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2.
The aim of present experiment was to evaluate the plasma concentrations of estrone sulfate (E(1)S) and progesterone (P(4)) during late gestation in recipient cows transferred embryos produced by nuclear transfer (NT) and in vitro fertilization (IVF). Blood samples were collected from recipients transferred embryos produced by NT (n=9) and IVF (n=13) at 160, 220, 240, 260 and 270 d of gestation and then at 5 d intervals until parturition. Plasma samples were analyzed for E(1)S and P(4) by ELISA. One NT and three IVF cows aborted between days 220 and 260 of gestation. Two NT and one IVF cow had prolonged gestation (over 290 d). One IVF cow had an overweight fetus (50 kg) after abortion (257 d). The patterns of changes in the concentrations of E(1)S during late gestation in the NT and IVF cows were almost identical. The NT and IVF cows that aborted had prolonged gestation and much higher E(1)S concentrations than the average. One NT cow aborted after 220 d of gestation and had a sudden high increase in its E(1)S concentration from 160 d to 220 d of gestation. The NT and IVF cows that had prolonged gestation also had significantly higher (P<0.05) P(4) concentrations than the average. These results raise the possibility that the E(1)S and P(4) profiles can be used to monitor some late gestational problems, such as higher birth weight, abortion and prolonged gestation. 相似文献
3.
Zinnat A. Begum Ismail M. M. Rahman Hikaru Sawai Satoshi Mizutani Teruya Maki Hiroshi Hasegawa 《Water, air, and soil pollution》2013,224(3):1-21
Development of aminopolycarboxylate chelants (APCs) having enhanced biodegradability is gaining increasing focus to replace the EDTA and its homologs with those used widely for the ex situ treatment of contaminated soils and are potential eco-threats. The paper reports the chelant-assisted extraction of the toxic metals (Cd, Cu, Pb, and Zn) from the metal-spiked European reference soils (Eurosoil 1 and Eurosoil 4) using biodegradable APCs, namely EDDS, GLDA, and HIDS. The effects of chelant-to-metal molar ratio, solution pH, and metal/chelant stability constants were evaluated, and compared with that of EDTA. The selectivity aptitude of the biodegradable chelants towards the toxic metals was assumed from the speciation calculations, and a proportionate correlation was observed at neutral pH. Pre- and post-extractive solid phase distributions of the target metals were defined using the sequential extraction procedure and dissolution of metals from the theoretically immobilized fraction was witnessed. The effect of competing species (Al, Ca, Fe, Mg, and Mn) concentrations was proven to be minimized with an excess of chelant in solution. The highlight of the outcomes is the superior decontamination ability of GLDA, a biodegradable APC, at minimum chelant concentration in solution and applicability at a wide range of pH environments. 相似文献
4.
Shigeya Maeda Hisao Kuroda Koshi Yoshida Kenji Tanaka 《Paddy and Water Environment》2017,15(2):263-276
A method for allocating allowable ranges of total nitrogen (TN) load to nonpoint (diffuse pollution) sources in a watershed has been developed by adopting the two-phase grey fuzzy optimization approach. Competing goals of water quality management authorities and TN load dischargers at nonpoint sources such as paddy field, upland crop field, and residential area are described with linear imprecise membership functions including interval numbers. TN load discharged from each cell of the nonpoint sources is assumed to be transported along with surface, subsurface, and river flow under the conventional first-order kinetic removal with respect to distance. The travel length of the load is estimated with a digital elevation model in a geographic information system (GIS). Uncertainty of river discharge and self-purification coefficients appearing in the TN transport model is also expressed with interval numbers. The GIS-aided grey fuzzy optimization model developed here is applied to the Seimei River watershed, Japan. By solving the optimization model, the allowable load represented by an interval number at each cell is procured, which would be a scientific base for effluent control regarding nonpoint sources in the area. 相似文献
5.
Yano S Umeda D Maeda N Fujimura Y Yamada K Tachibana H 《Journal of agricultural and food chemistry》2006,54(14):5203-5207
Flavonoids ubiquitously exist in plants, vegetables, fruits, and teas. We evaluated the effect of dietary apigenin, one of the well-known flavonoids, on the immune system in C57BL/6N mice. Mice were fed experimental diets containing apigenin for 2 weeks. After the experimental period, there was no significant difference in body and organ weights between the control and the apigenin group. The total immunoglobulin (Ig) E levels in mice fed apigenin were significantly suppressed, whereas levels of IgG, IgM, and IgA were not affected. We also examined the effect of the apigenin diet on cytokine expression in mice sera using a cytokine array. The production of regulated upon activation normal T cell expressed and secreted (RANTES) and soluble tumor necrosis factor receptor I (sTNFRI) in mice sera was down-regulated by the apigenin diet. These results suggest that a diet containing apigenin can reduce serum IgE and inflammatory cytokines such as RANTES and sTNFRI in mice. 相似文献
6.
