Association between ABCB1-T1236C polymorphism and drug-resistant epilepsy in Iranian female patients

Background: One third of epileptic patients are resistant to several anti-epileptic drugs (AED). P-glycoprotein (P-gp) is an efflux transporter encoded by ATP-binding cassette subfamily B member 1 (ABCB1) gene that excludes drugs from the cells and plays a significant role in AEDs resistance. Over-expression of P-gp could be a result of polymorphisms in ABCB1 gene. We studied the association of T129C and T1236C single-nucleotide polymorphisms (SNP) of ABCB1 gene with drug-resistant epilepsy in Iranian epileptics. Methods: DNA samples were obtained from 200 healthy controls and 332 epileptic patients, of whom 200 were drug responsive and 132 drug resistant. The frequencies of the genotypes of the two SNP were determined by polymerase chain reaction followed by restriction fragment length polymorphism. Results: No significant association was found between T129C and T1236C genotypes and drug-resistant epilepsy neither in adults nor in children. However, the risk of drug resistance was higher in female patients with 1236CC (P = 0.02) or CT (P = 0.008) genotype than in those with TT genotype. The risk of drug resistance was also higher in patients with symptomatic epilepsies with 1236CC (P = 0.02) or CT (P = 0.004) genotype than in those with TT genotype. The risk of drug resistance was lower in patients with idiopathic epilepsies with 129TT genotype (P = 0.001) than in those with CT genotype. Conclusion: Our results indicate that T1236C polymorphism is associated with drug resistance in Iranian female epileptic patients. Replication studies with large sample sizes are needed to confirm our results. Key Words: ATP-binding cassette subfamily B member 1 (ABCB1), Drug-resistant epilepsy, Single nucleotide polymorphism     

Pistachio Production Management in Iran by Rest-breaking Treatment and Comparison of Dynamic vs Chill Hour Model for Chill Accumulation

Shortage of winter chill has reduced pistachio (Pistacia vera L.) fruit yield and quality from 2015 to 2018 in Iran. In this study accumulated chilling was determined by the chill hour (CH) and the dynamic (DM) model in five regions near Shahrebabak County, Kerman Province, Iran, for dormant season 2017–2018. The CH model has been routinely used in Iran for reporting chilling accumulation for pistachios. This study suggested that the DM better differentiated regional chill accumulations, the impact of chilling on phenological indices of growth and production between cultivars ‘Owhadi’ and ‘Akbari’, with and without rest-breaking (RB) treatments of Volk dormant oil. Volk oil treatment significantly improved vegetative and reproductive development, offset lack of chilling effects on pollination and nut filling, and may have advanced budbreak in some instances.