全文获取类型
收费全文 | 279篇 |
免费 | 37篇 |
国内免费 | 9篇 |
专业分类
林业 | 19篇 |
农学 | 17篇 |
基础科学 | 2篇 |
44篇 | |
综合类 | 55篇 |
农作物 | 37篇 |
水产渔业 | 78篇 |
畜牧兽医 | 46篇 |
园艺 | 11篇 |
植物保护 | 16篇 |
出版年
2024年 | 2篇 |
2023年 | 5篇 |
2022年 | 12篇 |
2021年 | 7篇 |
2020年 | 20篇 |
2019年 | 21篇 |
2018年 | 22篇 |
2017年 | 22篇 |
2016年 | 17篇 |
2015年 | 7篇 |
2014年 | 18篇 |
2013年 | 19篇 |
2012年 | 15篇 |
2011年 | 20篇 |
2010年 | 17篇 |
2009年 | 17篇 |
2008年 | 11篇 |
2007年 | 15篇 |
2006年 | 11篇 |
2005年 | 4篇 |
2004年 | 4篇 |
2003年 | 5篇 |
2002年 | 7篇 |
2001年 | 4篇 |
2000年 | 3篇 |
1999年 | 4篇 |
1998年 | 2篇 |
1992年 | 4篇 |
1990年 | 3篇 |
1988年 | 4篇 |
1968年 | 2篇 |
1963年 | 1篇 |
排序方式: 共有325条查询结果,搜索用时 15 毫秒
1.
Seasonal Changes in the Milt Quality of Waigieu Seaperch,Psammoperca waigiensis: Implications for Artificial Propagation
下载免费PDF全文
![点击此处可从《Journal of the World Aquaculture Society》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Milt quality of Waigieu seaperch was sampled at the beginning, the middle, and the end of the spawning season to assess if the sampling season has an effect on milt quality parameters. The milt volume and total sperm production were higher at the middle of the spawning season while the sperm concentration in milt was significantly higher at the beginning and the end of the spawning season. Sperm morphology and the major parameters of seminal plasma (pH, total protein, Mg2+, or Ca2+ concentrations) did not differ throughout the spawning season. The concentrations of Na+ and the osmolality increased throughout the spawning season. The percentage of motile cells did not differ during the spawning season while the duration of sperm motility (swimming duration) and velocity (swimming speed) were significantly different during the spawning season. The fertilization rate (75.7 ± 6.8%) and hatching rate (56.5 ± 3.1%) at the middle of the spawning season were higher than at the beginning and end periods. Results indicated that milt quality parameters and fertility success in the middle of the spawning season were higher compared to earlier and later sampling dates. Thus, we recommend collecting milt during this time for maximal fertilization and hatching success. 相似文献
2.
Thinh Tuan Chu John W.M. Bastiaansen Elise Norberg Peer Berg 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2018,68(1):1-10
To improve genetic gain of breeding programs for village poultry production, breeding schemes with observations obtained in village production systems using individual (VIO) and group recording (VGO) were examined under different levels of genotype-by-environment-interactions (GxE). GxE was modeled by varying the correlation between traits measured in the breeding station and village environments for bodyweight (rg_BW) and egg production (rg_EP). Relative and absolute genetic gains obtained from VIO and VGO were used for comparison between the schemes. Results showed that village observations significantly improved genetic gains compared to the scheme without birds tested in the village. The improvement was only slightly larger with individual observations than with group observations. Higher rg_BW and rg_EP led to lower relative genetic gain, but a higher absolute gain of VIO and VGO. It is recommended to apply a breeding scheme using group recording of village performance when strong GxE in breeding for village poultry is expected. 相似文献
3.
4.
