全文获取类型
收费全文 | 538篇 |
免费 | 54篇 |
专业分类
林业 | 45篇 |
农学 | 26篇 |
基础科学 | 5篇 |
135篇 | |
综合类 | 30篇 |
农作物 | 59篇 |
水产渔业 | 49篇 |
畜牧兽医 | 202篇 |
园艺 | 12篇 |
植物保护 | 29篇 |
出版年
2024年 | 1篇 |
2022年 | 7篇 |
2021年 | 7篇 |
2020年 | 21篇 |
2019年 | 18篇 |
2018年 | 11篇 |
2017年 | 34篇 |
2016年 | 28篇 |
2015年 | 20篇 |
2014年 | 40篇 |
2013年 | 40篇 |
2012年 | 32篇 |
2011年 | 24篇 |
2010年 | 26篇 |
2009年 | 23篇 |
2008年 | 36篇 |
2007年 | 29篇 |
2006年 | 27篇 |
2005年 | 16篇 |
2004年 | 22篇 |
2003年 | 21篇 |
2002年 | 16篇 |
2001年 | 15篇 |
2000年 | 11篇 |
1999年 | 10篇 |
1998年 | 9篇 |
1997年 | 10篇 |
1996年 | 6篇 |
1995年 | 2篇 |
1994年 | 4篇 |
1993年 | 1篇 |
1992年 | 4篇 |
1991年 | 3篇 |
1990年 | 1篇 |
1989年 | 3篇 |
1988年 | 2篇 |
1987年 | 3篇 |
1986年 | 1篇 |
1985年 | 2篇 |
1984年 | 1篇 |
1976年 | 1篇 |
1972年 | 1篇 |
1971年 | 2篇 |
1961年 | 1篇 |
排序方式: 共有592条查询结果,搜索用时 31 毫秒
1.
Mouse pronuclear stage embryos with intact slit zona pellucida (manipulated) were cultured in vitro until the hatched blastocyst stage in simplex optimized medium with higher K+ concentration (KSOM) prepared with three different water types: tap, deionized reverse osmosis (D‐O) water and Milli‐Q system (M‐Q) water. The culture media were supplemented with or without protein and ethylenediaminetetraacetic acid (EDTA, disodium salt). The rates of hatched blastocysts were significantly affected (p < 0.01) by micromanipulation, protein supplement and water source. The water source has no influence (p > 0.05) on development in EDTA‐supplemented protein‐free culture media, whereas in EDTA‐ and protein‐free culture media, the water quality significantly (p < 0.001) affected the rates of development, with higher rates in media prepared with M‐Q water. The micromanipulated embryos showed higher sensitivity to the water quality (p < 0.01). It worth mentioning that the rates of hatched blastocysts in protein‐free culture media were very low (0–7.5%). Furthermore, the three different water types were analysed by measuring the electrical conductivity, inorganic ions, total organic carbon and endotoxins to evaluate the purity. M‐Q water showed the lowest levels of inorganic ion, total organic carbon and endotoxin concentrations. We concluded that manipulated mouse embryos are good system to evaluate the quality of water used in biological system. 相似文献
2.
F.J. López-Escudero C. del Río J.M. Caballero M.A. Blanco-López 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(1):79-85
Resistance of 23 important olive cultivars to Verticillium dahliae has been evaluated in four experiments under controlled conditions. Nine-month-old nursery olive plants were inoculated with a cotton non-defoliating (ND) (V4) or a cotton defoliating (D) (V117) isolate of V. dahliae. Resistance was evaluated by assessing symptom severity using a 0–4 rating scale and estimating the area under disease progress curves. The percentage of plants killed and of those which recovered from the disease were used as additional parameters for including a particular cultivar into a defined category. Most of the evaluated cultivars were susceptible, although at different levels, to both isolates of V. dahliae. All cultivars were more susceptible to the D pathotype than to the ND one. A group of 11 cultivars, including several important Spanish cultivars, were susceptible or extremely susceptible to both pathotypes of V. dahliae. A second group showed differences of resistance depending on the pathotype used. They were susceptible or extremely susceptible to the D pathotype but resistant or moderately susceptible to the ND one. Finally, 'Frantoio', 'Oblonga' and 'Empeltre' were moderately susceptible to the D isolate of V. dahliae and resistant to the ND one. The resistance of 'Empeltre' was evident by the plant ability to recover from infection with either isolates. 'Empeltre' is considered to be a valuable cultivar for inclusion in breeding programmes for resistance to Verticillium wilt. 相似文献
3.