通过温室盆栽试验 ,分析和探讨了三个水平的土壤水分条件对分蘖期和成熟期收获的旱稻(OryzasativaL .)生物量累积、水分利用率 (WUE)、植株不同部位的碳同位素识别值 (CID)的影响 ,并了解了它们之间的相互关系。水分条件包括 :饱和含水量 (W1 )、饱和含水量的 70 %(W2 )、饱和含水量的 4 0 %(W3)。结果表明当土壤水分条件从W1降低到W2时 ,分蘖期收获的生物量降低 4 5 %左右 ,成熟期收获的生物量降低 1 6 %~ 1 9%;而当从W1降低到W3时 ,分蘖期收获的生物量降低 73%左右 ,成熟期收获的生物量降低 5 5 %~ 5 7%。然而 ,根据地上部干重计算而来的WUE(WUE 地上部 )和根据全株干重计算而来的WUE(WUE 全株 )则随土壤含水量的降低而增加 ,其增幅在分蘖期为 0 .0 7~ 0 .2 8gkg-1 ,在成熟期为 0 .0 7~ 0 .4 5gkg-1 。植株的CID值变幅为 1 7.0~ 2 0 .6 ,但植株不同部位间差别显著 ,分蘖期收获的样品CID值从小到大的顺序为 :根 <最近完全伸展叶 <叶芽 <茎秆 ;而成熟期收获的样品CID值从小到大的顺序为 :籽粒 <根 <茎秆 <旗叶。随着土壤含水量的降低 ,植株所有部位的CID值亦显著减小。叶部的CID值与WUE 地上部(和WUE 全株 )之间呈一致的负相关关系。 相似文献
7.
Ichiro Nagaoka Hideki Sasahara Kei Matsushita Hideo Maeda Shuichi Fukuoka Utako Yamanouchi 《Plant Production Science》2013,16(4):546-553
ABSTRACTWe conducted a quantitative trait locus (QTL) analysis of grain appearance (GA) and agronomic traits of rice, using 128 recombinant inbred lines derived from ‘Emi-no-kizuna’ and ‘Tomohonami’. We detected two promising QTLs associated with GA: qGA4 on chromosome 4 and qGA8 on chromosome 8. qGA4 contributed highly to the greater percentage of perfect grains of the Emi-no-kizuna genotype. In the same region, we detected other QTLs associated with panicle number and spikelet number per panicle. In near-isogenic lines (NILs) in which Emi-no-kizuna alleles were introgressed in the genomic region of only the semi-dwarf 1 (sd1) locus (NIL_1) and both the sd1 locus and qGA8 (NIL_2), respectively, the percentage of perfect grains was significantly higher and the percentages of milky white, basal white, and white back grains were significantly lower than in Tomohonami; and the percentages of milky white and white back grains of NIL_2 were significantly lower than those of NIL_1. These results suggest that introgression in the sd1 region could improve GA, and that the addition of qGA8 could further improve GA. The culm lengths of the NILs were significantly shorter than that of Tomohonami, indicating improved lodging resistance. Grain weight of NIL_2 was significantly smaller than that of NIL_1, suggesting that the effect of qGA8 could be pleiotropic, or the gene that underlies qGA8 could be linked with genes associated with grain weight.Abbreviations: ANOVA: analysis of variance; AT20: mean air temperature in the 20 days after heading; BW: basal white grain; CL: culm length; DAH: days after heading; GA: grain appearance; GW: 1000-grain weight; LOD: logarithm of odds; MW: milky white grain; NIL: near-isogenic line; PG: perfect grain; PL: panicle length; PN: panicle number; PTSN: putative total spikelet number; PVE: percentage of phenotypic variation explained; QTL: quantitative trait locus; RIL: recombinant inbred line; SN: spikelet number per panicle; SNP: single nucleotide polymorphism; WB: white back grain 相似文献
8.
Watanabe H Koyama T Omata Y Uzuka Y Tanabe S Sarashina T Maeda R Saito A 《Veterinary parasitology》2001,99(4):287-295
In order to examine the antigenic similarity and specificity of the trail antigen of Eimeria stiedai and Etp 100, a microneme protein of Eimeria tenella, monoclonal antibodies to the trail antigen of E. stiedai sporozoites were selected by an indirect immunofluorescent antibody method. The monoclonal antibody of one clone, 3D10, reacted with the anterior portion of non-fixed sporozoites. By immunoblotting, the monoclonal antibody was found to react with a 100 kDa antigen of E. stiedai sporozoites, and a 117 kDa antigen of E. tenella sporozoites and merozoites. It was also found to react with a recombinant protein with thrombospondin-/properdin-like motifs homologous to E. tenella microneme protein Etp 100. The monoclonal antibody significantly inhibited the penetration of E. stiedai sporozoites into cultured rabbit hepatobiliary epithelial cells. These results suggest that E. stiedai sporozoites have a trail antigen, located in the anterior region on the outer surface of the sporozoites, which has an epitope with thrombospondin-/properdin-like motifs similar to E. tenella microneme protein Etp 100. This protein may play an important functional role in the process of penetration of host cells. 相似文献
9.
Yabuki A Maeda M Suzuki S Matsumoto M Kurohmaru M Hayashi Y Taniguchi K Nishinakagawa H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(4):461-465
Although mice kidney morphology shows various sexual dimorphisms, the effect of the estrous cycle has not previously been discussed. In this study, we investigated the effects of the estrous cycle on kidney morphology, including renin-positive areas, of female DBA/2 mice. No effects were confirmed in most of the histometrical parameters, however, the percentage of the renal corpuscles in which cuboidal epithelium covered under 50% of the parietal layer was significantly higher during estrus compared to that during anestrus. 相似文献
10.
Rupasinghe V Tajima T Maeda K Iwatsuki-Horimoto K Sugii S Horimoto T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1999,61(11):1253-1255
We used a consensus primer PCR method to amplify a region of herpesviral DNA-directed DNA polymerase gene using degenerate primers for initial characterization of the porcine cytomegalovirus (PCMV) genome. The sequence of the PCR product from PCMV DNA template and its alignment with other herpesvirus DNA polymerase counterparts showed that both conserved amino acid residues and conservative amino acid substitutions are in parallel. Phylogenetic analysis revealed that PCMV should be included in the clade comprising human herpesvirus 6 and 7, rather than human and mouse cytomegaloviruses, in Betaherpesvirus subfamily. 相似文献