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排序方式: 共有122条查询结果,搜索用时 15 毫秒
1.
ABSTRACT: Stranded organisms were observed on the sandy beach of Kuroshima Island, Ryukyu Archipelago, in the early morning of 28 January 1993. Stranded organisms of 525 individuals were classified into 74 species. These organisms included 13 individuals of Panulirus pueruli, which were identical to P. longipes bispinosus. Kuroshima Island is located near Ishigakijima and Iriomote islands, Ryukyu Archipelago, where coral reefs develop. Coral reefs are common and develop in the Ryukyu Archipelago, southern Japan, with the exception of Yakushima and Tanegashima islands. Panulirus japonicus in Japanese waters has not been found in coral reef regions where P. longipes bispinosus are mainly found. The present study describes and identifies to species level puerulus specimens that were stranded on the beach of Kuroshima Island. 相似文献
2.
Burkholderia plantarii , the pathogen of bacterial seedling blight of rice, was detected in paddy water. Its concentration rose in July and August.
The bacterial concentration in the paddy water was always higher along levees than at distances more than 5 m from levees.
Confirmed to be released into water when graminaceous weeds were immersed, B. plantarii survived for at least 4 days at 30°C. B. plantarii was splashed at least 30 cm upward by rain splash in the field. Harvested seeds, which had been sprayed with B. plantarii released from graminaceous weeds at the flowering stage, retained the bacteria. Bacterial seedling blight occurred when the
seeds were then sown in nursery boxes. These results indicated that graminaceous weeds growing on levees of paddy fields are
a source of infection of the disease and that rice seeds are infected through the paddy water.
Received 23 May 2002/ Accepted in revised form 1 September 2002 相似文献
3.
4.
lori INOUE Toshiaki OHARA Fumio NAMIKI Takashi TSUGE 《Journal of General Plant Pathology》2001,67(3):191-199
Restriction enzyme-mediated integration (REMI) mutagenesis was used to isolate mutants of Fusarium oxysporum f. sp. melonis impaired in pathogenicity. The race 2 strain Mel02010 was transformed with linearized pSH75, conferring resistance to hygromycin
B, with or without the enzyme used to linearize the plasmid. Addition of restriction enzymes did not affect the transformation
frequency. A total of 2929 REMI transformants were tested for pathogenicity to three melon cultivars, Amus, Ogon 9 and Ohi.
The race 2 strains are pathogenic to Amus and Ogon 9, but not to Ohi. Of 43 transformants with reduced pathogenicity on susceptible
melon cultivars, 12 mutants were examined in detail for pathogenicity, vegetative growth and integrative mode of pSH75. The
levels of pathogenicity varied among these mutants. Two mutants (B48 and B137) almost completely lost pathogenicity to both
susceptible cultivars, and the others had reduced pathogenicity. Mutants B48, B241, B886 and X36 were also impaired in vegetative
growth. Mutant B809 was a biotin auxotroph. By DNA gel blot analysis, nine mutants were found to contain a single copy of
the transformation vector. These mutants may thus be useful in isolating genes involved in pathogenicity.
Received 22 December 2000/ Accepted in revised form 16 April 2001 相似文献
5.
Ehab MOSSAAD Masahito ASADA Daichi NAKATANI Noboru INOUE Naoaki YOKOYAMA Osamu KANEKO Shin-ichiro KAWAZU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):53-58
Bovine babesiosis is a livestock disease known to cause economic losses in
endemic areas. The apicomplexan parasite Babesia bovis is able to invade
and destroy the host’s erythrocytes leading to the serious pathologies of the disease,
such as anemia and hemoglobinuria. Understanding the egress mechanisms of this parasite is
therefore a key step to develop new therapeutic strategies. In this study, the possible
involvement of Ca2+ in the egress of B. bovis merozoites from
infected erythrocytes was investigated. Egress was artificially induced in
vitro using calcium ionophore A23187 and thapsigargin to increase
Ca2+ concentration in the cytosol of the parasite cells. The increased
intracellular Ca2+ concentration following these treatments was confirmed using
live cell Ca2+ imaging with confocal laser scanning microscopy. Based on our
findings, we suggest a Ca2+ signalling pathway in the egress of B.
bovis merozoites. 相似文献
6.
