The risk of salmonellae shedding by dogs fed Salmonella-contaminated commercial raw food diets

Twenty-eight research dogs were enrolled to determine the prevalence of salmonellae shedding after consumption of 1 Salmonella-contaminated commercial raw food diet meal. Sixteen dogs were exposed to Salmonella-contaminated commercial raw food diets and 12 to Salmonella-free commercial raw food diets. Seven of the exposed dogs shed salmonellae 1-7 days after consumption of Salmonella-contaminated raw food diets. None of the dogs fed Salmonella-free diets shed salmonellae. No clinical signs were observed in either group. Five of the 7 dogs shed the same serotypes as those recovered from food samples used for feeding. Results showed the same serotypes and antimicrobial resistance pattern in 2 of the 7 shedders. Dogs fed Salmonella-contaminated raw food diets can shed salmonellae and may, therefore, be a source of environmental contamination potentially leading to human or animal illness.     

Evaluation of iohexol clearance used to estimate glomerular filtration rate in clinically normal foals

OBJECTIVE: To determine whether pharmacokinetic analysis of data derived from a single i.v. dose of iohexol could be used to predict creatinine clearance and evaluate simplified methods for predicting serum clearance of iohexol with data derived from 2 or 3 blood samples in clinically normal foals. ANIMALS: 10 healthy foals. PROCEDURE: Serum disposition of iohexol and exogenous creatinine clearance was determined simultaneously in each foal (5 males and 5 females). A 3-compartment model of iohexol serum disposition was selected via standard methods. Iohexol clearance calculated from the model was compared with creatinine clearance. Separate limited-sample models were created with various combinations of sample times from the terminal slope of the plasma versus time profile for iohexol. Correction factors were determined for the limited-sample models, and iohexol clearance calculated via each method was compared with exogenous creatinine clearance by use of method comparison techniques. RESULTS: Mean exogenous creatinine clearance was 2.17 mL/min/kg. The disposition of iohexol was best described by a 3-compartment open model. Mean clearance value for iohexol was 2.15 mL/min/kg and was not significantly different from mean creatinine clearance. A method for predicting serum iohexol clearance based on a 2-sample protocol (3- and 4-hour samples) was developed. CONCLUSIONS AND CLINICAL RELEVANCE: Iohexol clearance can be used to predict exogenous creatinine clearance and can be determined from 2 blood samples taken after i.v. injection of iohexol. Appropriate correction factors for adult horses and horses with abnormal glomerular filtration rate need to be determined.     

The cytologic and histologic diagnosis of ureteral fibroepithelial polyp in a dog

A 6-year-old, intact male, brindled, 30-Lb English Bulldog presented to the Purdue University Veterinary Teaching Hospital with a recurrent history of hematuria, periuria, and lethargy that responded temporarily to antibiotic therapy. The work-up included a complete blood count, serum biochemical profile, complete urinalysis, diagnostic imaging (abdominal radiographs and ultrasound with contrast urography), and exploratory laparotomy. The diagnostic imaging findings and subsequent exploratory revealed a unilateral, intraluminal, right-sided, 3-cm ureteral mass extending from the proximal ureter into the renal pelvis. Subsequently, a unilateral right-sided ureteronephrectomy followed by biopsy with cytopathology/cytology (impression smears) and histopathology of the ureteral mass was performed. The cytopathologic interpretation was benign mesenchymal proliferation with mildly atypical urothelial cells. The association of this mass with vascular tissue and a benign nuclear appearance on cytology is similar to reports of fibroepithelial polyps (FEPs) and myxomatous tumors. Histopathology diagnosed the mass as an FEP. Cytopathology proved useful in the presumptive diagnosis of this benign urothelial polyp. To the authors' knowledge, this is the first report using cytopathology to depict and characterize FEPs in veterinary and human medical literature.     

First evidence of spawning migration by goldfish (Carassius auratus); implications for control of a globally invasive species

Goldfish (Carassius auratus) was one of the first fishes to be domesticated and has been widely introduced across the globe, but is now considered one of the world's worst invasive aquatic species. Surprisingly, there is a dearth of information on its spatial and temporal movement patterns, which hampers the development of effective control programmes. We examined the movement patterns of an introduced population of C. auratus in a south‐western Australian river using passive acoustic telemetry. The study population had a high residency index within the array (i.e. proportion of all days at liberty that, on average, each fish was detected by a receiver) with fish being detected on 64% of days. The individuals were also reasonably mobile, travelling a mean of 0.30 km (linear river kilometres).day^‐1 within the array, and one fish moved 231.3 km over the 365‐day study period (including 5.4 km in a 24 hr period). Importantly, C. auratus displayed significant seasonal movement patterns including a clear shift in habitats during its breeding period with most mature individuals being detected in an off‐channel wetland during that time. The results of this study strongly suggest that C. auratus undertook a spawning migration into a lentic habitat. These results have important implications for developing control programmes for the species, such as targeting connections to off‐channel lentic systems during its breeding period.     

