Effect of landscape structure on anopheline mosquito density and diversity in northern Thailand: Implications for malaria transmission and control

The influence of landscape structure on anopheline mosquito density and diversity was studied in a comparison of agricultural and forested landscapes in northern Thailand. Agriculture locations had significantly higher landscape diversity, more patches, smaller mean patch sizes, and more complex patch shapes than forest locations. Mosquito collections were undertaken during both dry and wet seasons from October 1997 to December 1999. The density of two forest-associated species, Anopheles maculatus s.s. and Anopheles minimus s.l., both primary malaria vectors in Thailand, was significantly higher in forest locations in at least one season. The density of two paddy field-associated species, Anopheles aconitus and Anopheles hyrcanus group did not differ between locations. Anopheles aconitus is a secondary malaria vector and An. hyrcanus group is not considered as a vector in Thailand. The density of An. minimus s.l. was positively related to forest mean patch size, various water and paddy field landscape metrics and negatively related to landscape diversity. Anopheles hyrcanus group was also positively related to water metrics. Anopheline species diversity was negatively related to landscape diversity. Forest fragmentation resulting from human economic activities often increases landscape heterogeneity, which may result in a reduction in anopheline species diversity, as was the case in this study. There are indications that the effect of fruit orchards on anopheline diversity might be different in the dry season compared to the wet season. Fruit orchard landscape metrics affected species diversity negatively in the dry season and positively in the wet season. One reason for this could be that pesticides are typically applied in fruit orchards during the dry season. The conversion of forests to fruit orchards is a major land-use change in northern Thailand. These results show the complexity of vector status in northern Thailand and that vector and agriculture pest control are intricately interrelated. It is therefore important to include both the public health and agricultural sectors in controlling malaria vectors in the country. Our results also indicate that if landscape management should be used for malaria control in northern Thailand large-scale reduction and fragmentation of forest cover would be needed. Such drastic actions do not agree well with current global objectives concerning forest and biodiversity conservation This revised version was published online in July 2006 with corrections to the Cover Date.     

Ontogenic development of enzymatic activity and digestive system in Jullien's golden carp (Probarbus jullieni Sauvage, 1880)

Ontogenic development of the main enzymes and histological structure of digestive organs were studied in Jullien's golden carp (Probarbus jullieni) from hatching until 50 days after hatching (DAH). The larval fish were produced by artificial insemination and fed only Moina sp. till end of experiment. Body weight (mg) and total length (cm) of Jullien's golden carp increased exponentially and linearly. The results indicate the fish weight grew fast with increasing rate, while length increased at a constant rate over the studied period. Up‐regulation of acid protease was observed in newly hatched larvae and the specific activity gradually decreased with time. Trypsin specific activity was relatively stable within the first 35 DAH, while fluctuations in chymotrypsin were observed. Among these three proteolytic enzymes, acid proteases exihibited relatively high specific activity in newly hatched larvae, suggesting a role in yolk protein degradation. Alkaline proteases became more prominent with age and correlated with an abrupt decrease in acid proteases. Increased lipase‐specific activity appeared within 3 DAH and then gradually decreased with time, indicating the capacity to digest yolk lipid reserve. Amylase and cellulase‐specific activities changed in a similar manner, and the sensitivity to time was higher in amylase than in cellulase. The digestive organs and accessory organs developed around 3–5 DAH. However, intestinal histology was almost fully developed around 18 DAH. These findings should be useful for deciding the preferred timing for weaning, as well as on developing artificial diets referenced to the physiological changes of digestive enzymes.