blood. To observe the usage of TCR Vβ repertoire, the PCR products were further labeled with fluorescent and analyzed by genescan technique for the CDR3 size, to evaluate clonality of the detectable TCR Vβ T cells. Peripheral bloods from 10 cases of normal individuals and T cell line Molt-4 and Jurkat served as controls. RESULTS:Only 38.78%±16.26% of 24 Vβ subfamily T cell were selectively expressed in cord blood, predominantly in Vβ 3, 5, 8, 9 and 13, whereas all 24 Vβ subfamilies could be detected in T cells from peripheral blood of normal individuals. Genescan analysis showed that all PCR products of TCR Vβ subfamilies from cord blood or normal individual peripheral blood displayed multi-peaks. CONCLUSION:Some TCR Vβ subfamily T cells were absent in cord blood. All TCR Vβ subfamily T cells in cord blood displayed polyclonality.     

Adult rat and human bone marrow mesenchymal stem cells differentiate into neurons with Musk's polypeptide

AIM: To investigate the differentiation from adult rat and human bone marrow mesenchymal stem cells (BMMSCs) into neuron with musk polypeptide (Mu-P).METHODS: Adult rat and human BMMSCs were induced with Mu-P.Neuron-specific enolase (NSE),neurofilament (NF),Nestin,glial fibrillary acidic protein (GFAP) were detected by immunohistochemistry.RESULTS: Simple methods with Mu-P induced adult rat and human BMMSCs exhibiting a neuronal phenotype,expressing Nestin at 3 hours to 5 hours,and expressing NE and NF at 5 hours to 7 days.But the neuron-like cells didn't express the glial astrocyte marker GFAP.CONCLUSION: Adult rat and human BMMSCs can be induced to differentiate into neurons with Mu-P.     

Expression,Purification and Activity Evaluation of Mb1230 Gene of Mycobacterium bovis

To explore the value of Mb1230 protein of Mycobacterium bovis in the diagnosis of bovine tuberculosis,we obtained the Mb1230 gene by PCR and constructed recombinant plasmid pET-22b-Mb1230.Recombinant Mb1230 protein was obtained by IPTG induction and purified by affinity chromatography.The activity of the recombinant protein was evaluated by TST test,IGRA test and indirect ELISA.The size of the recombinant protein matched with the theoretical value proved by SDS-PAGE;Western blotting result showed that the recombinant protein could react with mouse anti-His antibody,and had specific band;The results of TST test,IGRA test and indirect ELISA test also showed the recombinant protein had antigenic activity.The results indicated the recombinant protein Mb1230 had good B cell and T cell activity,so,it had the potential application in the diagnosis of bovine tuberculosis.     

新型城镇化建设背景下农村人力资源系统的研究与开发

新型城镇化建设背景下农村人力资源的开发愈发重要。阐述了农村人力资源管理信息系统的涵义,剖析了农村人力资源开发与管理的信息化应用现状及存在问题,挖掘了农村人力资源信息系统的应用层面,提出了农村人力资源系统开发应坚持的原则。     

葡萄汁奶酒发酵工艺研究

本文以牛奶和葡萄为原料,通过单因素和正交试验优化了葡萄汁奶酒的发酵工艺。结果表明,影响葡萄汁奶酒感官评分因素的强弱顺序依次为发酵温度>接种量>发酵时间>蔗糖添加量>葡萄汁添加量,通过计算发现最佳组合为发酵温度25℃,发酵时间5d,接种量1.00%∶0.50%∶1.50%,蔗糖添加量8%,葡萄汁添加量11%。该组合酿制的葡萄汁奶酒感官评分最高,为92分。经测得,葡萄汁奶酒在最佳发酵工艺条件下的总糖为9.5g/L、酒精体积分数为6.8%,总酸质量浓度为7.2g/L,氨基酸态氮质量浓度为0.21g/L。该葡萄汁奶酒中的天门冬氨酸、谷氨酸、异亮氨酸、亮氨酸、赖氨酸和脯氨酸含量显著高于奶酒和牛奶中的含量(P<0.05)。     

制约奶业发展的四大瓶颈和出路探讨

文章从分析三聚氰胺事件发生的原因入手，分析了制约中国奶业发展的四大结症，并提出了中国奶业持续、健康发展的必由之路。     

牛二酰甘油转酰基酶(DGAT1)基因的生物信息学分析

DGAT1基因作为影响奶牛产奶和肉牛肉质性状的主效基因之一,引起了人们越来越多的关注。本文应用生物信息学方法比较了人、牛、猪、绵羊等24个物种的DGAT1基因编码区(CDS)核苷酸和蛋白质序列,并对该基因的遗传多样性进行了分析。结果表明,DGAT1基因的CDS核苷酸和蛋白质序列长度多样性丰富;具有终止密码子偏爱性,包括牛在内的所有脊椎动物终止密码子都为TGA;在长度为1 187bp的33条基因序列中检测到992个多态位点,共生成26种单倍型。从聚类分析图可以看出,脊椎动物、无脊椎动物、植物和微生物的DGAT1基因之间存在着进化分歧,但界内差异较小。