Efficacy of a mephentermine‐based product as a vasopressor and a cardiac performance enhancer when given intramuscularly to cattle
下载免费PDF全文
![点击此处可从《Journal of veterinary pharmacology and therapeutics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
D. N. Q. Cunha C. L. del Rio B. L. Youngblood E. L. Ferris G. E. Geist Y. Ueyama L. C. Franz S. Mathur R. L. Hamlin 《Journal of veterinary pharmacology and therapeutics》2018,41(2):274-280
The goal of this study was to confirm the vasopressor and cardiac effects of POTENAY® INJETÁVEL (POT), a mephentermine‐based product, given to cattle with induced vascular/cardiac depression. Ten healthy Holstein cattle (206 ± 13 kg) followed a randomized‐complete‐block design (RCBD) utilizing crossover study design. Each animal randomly received (1 ml/25 kg, IM) of either POT (n = 10) or volume‐matched placebo control (0.9%NaCl, CP, n = 10). A subset of animals (n = 5) received POT first (day 0) while the remaining (n = 5) received CP; after a six‐day washout period, cattle received the opposite compound. Animals were anesthetized and catheterized for systemic/left ventricular hemodynamic monitoring. Myocardial dysfunction/hypotension was induced by increasing the end‐tidal isoflurane concentration until arterial blood pressure was 20% lower than at baseline and remained stable. Once the animal was determined to be hypotensive and hemodynamically stable, steady‐state hypotensive baseline data (BL2) were acquired, and treatment with either POT or CP was given. Data were acquired post‐treatment at every 15 min for 90 min. POT improved cardiac output (+68 L/min, ±14%, p < 0.05), MAP (+14 mmHg, ±4%, p < 0.05), HR (+22 bpm, ±8%, p < 0.05), and peak rates of ventricular pressure change during both systole (dP/dtmax: +37 mmHg/s ±13%, p < 0.05) and diastole (dP/dtmin: +31 mmHg/s, ±7%, p < 0.05). No improvements were noted following placebo‐control administration. Results indicate that POT improves cardiac performance and systemic hemodynamics in cattle with induced cardiovascular depression when given as single intramuscular injection. 相似文献
4.
ACE inhibition in goats under fixed‐time artificial insemination protocol increases the pregnancy rate and twin births
下载免费PDF全文
![点击此处可从《Reproduction in domestic animals》网站下载免费的PDF全文](/ch/ext_images/free.gif)
VP Fernandes Neto MNN Silva AS Costa D Kunkel AS Júnior LCS Feitosa MCS Muratori APR Costa 《Reproduction in domestic animals》2018,53(4):1006-1008
To assess the effect of the angiotensin‐converting enzyme (ACE) inhibition on the efficiency of the fixed‐time artificial insemination (TAI), 69 goats were divided randomly into two groups: enalapril (n = 35) and control (n = 34). In the experiment, all animals underwent the protocol of fixed‐time artificial insemination for 12 days. Enalapril group received enalapril maleate dissolved in saline (Enalapril, Lab Teuto Ltda) subcutaneously at the following doses: 0.2 mg/kg/day in D0‐D2; 0.3 mg/kg/day in D3‐D6 and 0.4 mg/kg/day in D7‐D11. The control group received the corresponding volume of 0.9% saline solution. We performed a single insemination 36 hr after sponge removal using frozen semen from two adult male goats with recognized fertility. The ultrasound pregnancy diagnosis was 30 days after the artificial insemination (AI). There was significant increase in pregnancy rates and twinning as well as a decrease in foetal loss in animals receiving enalapril (p < .01). The use of ACE inhibitors during the TAI protocol was shown to be a promising alternative to increase the efficiency of such reproductive biotechnology. 相似文献
5.
6.
Nicols Javier Litterio Augusto Matías Lorenzutti María del Pilar Zarazaga Martín Alejandro Himelfarb Manuel Ignacio San Andrs‐Larrea Juan Manuel Serrano‐Rodríguez 《Journal of veterinary pharmacology and therapeutics》2021,44(1):68-78
Cefquinome is a fourth‐generation cephalosporin that is used empirically in goats. Different physiologic factors like pregnancy or lactation could determine the pharmacokinetic behavior of drugs in the organism. The objectives of this study are to (a) compare the pharmacokinetics of cefquinome after intravenous and intramuscular administration in adult nonpregnant (n = 6), pregnant (n = 6), and lactating goats (n = 6), at a dose of 2 mg/kg, with rich sampling by nonlinear mixed‐effects modeling, (b) conduct a pharmacokinetic/pharmacodynamic analysis to evaluate the efficacy of the recommended posology in goats with different physiological states, and (c) determine the optimal posology that achieve a PTA value ≥ 90%, taking into account a T > MIC ≥ 60% of a MIC value ≤ 0.25 µg/ml, in the different subpopulations of goats for both routes. Gestation significantly increased Ka and V1, while reduced F0, Cl, and Q. On the other hand, lactation significantly increased V1 and reduced Tk0. Cefquinome concentrations achieved in placental cotyledon, amniotic fluid, and fetal serum indicate a minimal penetration across the placental barrier. Moreover, milk penetration of cefquinome was minimal. The total body clearance of cefquinome for goats was 0.29 L kg?1 hr?1, that is apparently higher than the reported for cows (0.13 L kg?1 hr?1) and pigs (0.16 L kg?1 hr?1). So, the optimal dose regimen for cefquinome after intravenous and intramuscular administration required higher dose and frequency of administration compared with recommendations for cows or pigs. Therefore, 2 mg kg?1 8 hr?1 and 5 mg kg?1 12 hr?1 could be used for IV and IM routes, respectively, for the treatment of respiratory infections caused by P. multocida and M. haemolytica, but only 5 mg kg?1 12 hr?1 by both routes should be recommended for Escherichia coli infections. 相似文献
7.
