紫外分光光度法控制酥黄注射液中蟾酥质量

根据吲哚衍生物与对二甲氨基苯甲醛溶液反应生成有色物质原理，采用紫外分光光度法，通过测定该有色物质的吸收度来控制蟾酥的质量。方法简便快速．效果较好。     

我国部分地区ＮＤＶ的分子流行病学研究

本研究根据新城疫病毒（ＮＤＶ）Ｆ基因编码区１－３７４位核苷酸序列计算其遗传距离并给出了ＮＤＶ的系统发育进化树，将６８株ＮＤＶ分为９个基因型（３０株为国内分离株），其中Ⅰ－Ⅵ是早已存在的老基因型，Ⅶ、Ⅷ、Ⅸ为新发现的基因型，特别是Ⅸ为我国特有的基因型（Ｆ４８ＥＯ、Ｍ３、ＨＬＪ－３、ＨeB-1P和ＮＭ－５）。１９９７－１９９９年我国云南、广西、甘肃、陕西、新疆等地分离的ＹＮ－１Ｐ、ＧＸ－３、Ｈ１、Ｈ２、Ｐ１、ＧＸ－１、ＧＸ－２、ＧＳ－３、ＳＨＸ－２、ＳＨＸ－３、ＳＨＸ－６、ＳＨＸ－７、ＸＪ－２和１９９１年分离的ＨuB-1均属于Ⅶ基因型，该基因型的病毒是９０年以来引起新城疫发生的主要病原。根据遗传距离和分离年代可将此基因型进一步划分为５个基因亚型，分别是Ⅶa、Ⅶb、Ⅶc、Ⅶd和Ⅶe。此外ＨuN-1/98、HLJ-4/95和ＨeH-1P属一个老的基因Ⅵ，１９７９－１９８５年分离自青海的ＱＨ－１、ＱＨ－２、ＱＨ－４属于一个新的基因型－Ⅷ型。可见在我国新城疫的流行是极其复杂的，既有老基因型的危害（Ⅰ－Ⅵ），又有新基因型（Ⅶ）的流行，更有我国独特Ⅷ和Ⅸ基因潜伏。     

Selection and amplification of the liver stem cell subset from rat bone marrow cells with a medium containing cholestatic serum in vitro

AIM: To explore the feasibility of direct separat and selective enlargement of the bone marrow-derived liver stem cells (BDLSC) from bone marrow cells with a culture system containing cholestatic serum in vitro. METHODS: Bone marrow cells of rats were cultured with selective media containing 2%, 5%, 7% and 10% cholestatic rat serum, respectively. The BDLSC were then induced to proliferate with the addition of hepatocyte growth factor (HGF) on the firth day. BDLSC were characterized using immunocytochemistry and RT-PCR for lineage markers, glycogen staining and urea synthetic assay for functions 2 weeks later. RESULTS: Bone marrow cells were unble to form colony in the presence of 2% cholestatic serum and apopotosis appeared gradually in 7% or 10% cholestatic serum. The BDLSC survived in the medium containing 5% cholestatic serum while the other types of cells did not. The survival cells proliferated with a high speed during the second week and then formed hepatocyte-like colony-forming units (H-CFU). Cells in the H-CFU expressed the characteristic proteins of fetal hepatocytes. Furthermore, they had glycogen storage and urea synthesis functions, two of the critical features of hepatocytes. CONCLUSION: The selective micro-environment effectively selected BDLSC from the bone marrow cell, and will be a new way to provide an abundant source of donor hepatocytes for clinical cell therapy.     

Effect of mutant β-catenin gene on the proliferation of hepatocytes

AIM:The β-catenin is a key molecule in the Wnt signal pathway, which plays a critical role in normal development and tumorigenesis. However, the mechanisms of the β-catenin on the cell growth control are still not completely defined. The aim of this study was to test the hypothesis that the mutant β-catenin may regulate the hepatocyte proliferation. METHODS: The immortalized murine hepatocyte cell line, AML12, was used for this study. A plasmid that contain mutant β-catenin S33Y was transfected into the AML12 cells and a stable cell line AML12S33Y was established. The cell growth property of this cell line and the parental cell were compared by flow cytometry analysis and direct cell count. The cells were also tested for the ability to form soft agar colonies, and the ability to form tumors in the severe immune deficient mice (SCID). RESULTS:1. The mutant β-catenin containing cell line AML12S33Y has higher proliferating index compared with the parental AML12 cells (P<0.01), suggesting that mutant β-catenin promotes cell growth. 2. The mutant β-catenin cells formed small colonies in soft agar after 4 weeks of culture, but did not generate tumor in SCID mice. CONCLUSION:The mutant β-catenin promotes liver cell growth.     

