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1.
2.
Suitability of mapped sequence tagged microsatellite site markers for establishing distinctness, uniformity and stability in aromatic rice 总被引:6,自引:0,他引:6
R.K. Singh R.K. Sharma A.K. Singh V.P. Singh N.K. Singh S.P. Tiwari T. Mohapatra 《Euphytica》2004,135(2):135-143
At present, testing for distinctness, uniformity and stability (DUS) of crop varieties relies on a set of morphological characters.
These characters suffer fromthe limitations of number, interaction with the environment in which the variety grows and subjectivity
in decision-making. The potential of DNA-based markers such as sequence tagged microsatellite site (STMS), for establishing
DUS merits investigation. In the present study, a set of 55 mapped STMS markers, selected from 12 linkage groups of rice genome,
was used to examine distinctness of 23 aromatic rice genotypes including the commercially important Basmati varieties. Forty-one
of these markers (74.5%) showed polymorphism between the varieties. The number of alleles per locus ranged from 2–4 with an
average of 2.3. The polymorphism information content (PIC) of the markers varied from 0.083 to 0.665 with an average of 0.338.
All the varieties could be differentiated from each other at a low probability (0.07×10-13) of identical match by chance. The marker-based clustering of the varieties corresponded with the known phenotypic classification,
thereby providing confidence in the distinctness established by the mapped STMS markers. The utility of these markers to study
uniformity and stability was analysed using a commercially important crossbred Basmati rice variety Pusa Basmati 1(IET-10364)
that contributes about 40–50% of Basmati rice export from India. Genotyping of twenty individual plants, grown from the nucleus,
breeder, foundation, certified and farmer's saved seed samples using all the 55 markers revealed no variation among the plants.
These observations suggested that the set of mapped markers employed in this study could be further used for establishing
distinctness of aromatic rice varieties and for studying DUS of the important commercial variety Pusa Basmati 1.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
3.
Munir K Muneer MA Tiwari A Chaudhry RM Muruganandan S 《Veterinary research communications》2007,31(7):909-929
Immunization against Angara disease virus (ADV), a serotype 4 avian adenovirus, and Newcastle disease virus (NDV), an avian
paramyxovirus serotype 1, is the mainstay of a broiler vaccination programme, while polyether ionophores usually form an essential
component of a broiler medication programme in most parts of India and Pakistan. The role of polyether ionophores in the protective
immune responses of broiler chickens vaccinated and challenged with ADV and NDV was investigated. A total of 1600 birds were
divided into eight groups of 200 birds each. First four groups were vaccinated against NDV and ADV, while the remaining four
served as unvaccinated controls. The first 3 groups of birds were administered salinomycin, monensin and cyclophosphamide
(CYP), respectively. The last group served as an untreated control. The same treatment schedule was also followed for the
next four unvaccinated groups. The post-vaccination and post-challenge serological responses to NDV and ADV, body and lymphoid
organ weight gains, post-challenge survival rate and detection of NDV and ADV in the tissues of infected birds were evaluated.
Birds administered salinomycin showed a significant stimulation of protective immune responses against both NDV and ADV as
compared to the untreated and CYP-treated birds. Monensin also enhanced the protective immune responses against both viruses
but the effect was not statistically significant. Thus, it is concluded that monensin and salinomycin augment the anti-NDV
and anti-ADV immune responses in broiler chickens, which supports their use in poultry flocks. 相似文献
4.
Mishra N Vilcek S Rajukumar K Dubey R Tiwari A Galav V Pradhan HK 《Research in veterinary science》2008,84(3):507-510
Since cattle are widely infected by bovine viral diarrhea virus (BVDV) in India, we searched for pestivirus infection in yaks. Of 71 pure and crossbred yaks from Himalayan region, pestivirus antigen was detected by Ag-ELISA in three animals. Pestivirus in leukocyte and cell culture isolated virus samples originating from positive yaks was also confirmed by RT-PCR using panpestivirus specific primers selected from 5'-untranslated region (5' UTR). The 5' UTR, N(pro) and E2 regions were sequenced and used for genetic typing. Phylogenetic analysis revealed that pestiviruses detected in three Himalayan yaks were similar genetically, belonging to BVDV-1. Antigenic characterisation of yak pestivirus also confirmed the typing as BVDV-1. This is the first report on the identification of BVDV type 1 in yaks. 相似文献
5.
6.
Evaluation of hyperimmune sera against goat pox viral antigens 总被引:1,自引:0,他引:1
Tropical Animal Health and Production - 相似文献
7.