Sadamasa ISHIKAWA Kou HIRAGA Yuuki HIRADATE Kentaro TANEMURA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):725-728
Acetamiprid (ACE) and imidacroprid (IMI) are known neonicotinoid insecticides with strong
affinities for the insect-selective nicotinic acetylcholine receptor. These provide insect
control by hyperstimulating insect nerves and are used for agricultural pest management.
However, it has also been reported that ACE and IMI affect mammalian reproductive
function. We determined the effects of ACE and IMI on the in vitro
maturation of porcine oocytes. Significant decreases in nuclear maturation rates were
observed in the ACE or IMI-exposed groups. Also, in matured oocytes from the ACE or
IMI-exposed groups, irregular chromosomes were observed. Our results suggest that ACE and
IMI exposure was detrimental to porcine oocytes and the extent of the effects depends on
the concentration of exposure. 相似文献
7.
Weak activity of UDP-glucuronosyltransferase toward Bisphenol analogs in
mouse perinatal development
Risa YABUSAKI Hidetomo IWANO Sumito TSUSHIMA Nanako KOIKE Naoko OHTANI Kentaro TANEMURA Hiroki INOUE Hiroshi YOKOTA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1479-1484
Bisphenol A (BPA) is a widely used industrial chemical that disrupts endocrine function.
BPA is an endocrine disrupting chemical (EDC) that has been demonstrated to affect
reproductive organ development, brain development, metabolic disease and post-natal
behavior. Accordingly, Bisphenol analogs, Bisphenol F (BPF, bis (4-hydroxyphenyl) methane)
and Bisphenol AF (BPAF, 4,4-hexafluoroisopropylidene) diphenol) are used as replacements
for BPA. BPA is mainly metabolized by UDP-glucuronosyltransferase (UGT), UGT2B1, but this
effective metabolizing system is weak in the fetus. In the present study, we demonstrated
that hepatic UGT activity toward BPAF was very weak, in comparison with BPA and BPF, in
the fetus, pups and dams. Conversely, hepatic UGT activity toward BPF was very weak in the
fetus and newborn pups, and was increased to the same level as BPA post-partum. In
conclusion, BPAF possibly tends to accumulate in the fetus, because of weak metabolism
during the perinatal period, suggesting that the metabolism of individual Bisphenol
analogs requires assessment to properly gauge their risks. 相似文献
8.
Atsushi IDETA Yoshito AOYAGI Kanami TSUCHIYA Yuuki NAKAMURA Kou HAYAMA Atsushi SHIRASAWA Kenichiro SAKAGUCHI Naomi TOMINAGA Yoshiyuki NISHIMIYA Sakae TSUDA 《The Journal of reproduction and development》2015,61(1):1-6
Embryos obtained via superovulation are necessary for mammalian artificial reproduction, and viability is a key determinant of success. Nonfreezing storage at 4 C is possible, but currently used storage solutions can maintain embryo viability for only 24–48 h. Here we found that 10 mg/ml antifreeze protein (AFP) dissolved in culture medium 199 with 20% (v/v) fetal bovine serum and 25 mM HEPES could keep bovine embryos alive for 10 days at 4 C. We used a recombinant AFP isolated from the notched-fin eelpout (Zoarces elongatus Kner). Photomicroscopy indicated that the AFP–embryo interaction was enhanced at 37 C. Embryos pre-warmed with the AFP solution at 37 C for 60 min maintained high viability, whereas those that were not pre-warmed could live no longer than 7 days. Thus, short-term storage of bovine embryos was achieved by a combination of AFP-containing medium and controlled pre-warming. 相似文献
9.
Yuuki Kawabata Junichi Okuyama Hiromichi Mitamura Kimio Asami Kenzo Yoseda Nobuaki Arai 《Fisheries Science》2007,73(5):1147-1154
ABSTRACT: Post-release movement and diel activity patterns of hatchery-reared and wild black-spot tuskfish were examined using ultrasonic telemetry. Five hatchery-reared and four wild fish were released in the sandy bottom of Urasoko Bay, Ishigaki Island, Okinawa, Japan, and monitored using automated monitoring receivers from November 2005 to February 2006. Both hatchery-reared and wild fish tended to stay near the release site for over two weeks, before leaving the release site. Both hatchery-reared and wild tuskfish showed diurnal rhythm intermittently; signals were recorded more frequently in the daytime and less frequently in the nighttime, suggesting that the fish of both origins were active during the day and inactive during the night. These findings indicate that the one-year-old hatchery-reared tuskfish have some consistent behavioral characteristics with those of the wild. 相似文献
10.