轻型木结构建筑覆板的集中静载性能

介绍轻型木结构建筑覆板集中静载结构性能试验的装置、条件和方法,并进行国产人工林落叶松胶合板作轻型木结构覆板的集中静载性能研究.结果表明:与国外胶合板覆板具有相同厚度的国产落叶松屋面板和楼面板,其集中载荷性能均优于相关标准的要求.     

Expression of cyclooxygenase-2 in IL-1β-stimulated mesangial cells is medicated by NF-κB/IκB signal pathway

AIM: To investigate the role of NF-κB/IκB signal pathway in the regulation of cyclooxygenase-2 (COX-2) expression in human mesangial cells (HMC). METHODS: The PGE₂ concentration in supernatants of HMC was measured by radioimmunoassay. COX-2 mRNA and protein expression were determined by RT-PCR and Western blot. Electrophoretic mobility shift assay (EMSA) and Western blot were used to detect the activity of NF-κB and degradation of IκB. RESULTS: IL-1β significantly upregulated COX-2 expression and PGE₂ production in HMC. Significant up-regulation of NF-κB activation, nuclear translocation of p65 subunit, and degradation of IκB α and IκB β were observed in IL-1β-induced HMC. CONCLUSION: Expression of COX-2 in IL-1β-induced HMC is mediated by NF-κB/IκB signal pathway.     

Inhibition of K562 cell growth by antisense drug targeting with VEGF mRNA in vitro

AIM: To investigate inhibition of K562 cell growth by antisense drug targeted VEGF mRNA. METHODS: X7, 20-mer antisense sequences were selected, synthesized and modified with phosphorothioate. The drug was transfected into K562 cells in the present of lipofection. Cell growth was assayed by trypan blue dye exclusion assay and MTT. The level of VEGF protein in the media was determined by ELISA. The morphology of apoptotic cells were observed by Giemsa staining, and the propotion of apoptotic cells was detected by flow cytometry. RESULTS: The antisense drug inhibited growth of K562 and downregulated expression of VEGF protein significantly, compared with Scrambed control group and showed dose-dependent relation. Signs of apoptosis of K562 cells were not observed. CONCLUSION: Inhibition of K562 cell proliferation, but not cells apoptosis induction is the mechanism of inhibing growth of K562 cells by antisense drug targeted VEGF mRNA. At same time, VEGF has function of promoting K562 cell proliferation, and VEGF mRNA may be a new target attached by drugs.     

不同灌溉施肥方式的土壤硝态氮分布特性试验研究

通过大田测坑玉米种植试验研究,分析了常规畦灌和膜孔灌条件下农田土壤硝态氮的分布特性。结果表明:膜孔灌溉0~60 cm土层的硝态氮在作物的整个生育期变化较大,60 cm以下变化平缓,上下土层硝态氮含量差异相对较小。而常规畦灌在0~40 cm内变化较大,上下土层间硝态氮含量差异较大。两种灌溉方式,表层土壤的硝态氮含量在整个玉米生育期为双峰形式,以三叶期和抽穗期为最高。与常规畦灌相比,膜孔灌溉消除了硝态氮的表聚现象,土壤剖面硝态氮分布更趋合理,更加有利于作物的吸收利用。膜孔灌溉由于其覆膜作用,减轻了硝态氮的淋洗,提高了氮素的利用率。     

膜孔灌灌施条件下硝态氮迁移分布规律研究

利用自行研制的膜孔点源入渗装置,测试膜孔灌灌施条件下硝态氮的迁移和分布规律。结果表明:在肥液连续入渗过程中,硝态氮浓度锋运移与水分湿润锋是一致的;随着距离膜孔中心距离的增加,NO3-N含量减小,在湿润锋位置处土壤NO3-N含量急剧减小到本底值;进入再分布过程后,土壤水分运移速度减慢,整个湿润土体内的含水量分布更加均匀;再分布1~7天内,硝态氮含量略有下降,再分布10天后,硝态氮反硝化作用增强,径向处的反硝化作用弱于垂向处,硝态氮含量明显降低。     

ASP技术在"中国茶主题网关"建设中的应用

介绍了网站信息资源组织的新模式--主题网关的概念与特征;介绍了ASP技术的相关概念;并以中国茶主题网关建设为例,介绍了ASP技术在主题网关建设中的应用.     

灌水定额对波涌灌溉土壤间歇入渗特性的影响

通过肥液(KNO3溶液)室内入渗试验,模拟研究了地下水位埋深150 cm条件下灌水定额对波涌灌溉土壤间歇入渗特性的影响.结果表明,在试验条件下平均入渗速度随灌水定额的增加而减小;灌水定额越大平均周期减渗率越大;土壤含水率增幅随灌水定额的增加而增大;灌水定额越大,土壤水分向下运移越深,进入地下水中的水量越多,越容易造成地下水污染和水资源浪费.     

北方农牧交错带土地利用及其生态背景分析

在遥感和地理信息系统技术支持下,提取年降水量、≥10℃积温、年平均风速、海拔高度、坡度等数据作为生态背景基本要素,基于NOAA/AVHRR影像分类而成的北方农牧交错带全样带1989年和2000年两期土地利用数据进行空间分析,分别研究单一和复合生态背景下土地利用的时空变化特征及其与生态背景的关系。结果表明:年降水量和年平均风速对土地利用变化的影响较为明显,特别是在坡度<15°的区域,降水对耕地和草地、年平均风速对林地和草地的影响均比较明显。≥10℃积温的变化与土地利用关系则不明显。耕地、林地、草地和城镇用地主要分布在海拔1 000~3 500 m和500 m以下、坡度为15°以下的地区,水域主要分布在海拔200 m以下、坡度为5°以下的地区。     

油茶籽成熟过程中油脂及营养物质变化的研究

通过油茶籽主要理化性质和营养成分的分析,研究了油茶籽成熟过程中油脂转化及营养物质的变化规律。结果表明：在油茶籽成熟过程中,鲜籽含水率持续下降,鲜出籽率变化不大,鲜籽出仁率小幅上升,干出籽率和含油率不断上升。油茶籽油中的酸值和过氧化值总体呈下降趋势。在油茶籽成熟过程中,油酸相对含量整体呈上升趋势,亚油酸和亚麻酸相对含量都呈下降趋势,与油酸含量呈极显著负相关（p≤0.01）,相关系数r分别为-0.978和-0.957。油茶籽成熟过程中,油脂积累和可溶性总糖含量的变化呈负相关（r=-0.289）,与可溶性淀粉（r=0.836）和可溶性蛋白（r=0.703）含量变化均呈显著正相关。α-生育酚含量变化基本上是先增加,在9月28日达到最大值0.153 mg·g-1后基本维持稳定,角鲨烯和β-谷甾醇含量随着油茶籽的成熟先增加后下降。     

中籼型水稻两用核不育系佳丰68S的选育及初步利用

佳丰68S是利用"8077S"与丰两优1号杂交,然后再与蜀恢527和扬稻6号复交选育而成的携带苯达松致死基因的中籼型水稻光(温)敏核不育系.它属于无花粉类型,不育期间败育彻底,不育性稳定,其不育株率、花粉不育度和不育株率均为100%,在14.5 h长日下,不育临界温度低于23.5℃.开花习性好,容易繁殖和制种,稻米外观品质好,配合力强.利用该不育系配制的组合正在参加各级产量试验.