Kataria RS Tiwari AK Nanthakumar T Goswami PP 《Veterinary research communications》2001,25(5):429-436
A single-tube, non-interrupted, one-step RT-PCR has been standardized to amplify the hypervariable region of the VP2 gene sequence of infectious bursal disease virus (IBDV). The technique standardized on purified viral RNA was successfully applied to the detection of the virus directly in clinical samples. The amplified products were confirmed to be IBDV specific by their size in ethidium bromide-stained agarose gel, nested PCR and restriction enzyme digestion. Digestion of the amplicons with StyI restriction enzyme also differentiated classical virus from six very virulent field isolates. The sensitivity of the one-step RT-PCR was found to be 0.2 pg of viral RNA. 相似文献
8.
9.
Purushottam Kaushik Dhirendra K. Singh S. Vinoth Kumar Ashok K. Tiwari Gunjan Shukla Shanker Dayal Pallav Chaudhuri 《Veterinary research communications》2010,34(2):119-132
Brucella abortus, a gram negative, facultative intracellular pathogen causes brucellosis in many animal species and humans. Although live,
attenuated vaccines are available against this infection, they suffer from certain limitations. Therefore, the development
of an effective subunit vaccine against brucellosis is an area of intense research. The outer membrane proteins (OMPs) of
Brucella species have been extensively studied for its immunogenicity and protective ability. We have investigated the potential of
CpG ODN to enhance the immunogenicity and protective efficacy of recombinant 28 kDa outer membrane protein (rOMP28) of Brucella melitensis. The study demonstrated vigorous immunoglobulin G (IgG) response of OMP28. The administration of rOMP28 with CpG caused increased
cell mediated immune response in terms of induced IgG2a, T-cell proliferation and up-regulation of type I cytokine expression.
In contrast, the free antigen suppressed the interferon gamma (type I cytokine) production on in-vitro stimulation of spleenocytes.
The result indicates the role of OMP28 in the down regulation of IFN-γ production. Moreover, the B. abortus S-19 vaccinated mice showed highest production of IL-4 and IFN-γ. The protective ability of the antigen was evaluated by
systemic bacterial clearance after challenging the mouse with B. abortus 544 pathogen. The level of protection was significant in rOMP28+CpG treated mice but was lower than the required level. The
results of the present study indicate that rOMP28 could be an immunogen capable of inducing both humoral and cellular immune
response. The humoral response was biased towards Th1 type when it was co-administered with CpG. 相似文献
10.
J.A. VanLeeuwen J.P. Haddad I.R. Dohoo G.P. Keefe A. Tiwari H.M. Scott 《Preventive veterinary medicine》2010,93(2-3):129-138
Our objective was to determine cow- and herd-level risk factors associated with seropositivity for Neospora caninum in a large number of randomly selected Canadian dairy herds, controlling for important confounding variables and co-infections with bovine leukemia virus (BLV), bovine viral diarrhea virus (BVDV) and Mycobacterium avium subspecies paratuberculosis (MAP). Serum samples were obtained from 30 randomly selected cows, where available, in 240 herds using monthly milk testing, within 6 of 10 provinces, and these samples were tested for antibodies against BLV, MAP and N. caninum using commercially available ELISA test kits. Five unvaccinated cattle >6 months old from each herd were tested for antibodies to BVDV using virus neutralization. Most herd-level predictors were obtained through personal interviews with questionnaires administrated to each farm manager. A mixed logistic-regression model was built using N. caninum serostatus at the cow-level as the outcome variable, with herd as a random effect and province as a fixed effect. A BLV seropositive cow was 1.50 times more likely to be seropositive for N. caninum than a BLV-seronegative cow, and this was the only cow-level variable to remain in the final model. Regarding herd-level variables, with “no on-farm dogs” as the baseline, “presence of dogs but not known to eat placentas and/or fetuses” increased the odds of seropositivity for N. caninum by a factor of 1.66. For “presence of dogs known to eat placentas and/or fetuses”, the odds ratio (OR) was 2.75, demonstrating a dose–response relationship. “Using embryo transfer” (OR = 0.69), “asking for a BVDV-negative test before introducing an animal” (OR = 0.30), “using monensin in dry cows” (OR = 0.71), and “heifers having nose-to-nose contact with calves” (OR = 0.73) were all dichotomous variables negatively associated with seropositivity for N. caninum. “Number of milk cows on the farm” (OR = 0.99), and “area (acres) used for forage production” (OR = 0.99) were continuous variables negatively associated with N. caninum seropositivity. 相似文献