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1.
以国内部分圈养的健康华南虎Panthera tigris amoyensis作为研究对象,通过收集华南虎血液生化报告和采集华南虎血液样本检测建立样本数据,血样检测采用自动血液生化分析仪干式试剂片生化技术测定,测定血液生化指标包括血糖(GLU)、总蛋白(TP)、总胆固醇(CHOL)、总胆红素(TBIL)、肌酐(CREA)等。得到华南虎血液生化共38项指标的正常参考值范围(95%概率),其中几项主要指标正常值范围如下:GLU(2.82-11.55)mmol/L、TP(56.58-85.93)g/L、CHOL(3.32-8.66)μmol/L、TBIL(0-5.30)μmol/L、CREA(52-349)μmol/L等。统计结果显示,雌雄华南虎间血液生化指标的部分项目有差异(P <0.05),幼龄组和成年组间各项生化指标差异不显著(P >0.05),与其他虎亚种相关血液生化报道的生化指标部分有差别。华南虎血液生化指标正常参考值范围不同于其他虎亚种,建立华南虎血液生化指标正常值对华南虎的饲养管理、疾病诊治预防和保育繁殖研究工作有重要作用。 相似文献
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利用黄淮地区的12个高产夏大豆品种(系),通过NCⅡ交配设计,对12个主要农艺性状的遗传进行了分析。结果表明,所有性状的加性方差大于显性方差。主茎节数、株高、每簇荚数和主茎荚数的加性成分占绝对优势,遗传力较高。分枝荚数、单株粒数和单株粒重的显性成分比例大,遗传力较低,其中单株粒重趋于完全显性。主茎节数的显性成分很少,其它性状均为部分显性。建立了单株产量与产量因子之间的逐步回归方程。 相似文献
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一次防治大豆灰斑病籽粒灰斑 总被引:1,自引:1,他引:0
通过室内及田间大豆不同生育期接种试验证明,籽粒感染灰斑病的关键时期是R3-R5期.R2期以前侵染不造成籽粒斑驳,据此提出一次防治大豆籽粒灰斑病的关键时期为R2-R4期. 相似文献
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Comparison of morphology, viability, and function between blood and milk neutrophils from peak lactating goats
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![点击此处可从《Canadian journal of veterinary research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Sui Zhi Tian Chai Ju Chang Chih Chi Chiang Huo Cheng Peh Mu Chiou Huang Jai-Wei Lee Xin Zhao 《Canadian journal of veterinary research》2005,69(1):39-45
The morphological features of blood and milk neutrophils from peak lactating goats were compared using light microscopy, scanning electron microscopy and flow cytometry in order to investigate the cytological changes of neutrophils after migration into the mammary gland. The kinetics of reactive oxygen intermediates (ROI) generation and gelatinase release of blood and milk neutrophils, with or without stimulation of phorbol 12-myristate, 13-acetate ester (PMA), were used to characterize their responses to inflammatory stimuli. Neutrophils isolated from goat milk were highly segmented and contained multi-lobed nuclei. Ultrastructurally, milk neutrophils were more ruffled on the surface compared to blood neutrophils. Approximately 30% of milk neutrophils were undergoing cell death, either necrosis or apoptosis, in contrast to 8% of blood neutrophils. The ROI production of activated milk neutrophils peaked earlier than blood neutrophils, but the duration and the intensity were much less. Neutrophils from both sources augmented the release of gelatinase in response to PMA (1 ng/mL). However, the amount of gelatinase released from milk neutrophils was lower (P < 0.05) than that of blood neutrophils. In summary, more neutrophils become apoptotic and necrotic in the mammary gland, presumably due to spontaneous aging, the process of diapedesis, and the interaction with milk components. Milk neutrophils have impaired functionalities in comparison with blood neutrophils. The information is relevant when studying mammary gland immunity and related diseases, such as mastitis. 相似文献
8.
棉铃虫对拟除虫菊酯类杀虫剂抗性遗传力的分析 总被引:3,自引:5,他引:3
采用域性状分析法,估算了棉铃虫对氰戊菊酯、三氟氯氰菊酯和溴氟菊酯的抗性现实遗传力,并对3种菊酯的抗性风险进行了评估。结果表明,棉铃虫对氰戊菊酯、三氟氯氰菊酯和溴氟菊酯的抗性现实遗传力分别为0.2027、0.1554和0.1084。假设田间种群的抗性现实遗传力为估算值的一半,杀死率为80%,预计抗性增长到10倍时,氰戊菊酯可使用14次,三氟氯氰菊酯可使用19次,溴氟菊酯可使用31次。三氟氯氰菊酯和溴氟菊酯的抗性风险明显低于氰戊菊酯;棉铃虫对含氟菊酯的抗性发展速度明显低于不含氟的菊酯。试验结果为实际应用提供了理论依据。 相似文献
9.
农杆菌介导的蝴蝶兰基因转化系统的建立 总被引:9,自引:1,他引:9
针刺后的蝴蝶兰‘White Hikaru’的类原球茎(PLB),与含绿色荧光蛋白基因( )和潮霉素磷酸转移酶基因(^p£)的pCAMBIA 1300一SmGFP的根瘤农杆菌LBA4404 共培养,培育出了转基因的蝴蝶兰。经绿色荧光蛋白检测和Southern印迹,证实了再生植株中含cop基因和hpt基因。 相似文献
10.
Comparative expression profile of microRNAs and piRNAs in three ruminant species testes using next‐generation sequencing
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![点击此处可从《Reproduction in domestic animals》网站下载免费的PDF全文](/ch/ext_images/free.gif)
microRNA (miRNA) and piwi‐interacting RNA (piRNA) are two classes small non‐coding regulatory RNAs that play crucial roles in multiple biological processes such as spermatogenesis. However, there are no published studies on conjoint analysis of miRNA and piRNA profiles among cattle, yak and their interspecies (the dzo) using sequencing technology. Next‐generation sequencing technology was used to profile miRNAs and piRNAs among those three ruminants to elucidate their functions. A total of 119, 14 and six differentially expressed miRNAs were obtained in cattle vs. dzo, cattle vs. yak and yak vs. dzo comparison groups, while there were 873, 1,065 and 1,158 differentially expressed piRNAs in those three comparison groups. The expression of three miRNAs was validated in the three ruminants, and the results suggested that the miRNA expression profiles data could represent actual miRNA expression levels. Moreover, the putative targets of differentially expressed miRNAs were predicted by their own genome, it is worth to note that both the cattle and yak genome were used for dzo, then the targets were subjected to GO enrichment and KEGG pathway analysis, revealing the likely roles for these differentially expressed miRNAs in spermatogenesis. In conclusion, this study provided a useful resource for further elucidation of the miRNAs and piRNAs regulatory roles in spermatogenesis. It may also facilitate the development of therapeutic strategies for dzo reproduction research. 相似文献