The use of midday leaf water potential for scheduling deficit irrigation in vineyards 总被引:1,自引:0,他引:1
J. Girona M. Mata J. del Campo A. Arbonés E. Bartra J. Marsal 《Irrigation Science》2006,24(2):115-127
Midday leaf water potential (Ψmd) was monitored for 3 years at a commercial vineyard (cv. Pinot Noir) under four irrigation strategies. Three treatments were
established based on irrigating vines with 4–6 mm/day, when daily measured Ψmd was more negative than the pre-defined threshold. After the first experimental year, thresholds were adjusted for each treatment
as: (1) Control (C), irrigated when Ψmd was less than −0.6 MPa at the beginning of the season and gradually fell to −0.8 MPa at about mid-June, after which the threshold
was maintained at −0.8 MPa until harvest. (2) Control–Deficit (CD), irrigated as C from bud-break to mid-June (around the
middle of Stage II of fruit growth), and from then until harvest when Ψmd decreased below −1.2 MPa. (3) Deficit–Deficit (DD), irrigated when Ψmd was less than −1.0 from bud break to mid-May (about the middle of fruit growth Stage I), and after that time the Ψmd threshold became −1.2 MPa until harvest. A fourth treatment was applied following a soil water budget approach (WB). All
treatments were replicated five times but irrigation in the Ψmd-based treatments were independently applied to each of the replicate plots, whereas irrigation for WB was applied equally
to all replications. The more site-specific information obtained from Ψmd thresholds in C provided substantial advantages for yield homogeneity and repeatability of results with respect to WB, thus
demonstrating the method’s greater ability to account for spatial variability. Average applied water for the 3 years in C,
CD, and DD was 374, 250, and 178 mm, respectively, while the yields were 11.8, 9.2, and 6.1 kg/vine, respectively. The CD
treatment produced better juice quality than C, and was superior in other quality parameters to both C and DD. However, over
the study period, an important carryover effect was observed in the yields and the grape size of CD, which tended to diminish
from year to year relative to C. 相似文献
8.
Blanch Gracia Patricia Gómez-Jiménez Maria C. del Castillo Maria Luisa Ruiz 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(2):177-183
Plant Foods for Human Nutrition - Grapes contain high contents of phenolics, which are known to possess health promoting properties. Exogenous application of phytoregulators, mainly methyl... 相似文献
9.
Domenico Voltolina María del Pilar Snchez-Saavedra Luz María Torres-Rodríguez 《Aquacultural Engineering》2008,38(2):93-96
The diatom Chaetoceros muelleri was grown in outdoor mass cultures under the winter, spring and summer conditions of Bahia Kino, Sonora, NW Mexico. The solar irradiance in winter was close to 60% of that available in spring and summer, but the cell concentrations and the harvestable biomass were one order of magnitude higher in spring and summer than in winter. There was no difference between the biomass harvested after 2 and 3 days in winter and summer, whereas in spring it was higher after 3 days. The protein content was significantly lower in winter, and carbohydrates and lipids were higher in winter and spring. The number of cells and the amount of harvestable biomass of outdoor cultures of C. muelleri depend on the temperature prevailing in each season, which causes significant differences in growth rates and in biochemical composition. 相似文献
10.
M L Sanz M D del Castillo N Corzo A Olano 《Journal of agricultural and food chemistry》2001,49(11):5228-5231
The presence of Amadori compounds in commercial dehydrated fruits has been shown through HPLC analysis of the corresponding 2-furoylmethyl-amino acids obtained by acid hydrolysis. Furosine (2-furoylmethyl-lysine) was the main 2-furoylmethyl derivative observed in dried figs and apricot samples, whereas in prunes and dates similar amounts of furosine and 2-furoylmethyl-gamma-aminobutyric acid were detected. A considerable variation of 2-furoylmethyl-amino acid contents among commercial raisin samples was observed. 2-Furoylmethyl-gamma-aminobutyric acid and 2-furoylmethyl-arginine, the most abundant 2-furoylmethyl-amino acids, ranged between 9.9 and 75.8 mg/100 g sample and 10.0 and 62.5 mg/100 g sample, respectively. Most of the Amadori compounds present in raisins seem to have originated during the commercial shelf life period rather than during processing. Determination of 2-furoylmethyl-amino acids could be used as a method of controlling commercial dehydrated fruit and selecting storage conditions. 相似文献