Differences in uptake and killing of pathogenic and non‐pathogenic bacteria by haemocyte subpopulations of penaeid shrimp,Litopenaeus vannamei, (Boone)
下载免费PDF全文
![点击此处可从《Journal of fish diseases》网站下载免费的PDF全文](/ch/ext_images/free.gif)
V V Tuan J J Dantas‐Lima K V Thuong W Li K Grauwet P Bossier H J Nauwynck 《Journal of fish diseases》2016,39(2):163-174
Phagocytosis is an important function of both invertebrate and vertebrate blood cells. In this study, the phagocytic activity of haemocyte subpopulations of penaeid shrimp, Litopenaeus vannamei, (Boone), against pathogenic and non‐pathogenic particles was investigated in vitro. The haemocytes of penaeid shrimp were firstly separated by centrifugation on a continuous density gradient of iodixanol into four fractions with five subpopulations (sub), of which sub 1 (hyalinocytes) and sub 4 (semi‐granulocytes) have the main function in phagocytosis of both pathogenic and non‐pathogenic bacteria as well as fluorescent polystyrene beads. It was found that these haemocyte subpopulations engulfed virulent Vibrio campbellii and Vibrio harveyi at a higher rate than non‐virulent Escherichia coli and polystyrene beads. When these bacteria were mixed with shrimp haemocyte subpopulations and incubated for 180 min, the percentage of viable intracellular V. campbellii (25.5 ± 6.0%) recovered was significantly higher than the percentage recovered from V. harveyi (13.5 ± 1.1%). No viable intracellular E. coli was observed in this study. In contrast to V. harveyi and E. coli, V. campbellii containing endosomes did not acidify in time. Incubation of haemocyte subpopulations with the most virulent V. campbellii strain resulted in a significant drop in haemocyte viability (41.4 ± 6.3% in sub 1 and 30.2 ± 15.1% in sub 4) after 180 min post‐inoculation in comparison with the less virulent V. harveyi (84.1 ± 5.6% in sub 1 and 83.4 ± 4.1% in sub 4) and non‐virulent E. coli (92.7 ± 2.8% in sub 1 and 92.3 ± 5.6% in sub 4) and polystyrene beads (91.9 ± 1.6% in sub 1 and 84.4 ± 3.4% in sub 4). These findings may be a valuable tool for monitoring shrimp health and immunological studies. 相似文献
5.
Thi Thanh Nhan Pham Thi Ngoc Lan Nguyen Thi Ha Bui Huu Quan Nguyen Thi Tam Nguyen Van Son Le 《The Journal of Horticultural Science and Biotechnology》2019,94(5):591-598
Catharanthus roseus contains vincristine and vinblastine, which are outstanding drugs for cancer. In the biosynthetic pathways of terpenoid indole alkaloids (TIAs) in C. roseus, deacetylvindoline 4-O-acetyltransferase (DAT) is a key enzyme that catalyses the last reaction of vindoline biosynthesis to form vinblastine and vincristine. In this study, the CrDAT transgene was transferred into the periwinkle by Agrobacterium-mediated transformation and generated transgenic periwinkle lines with an increase in vincristine accumulation. The C. roseus DAT gene was introduced into C. roseus plants and it was confirmed that CrDAT was successfully transferred into the genome of periwinkle plants and efficiently translated to synthesise recombinant DAT protein. Four transgenic periwinkle lines in T1 generation, T1-1, T1-3, T1-6, and T1-7, expressed recombinant DAT protein with the total protein content in the range of 2.86 μg.mg?1 to 5.12 μg.mg?1. Moreover, the vincristine contents of four transgenic lines increased by 1.63?2.48-fold compared to non-transgenic plants, ranging from 6.91 µg.g?1 (fresh weight) to 10.53 µg.g?1 (fresh weight). The T1-1 line had the highest vincristine content. Hence, the overexpression of the recombinant DAT protein can improve the vincristine accumulation of transgenic C. roseus plants.Abbreviation: CrDAT - Catharanthus roseus Deacetylvindoline-4-O-Acetyl Transferase; D4H - Deacetoxyvindoline 4-hydroxylase; ELISA - Enzyme-Linked Immunosorbent Assay Monoterpene indole alkaloid; T0, T1 - Generations of transgenic plants; TIAs - Terpenoid indole alkaloids; WT- The wild-type tobacco plants (non transgenic plant); 35S - Cauliflower mosaic virus 35S promoter 相似文献
6.