Effects of liver regeneration on the proliferation of rat fetal hepatocytes after intrasplenical transplantation

AIM:To study the effect of environment of liver regeneration on the proliferation of rat fetal hepatocytes after intrasplenical transplantation. METHODS:Fetal hepatocytes isolated from 3-week SD rat fetuses bred were transplanted into the spleens of liver regeneration model rats with 70% partial hepatectomy. The cell cycle of the hepatocytes in the remnants liver was analyzed by flow cytometer and the density dimensions of the donor fetal hepatocytes in spleen were measured by image analysis system 7 and 30 days post-transplantation, respectively. RESULTS:Compared with the control group, the proportions of S and G₂ /M cells in the remnants liver were obviously decreased (P＜0.05), but the density dimensions of the donor fetal hepatocytes in spleen increased significantly (P＜0.05) in rats with hepatectomy 7 days post-transplantation. CONCLUSION:The environment of liver regeneration is propitious to the proliferation of fetal hepatocytes after transplantation into spleen.     

旱作条件下转甜菜碱醛脱氢酶基因水稻籽粒灌浆特性的研究

在旱作条件下,对转甜菜碱醛脱氢酶(BADH)基因水稻的3个品系(52-7、51-15、51-22)、受体亲本中花8号(ck1)和当地推广的以色列陆稻白珍珠(ck2)的籽粒灌浆特性进行了比较分析。结果表明:5个品种(系)的强、弱势粒灌浆过程均符合Richards模型,强、弱势粒灌浆为异步灌浆型。三个转BADH基因品系与其受体亲本相比,强、弱势粒的灌浆速率提高,活跃灌浆期延长,灌浆高峰期的籽粒重增加;籽粒灌浆中、后期,强势粒的灌浆天数缩短,强、弱势粒的平均灌浆速率提高,且不同粒位籽粒灌浆速率差异减小,最终使不同粒位籽粒大小的差异缩小和粒重增加。最大灌浆速率、平均灌浆速率和灌浆高峰期的粒重与千粒重存在显著正相关。     

Bone marrow mesenchymal stem cells are induced to differentiate into cardiomyocytes by hepatocyte growth factor in vitro

AIM: To explore a new method of hepatocyte growth factor (HGF) inducing bone marrow mesenchymal stem cells (MSC) to differentiate into cardiomyocytes. METHODS: Bone marrow MSC was cultured with DMEM media (10% fetal calf serum) 4-6 passages, and induced by HGF (10 μg/L) for 30 d. Automatical beating of the differentiated cells was observed daily with transverse microscopy, or under condition of 0.1% isoproterenol or cal-cium-deprived incubation. Specific cardiac myosin in the cells was indentified by immunochemistry. RESULTS: At 14-20 d of differentiation, bone marrow mesenchymal stem cells formed clones, in 10%-50% of which spontaneous beating cell-mass had come to continuously exist. Isoproterenol increased the beating rate and calcium-deprived media inhibited the beating. The cells were identified to be cardiomyocytes by expression of cardiac myosin heavy chain. CONCLUSION: HGF may induce bone marrow mesenchymal stem cells into cardiomyocytes with high efficiency, but the differentiating pathway of stem cells remains to be further studied.     

大豆和百脉根古老原核基因的全基因组鉴定与比较分析

在漫长的进化过程中,高等真核生物从原核生物中获取了大量基因,对这些基因进行鉴定分析,可为研究高等植物系统演化及基因组水平比较分析提供理论依据。为了进一步解析大豆和百脉根古老原核基因在其基因组中所起的作用及进化关系,本研究利用生物信息学技术对大豆和百脉根基因组古老原核基因进行全基因组鉴定,并对其特征及功能进行了比较分析。研究结果表明,大豆(40.6%)古老原核基因的占比高于百脉根(33.9%),且多分布于内共生细胞器线粒体、叶绿体中。此外,古老原核蛋白质的结构域在大豆和百脉根基因组中的分布相似,表明其在大豆和百脉根进化过程中具有较高的共线性和保守性。通过GO注释发现大豆与百脉根中古老原核蛋白主要分布在膜系统、细胞、细胞组分中。在分子功能上,大豆古老原核蛋白更多地参与代谢和发育过程,这可能与其接受了更多的人为选育有关。在生物过程中,古老原核蛋白更多参与催化反应和结合反应,主要以酶的形式在发挥作用。通过比较分析,本研究揭示了古老原核蛋白在大豆和百脉根中进化的独特模式,可为其他豆科植物基因组分析提供一定理论基础。     

成品鞋柜的发展历程与存在问题

鞋柜是日常生活中使用频率非常高的一类家具。作为重要的收纳空间,鞋柜直接影响我们生活的便利和舒适性。笔者通过对成品鞋柜现状的考察,分析了目前成品鞋柜存在的不足,旨在为相关鞋柜生产企业的研发、销售提供借鉴与参考,以使成品鞋柜产品更好地适应现代城市家居的需要。     

上海城市绿地中景观灯配置对植物的影响

通过对上海主要绿地中景观灯与植物配置的调查分析,结果表明,悬挂造型灯的饰景照明、缠绕或覆盖灯串的轮廓照明以及运用泛光灯的泛光照明是景观灯与植物配置结合应用最广泛的形式;金属卤化物灯、高压钠灯和LED是目前上海植物造景中应用较多的景观灯类型;灯光颜色以黄、绿、红、蓝紫色系为主;景观灯的光照强度因光源类型及与植物的距离而存在差异,文章还分析了常见的景观灯配置形式对植物可能产生的影响。