轻型木结构建筑覆板的集中静载性能

介绍轻型木结构建筑覆板集中静载结构性能试验的装置、条件和方法,并进行国产人工林落叶松胶合板作轻型木结构覆板的集中静载性能研究.结果表明:与国外胶合板覆板具有相同厚度的国产落叶松屋面板和楼面板,其集中载荷性能均优于相关标准的要求.     

Expression of cyclooxygenase-2 in IL-1β-stimulated mesangial cells is medicated by NF-κB/IκB signal pathway

AIM: To investigate the role of NF-κB/IκB signal pathway in the regulation of cyclooxygenase-2 (COX-2) expression in human mesangial cells (HMC). METHODS: The PGE₂ concentration in supernatants of HMC was measured by radioimmunoassay. COX-2 mRNA and protein expression were determined by RT-PCR and Western blot. Electrophoretic mobility shift assay (EMSA) and Western blot were used to detect the activity of NF-κB and degradation of IκB. RESULTS: IL-1β significantly upregulated COX-2 expression and PGE₂ production in HMC. Significant up-regulation of NF-κB activation, nuclear translocation of p65 subunit, and degradation of IκB α and IκB β were observed in IL-1β-induced HMC. CONCLUSION: Expression of COX-2 in IL-1β-induced HMC is mediated by NF-κB/IκB signal pathway.     

Inhibition of K562 cell growth by antisense drug targeting with VEGF mRNA in vitro

AIM: To investigate inhibition of K562 cell growth by antisense drug targeted VEGF mRNA. METHODS: X7, 20-mer antisense sequences were selected, synthesized and modified with phosphorothioate. The drug was transfected into K562 cells in the present of lipofection. Cell growth was assayed by trypan blue dye exclusion assay and MTT. The level of VEGF protein in the media was determined by ELISA. The morphology of apoptotic cells were observed by Giemsa staining, and the propotion of apoptotic cells was detected by flow cytometry. RESULTS: The antisense drug inhibited growth of K562 and downregulated expression of VEGF protein significantly, compared with Scrambed control group and showed dose-dependent relation. Signs of apoptosis of K562 cells were not observed. CONCLUSION: Inhibition of K562 cell proliferation, but not cells apoptosis induction is the mechanism of inhibing growth of K562 cells by antisense drug targeted VEGF mRNA. At same time, VEGF has function of promoting K562 cell proliferation, and VEGF mRNA may be a new target attached by drugs.     

探索以黄粉虫蛹为食物繁育蠋蝽的可行性

探索以黄粉虫(Tenebrio molitor)蛹为食物进行人工扩繁蠋蝽(Arma chinensis)的可行性,在室内测定比较了蠋蝽分别取食黄粉虫蛹、黏虫(Mythimna separata)蛹和幼虫3种不同食物后的发育历期、死亡率、成虫体重及产卵量等生物学参数。结果表明,蠋蝽取食黄粉虫蛹能正常完成世代发育,其中,取食3种食物的蠋蝽成虫体重无显著性差异。蠋蝽取食黄粉虫蛹,若虫历期为27.97 d,显著低于黏虫蛹组的25.30 d,与黏虫幼虫组31.27 d之间差异显著;若虫期死亡率为26.67%,明显低于取食黏虫蛹和黏虫幼虫的33.33%、83.33%,与黏虫幼虫组之间差异显著;世代单雌平均产卵量为266.67粒,与黏虫蛹组413.00粒之间差异显著,而与黏虫幼虫组292.3粒之间差异不显著。     

我国北方强风蚀区空间分布格局及特征分析

我国北方强风蚀区是形成沙尘天气的主要区域,本文依据各风蚀区风速的大小、走向,自然地理条件,沙化土地现状及分布特点,将我国北方沙区分为古尔班通古特沙漠西北缘、天山东段—塔克拉玛干沙漠南缘、柴达木盆地西段、河西走廊—阿拉善、鄂尔多斯—乌拉特、浑善达克沙地东北部、内蒙古东部七个强风蚀区。同时从沙化土地、降水量、风速及大风日数、沙尘天气的发生等方面对强风蚀区空间分布格局及特征进行了分析。     

单纯性肥胖儿童综合干预及健康教育模式探讨

目的对单纯性肥胖儿童进行综合干预,探讨"医院-学校-社区-家庭四位一体"的健康教育模式,达到对单纯性肥胖儿童的有效控制和良好的健康教育。方法肥胖组采用:(1)干预问卷调查设计方案;(2)对肥胖组儿童的干预;(3)学校与社区对肥胖儿童的发展关注与否的调查与干预;(4)家长对儿童养育观念的影响;(5)测定干预前后的两组生化检测指标(总胆固醇、甘油三酯、血尿酸、血糖);对照组:在未做任何干预的情况下对本组儿童进行相应的问卷调查与生化检测。结果经干预后肥胖儿童吃饭速度快、常吃油炸食品者比干预前明显减少,每天户外活动超过2h和有吃早餐良好习惯者则比干预前明显增多(P<0.01或0.05);肥胖组干预前的总能量、脂肪、总胆固醇和血尿酸均明显高于对照组(P<0.05),干预后肥胖组的能量摄入明显下降(P<0.05),脂肪的摄入及总胆固醇和血尿酸也有所下降,但差异尚未达统计学意义(P>0.05);干预后肥胖组儿童家长对孩子的养育观念更科学、养育方式更为合理。结论实施"医院-学校-社区-家庭四位一体"的健康教育模式,制定综合干预措施,有效降低了单纯肥胖儿童的发生率,对减少肥胖所致的并发症具有重要的临床意义。     

ASP技术在"中国茶主题网关"建设中的应用

介绍了网站信息资源组织的新模式--主题网关的概念与特征;介绍了ASP技术的相关概念;并以中国茶主题网关建设为例,介绍了ASP技术在主题网关建设中的应用.     

灌水定额对波涌灌溉土壤间歇入渗特性的影响

通过肥液(KNO3溶液)室内入渗试验,模拟研究了地下水位埋深150 cm条件下灌水定额对波涌灌溉土壤间歇入渗特性的影响.结果表明,在试验条件下平均入渗速度随灌水定额的增加而减小;灌水定额越大平均周期减渗率越大;土壤含水率增幅随灌水定额的增加而增大;灌水定额越大,土壤水分向下运移越深,进入地下水中的水量越多,越容易造成地下水污染和水资源浪费.     

热带与南亚热带植物生态类型多样性的应用

在适地适树配植的基础上,以光、水和土壤3个自然因素为要素,将数百种热带、南亚热带植物归纳为9个生态类型,以期为种植设计提供参考。     

杜鹃花属植物SRAP-PCR反应体系的优化及其应用

采用改良CTAB法提取杜鹃花属植物基因组DNA,对影响SRAP-PCR反应体系中的Mg2+、dNTPs和引物浓度进行优化,建立了杜鹃花属植物SRAP分子标记的扩增体系:20μl的PCR体系中含有模板DNA50 ng、10×PCR buffer(不含Mg2+)、Mg2+2.50 mmol/L、dNTPs 0.20 mmol/L、引物0.40μmol/L、TaqDNA聚合酶1.0 U。并对10种杜鹃花属植物群体进行扩增验证,共获得261条扩增条带,251个多态性位点,多态性位点比率为96.17%,结果表明供试材料间差异明显、多态性较高,该体系适合杜鹃花属植物种间差异性分析,为今后杜鹃花属植物分子生物学的深入研究奠定了基础。