Fetal and adult hematopoietic stem cells give rise to distinct T cell lineages in humans

Although the mammalian immune system is generally thought to develop in a linear fashion, findings in avian and murine species argue instead for the developmentally ordered appearance (or "layering") of distinct hematopoietic stem cells (HSCs) that give rise to distinct lymphocyte lineages at different stages of development. Here we provide evidence of an analogous layered immune system in humans. Our results suggest that fetal and adult T cells are distinct populations that arise from different populations of HSCs that are present at different stages of development. We also provide evidence that the fetal T cell lineage is biased toward immune tolerance. These observations offer a mechanistic explanation for the tolerogenic properties of the developing fetus and for variable degrees of immune responsiveness at birth.     

Determining corn nitrogen rates using multiple prediction models

Weather uncertainty and soil spatial variability impact nitrogen (N) cycling and corn (Zea mays L.) growth, making accurate N predictions a challenge. Field studies were conducted in Lansing, Michigan, to evaluate a computer model (i.e., Adapt-N), a preseason year-based model (i.e., maximum return to N [MRTN]), and a crop sensor model (i.e., active canopy sensor with algorithm) for recommending corn N rates. To determine site-specific economic optimum N rates (EONR), five N rates were also applied (0, 33%, 66%, 133%, and 166% of the suggested MRTN) as starter + sidedress (SD) at V4. In a wet year (i.e., 2015), Adapt-N increased V8 SD N rates 35 kg N ha^?1 relative to the MRTN V4 SD N application. Although the greater rate of N may have provided additional yield protection, no statistical yield differences were observed between the two models. The MRTN model increased partial factor productivity (PFP) 20% relative to Adapt-N. Limited expression of V8 corn N deficiency reduced crop sensor total N rates (21–56 kg N ha^?1) and yield (0.82–1.05 Mg ha^?1) relative to other models. In a drier year (i.e., 2016), N demand was reduced (EONR 64 kg N ha^?1 less than 2015), resulting in similar corn response to all three models. Despite differences in actual corn N rate recommendations, all three models resulted in similar economic net returns across study years.

Abbreviations: EONR, economic optimum nitrogen rate; MRTN, Maximum Return to Nitrogen; NUE, nitrogen-use efficiency; PFP, partial factor productivity; SBNRC, sensor-based nitrogen rate calculator; SD, side-dress     

Artificially regenerating longleaf pine in canopy gaps: initial survival and growth during a year of drought

Nitrogen fertilization in the nursery, along with altering the configuration of forest gaps, may improve the reforestation success of longleaf pine seedlings. During the very droughty 1998 growing season in Florida and Georgia, survival was higher under the forest canopy than in small (0.10 ha, 36 m diameter) and large (1.6 ha, 144 m diameter) canopy gaps. In the large gaps, survival of containerized seedlings was higher along the edges, particularly the SW edge. Shade from adult trees and the nurse effect of shrubs increased survival, while grass competition reduced survival. During dry years part of the “exclusionary zone” along the edge of canopy gaps (SW sector) may serve as a “survival zone”, at least in the short term. A model using oval-shaped gaps oriented from NW to SE, with an area of 0.25 ha is proposed to maximize the survival and growth of artificially regenerated longleaf pine seedlings.     

Effects of zinc fertilizer amendments on yield and grain zinc concentration under controlled environment conditions

The application of zinc (Zn) fertilizer to lentil is an agronomic strategy that has the potential to improve yield and enhance grain Zn concentration. A pot study was conducted to determine if Zn fertilizer applied to three popular Saskatchewan lentil cultivars could increase yield and concentration of Zn in the grain. The effects of soil and foliar applied Zn forms, including ZnSO₄, Zn chelated with EDTA, Zn lignosulphonate, and a control were evaluated. Forms of Zn were not found to significantly increase yield (P = 0.828) or grain Zn concentration (P = 0.708) in any of the lentil cultivars tested. Fertilization with soil applied ZnSO₄ resulted in significantly (P < 0.0001) higher amounts of residual available Zn in the soil relative to other Zn treatments. Soil fertilized with ZnSO₄ had 1.13 mg kg^?1 diethylenetriaminepentaacetic acid (DTPA)-extractable Zn compared to 0.84 mg Zn kg^?1 and 0.77 mg Zn kg^?1 in the soil and foliar applied chelated Zn, respectively.