Effects of Thyroxin and Domperidone on Oocyte Maturation and Spawning Performances in the Rabbit Fish,Siganus guttatus
下载免费PDF全文
![点击此处可从《Journal of the World Aquaculture Society》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Thyroxin (T4) is proven to have various functions in fish reproduction. In this study, we investigated oocyte maturation and spawning performance in the rabbit fish, Siganus guttatus, orally exposed to different doses of T4 (0.05, 0.25, and 0.5 mg/kg fish) during vitellogenesis. No statistically significant differences of reproductive characteristics were observed among the treatments. However, the group that was exposed to the dose of 0.5 mg/kg spawned after receiving a hormone injection. The findings suggest that feeding T4 during vitellogenesis did not advance oocyte maturation, but probably could induce spawning in rabbit fish. In a separate experiment, we investigated the effects of orally administered domperidone (DOM) on reproductive performances of the female rabbit fish. The study showed that oral administration of DOM during the early stages of the reproductive cycle had no significant effects on oocyte maturation and spawning performance. Overall, these findings suggest that DOM may not be needed for the induction of maturation and spawning of this species under aquaculture conditions. These practical investigations were conducted with a view to attempt reducing drug use, production costs, and support sustainable aquaculture. 相似文献
7.
E.G. Wulff A.T.H. Pham M. Chérif P. Rey Y. Tirilly J. Hockenhull 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(1):69-76
Hydroponically grown cucumber (Cucumis sativus) seedlings were inoculated with zoospores of 1 mycoparasitic (Pythium oligandrum) and 2 pathogenic (Pythium aphanidermatum and Pythium group F) Pythium spp. During the first 2 days after inoculation, all the Pythium spp. caused reduction in the root length. However, roots treated with Pythium oligandrum quickly reached the length of the control and on the 8th day, and for the rest of the experimental period, stimulation of root elongation was noted. Pythium oligandrum was not pathogenic on cucumber and no differences in the fresh weights of control and Pythium oligandrum inoculated plants were observed in the course of the experiment. Pythium group F and Pythium aphanidermatum were pathogenic on cucumber seedlings, but their pathogenicities differed. Thus, while Pythium group F had a constant, negative influence on root length and plant growth, measured as fresh weight, Pythium aphanidermatum caused generalized necroses of the root system, inhibiting consistently root elongation and plant growth and finally causing plant death. Moreover, the zoospores of 2 mycoparasitic species, Pythium oligandrum and Pythium periplocum, were not attracted to roots of cucumber and accumulated on the roots in very low numbers compared to those of the pathogenic species, Pythium aphanidermatum, which were strongly attracted and accumulated in large numbers. Finally, it was also found that Pythium oligandrum colonized the roots very poorly, while Pythium group F and Pythium aphanidermatum were significantly better root colonizers. The significance of these findings is discussed in relation to the ecology of Pythium species and biocontrol. 相似文献
8.
A new ent-kaurane diterpenoid (1) was isolated from the leaves of Croton tonkinensis. The structure of 1 was determined as ent-7beta-hydroxy-15-oxokaur-16-en-18-ol from spectroscopic methods. 相似文献
9.
Detection of Novel Reassortant Influenza A (H3N2) and H1N1 2009 Pandemic Viruses in Swine in Hanoi,Vietnam
下载免费PDF全文
![点击此处可从《Zoonoses and public health》网站下载免费的PDF全文](/ch/ext_images/free.gif)
T. D. Dao N. T. Pham B. J. Cowling M. Peyre M. Peiris 《Zoonoses and public health》2015,62(6):429-434
From May to September 2013, monthly samples were collected from swine in a Vietnamese slaughterhouse for influenza virus isolation and serological testing. A(H1N1)pdm09 viruses and a novel H3N2 originating from reassortment between A(H1N1)pdm09 and novel viruses of the North American triple reassortant lineage were isolated. Serological results showed low seroprevalence for the novel H3N2 virus and higher seroprevalence for A(H1N1)pdm09 viruses. In addition, serology suggested that other swine influenza viruses are also circulating in Vietnamese swine. 相似文献
10.
利用60 Coγ射线对紫色马铃薯品种黑美人萌芽薯块进行诱变处理。诱变材料表型鉴定结果表明,辐照剂量与出苗率呈显著负相关,其半致死剂量为70Gy,致死剂量为80Gy。辐照处理能显著延缓出苗时间,导致叶片减少、茎杆变细、植株变矮。高剂量处理可提高分枝数。与CK相比,各处理结薯数差异不显著。单株产量随辐照剂量增加而降低,CK处理显著高于其他处理。在表型鉴定的基础上,利用SSR分子标记对辐射材料进行DNA检测。10对引物集团选择结果表明,6对具有多态性,共获得10条多态性条带,平均每对引物有1.7条。单株检测共获得扩增条带28条,其中8条有多态性,多态性比率为28.9%。说明,辐射可引起紫色马铃薯黑美人表型和DNA水平的变异。 相似文献