Botrytis cinerea Infection of Grape Flowers: Light and Electron Microscopical Studies of Infection Sites

ABSTRACT Specific floral organs including the calyptra, stigma, and receptacle area of glasshouse-grown grapevines (Vitis vinifera cv. Cabernet Sauvignon) were inoculated with aqueous suspensions of Botrytis cinerea conidia, and the initial steps involved in colonization and infection of the host tissues were studied for several days postinoculation using light microscopy as well as scanning and transmission electron microscopy. Conidia germinated on all floral organs examined and became attached to the host surface within 48 h after inoculation. However, in all cases the vast majority of conidia accumulated in a channel-like gap between the ovary and the calyx that extended in a narrowing fashion into the flower interior where the ovary joined the receptacle. Very few conidial germ tubes were detected in the style following inoculation of the stigma, and no evidence for their growth toward the ovaries could be found. In contrast, hyphae were more abundant in the receptacle area, regardless of the site of inoculation. Tips of the calyx became necrotic and mycelium formed in the gap between ovary and calyx within 72 h following inoculation, providing a major point of colonization and infection. B. cinerea colonized dehiscent calyptras within 72 h of inoculation, providing a potential source of inoculum from calyptras that remained stuck in the cluster. The results suggest that the grape flower's receptacle area is the predominant site of infection for B. cinerea, although a minor portion of infections may also occur through the stigma and style.     

Toxic impact of the de-icing material to street greenery in Riga, Latvia

There is little information on the ecological composition of snow on roads as well as the toxic effect of de-icing salt on the ecological status of the street trees (Tilia x vulgaris H.) in Latvia. Tilia x vulgaris is one of the most widespread tree species of street greenery in the boreo-nemoral zone – in Central, Northern and Eastern Europe. This investigation was done to find out the accumulation of de-icing salt (main elements – sodium and chlorine) in the snow–soil–plant system of street greenery in the central part of Riga (2004–2005), in order to reveal the toxic effect on the ecological status of the street trees (Tilia x vulgaris). Snow, soil and leaf samples were collected from 27 study sites (eight objects) in the central part of Riga with intense traffic and from three control sites (one object) located in a park area. In a majority of cases, the status of street trees could be characterized as damaged. There was a high heterogeneity in the Na and Cl concentrations even along one street. Significantly, increased levels of Na and Cl in urban snow, soil and leaf samples were found in comparison with the background values. There was close correlation between the Na, Cl concentration in the leaves and the extent of leaf necrosis. Principal new information on the critical level for observation visual Tilia x vulgaris leaf necrosis was stated based on our results: 0.14% Na and 0.66% Cl concentrations in the leaves.     

A study of chemical characteristics of soil in relation to street trees status in Riga (Latvia)

The chemical composition of soil and appropriate supply of nutrients are very important factors for normal plant growth and development. Lime tree (Tilia x vulgaris H.), is a popular tree species used for urban landscaping in Europe. However, there is little information on the chemical element concentrations available to and recommended for T. x vulgaris in urban soils. The objectives of this study were: (1) to investigate the amount of nutrients, de-icing salts and heavy metals available for uptake by trees in the city centre on a seasonal scale; and (2) to assess the relationship between the vitality of T. x vulgaris and soil chemistry. The research was carried out in five streets and a park in Riga (Latvia) during 2005–2007. Plant-available concentrations of 17 nutrients and heavy metals were determined via 1 M HCl extraction. Soil Cl content, pH, and electrical conductivity were also measured.The investigation revealed a high heterogeneity in soil chemical composition. In total, the element concentrations in urban soils did not exceed values commonly found in urban environments. Higher concentrations of Na, Cl, Ca, Mg, Zn, Cu, and increased pH, but lower P and B concentrations were found in the street soils in comparison to the park soil. Significantly higher concentrations of Na, Cl, and Mg, and lower concentrations of K, Fe, Cu, and B, as well as unfavourable ratios of several element concentrations were found in the soils where more damaged street trees were growing. In addition, the recommended fertilization regimes are discussed.