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排序方式: 共有289条查询结果,搜索用时 15 毫秒
1.
Kunio Shirakihara Masahiko Yoshida Machiko Nishino Yoshimi Takao Kouichi Sawada 《Fisheries Science》2001,67(3):430-435
SUMMARY: The vertical distribution of dwarf ayu Plecoglossus altivelis altivelis in the pelagic waters of Lake Biwa was evaluated from June to September in 1995–97 from eight acoustic surveys using a quantitative echosounder. In each survey, echoes from a depth range of 3 m to the sea bottom were collected at a station every 2.7 s for 24 h together with measurements of vertical profiles of water temperature and chlorophyll-a. The ayu's echoes were identified using an underwater video camera. The ayu were observed near the maximum chlorophyll-a depth and above the thermocline. Their density was highest at depths of 4–11 m with 2–4 individuals/m 3 and was almost zero below 20 m. Echo signs were recorded as having a frequent duration of more than 1 h at night, whereas were of a shorter duration in the day. The fish stay in the epilimnion during the day without any clear vertical migration, but are distributed more uniformly at night. The advantages of remaining in the epilimnion are discussed in terms of food availability and predator avoidance. 相似文献
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Md Shafiqul ISLAM Urara SHINYA Mitsuhiro TAKAGI Takao AKAHOSHI Akira YABUKI Shahnaj PERVIN Tofazzal Md RAKIB Mohammad Mahbubur RAHMAN Martia Rani TACHARINA Osamu YAMATO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(2):254
Bovine isoleucyl-tRNA synthetase (IARS) disorder, a major cause of weak calf syndrome, is caused by a homozygous missense (c.235G>C) mutation in the bovine IARS gene of Japanese Black (JB) cattle, which was identified in 2013. However, the extent to which the carrier rate has changed at Kagoshima prefecture, Japan, and whether the carrier status is associated with any clinical or reproductive problems, have yet to be ascertained. In this study, using a real-time polymerase chain reaction-based genotyping assay, we determined the carrier rate in a regional JB cow population at Kagoshima prefecture. Comparative analyses were performed on the metabolic profile test (MPT) results and reproductive performance data obtained for heterozygous carrier and homozygous wild-type cows. In 2009 and 2018, DNA samples were collected from 130 and 462 clinically healthy JB cows, respectively, in Kagoshima prefecture. MPT results and reproductive performance data were evaluated for 62 cows, comprising four heterozygous carriers and 58 wild-type cows. Genotyping revealed that the carrier rate was 6.9% in 2009 and 1.5% in 2018, the difference of which was statistically significant (P<0.005). There were no statistically significant differences between the carrier and wild-type cows with respect to either MPT results or reproductive performance, indicating that the carrier cows have necessary IARS activity to maintain minimal health and reproductive potential. 相似文献
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Lactobacillus rhamnosus GG SpaC pilin subunit binds to the carbohydrate moieties of intestinal glycoconjugates
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Keita Nishiyama Shintaro Ueno Makoto Sugiyama Yuji Yamamoto Takao Mukai 《Animal Science Journal》2016,87(6):809-815
Lactobacillus rhamnosus GG (LGG) is a well‐established probiotic strain. The beneficial properties of this strain are partially dependent on its prolonged residence in the gastrointestinal tract, and are likely influenced by its adhesion to the intestinal mucosa. The pilin SpaC subunit, located within the Spa pili structure, is the most well studied LGG adhesion factor. However, the binding epitopes of SpaC remain largely unknown. The aim of this study was to evaluate the binding properties of SpaC to the carbohydrate moieties of intestinal glycoconjugates using a recombinant SpaC protein. In a competitive enzyme‐linked immunosorbent assay, SpaC binding was markedly reduced by addition of purified mucin and the mucin oligosaccharide fraction. Histochemical staining revealed that the binding of SpaC was drastically reduced by periodic acid treatment. Moreover, in the surface plasmon resonance‐based Biacore assay, SpaC bound strongly to the carbohydrate moieties containing β‐galactoside at the non‐reducing terminus of glycolipids. We here provide the first demonstration that SpaC binds to the oligosaccharide chains of mucins, and that the carbohydrate moieties containing β‐galactoside at the non‐reducing termini of glycoconjugates play a crucial role in this binding. Our results demonstrate the importance of carbohydrates of SpaC for mucus interactions. 相似文献
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The success of rapidly growing aquaculture industry depends on the steady supply of fish seeds. Appropriate food for initial larval stages is critical for mass scale fish seed production. Live food found better over artificial food for fish larvae culture. Rotifers have been found to be the best live food for feeding fish larvae in early life stage. Attempts have been made to develop viable techniques for the production of rotifer through batch, continuous, and semi-continuous culture methods. In order to feed increased number of fish larvae, rotifer need to be cultured under high-density method. Various efforts have been made for increasing culture density of rotifer. In Japan, stable high-density culture of rotifer has been developed in commercial scale at a rate of 20,000–30,000 ind. ml?1. Later on, ultra-high-density rotifer culture (160,000 ind. ml?1) was found successful, which can fulfill the increased demand for rotifer as fish larval food. Furthermore, a scope of alternative use of rotifer can be explored. The development of the high-density rotifer culture method in Japan has been reviewed. The considerations of the associated nutritional requirements, microbial aspects, and prospects of high-density culture have been discussed. 相似文献
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Mari ISHIDA Chiho KANEKO Takao IRIE Yoshino MARUYAMA Asami TOKUDA Ayako YOSHIDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(3):454
Paragonimiasis is a zoonotic trematode infection caused by Paragonimus spp. To determine the recent status of Paragonimus infections in wild animals, this study investigated Paragonimus spp. in 39 raccoon dogs and 54 Japanese badgers from March 2019 to January 2021 in Miyazaki Prefecture, and examined metacercariae in freshwater crabs. Triploid P. westermani was found in one raccoon dog (2.6%), and metacercariae were recovered from Eriocheir japonica captured near the infected animal collected. One Japanese badger (1.9%) harbored P. skrjabini miyazakii; this prevalence was lower than the approximately 30% that was reported in the 1970s. Results indicated that zoonotic Paragonimus was sporadically prevalent in wild animals. Further investigation in various animals is awaited to elucidate current wildlife reservoirs for those Paragonimus. 相似文献
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考虑可控非可控因素动态特性的含水层系统有限元模拟模型 总被引:1,自引:0,他引:1
本文提出了考虑可控非可控因素动态特征的水资源系统有限元动态模拟模型,将各单元逐时段系统输入输出信息的动态计算模型引入到模型中,然后由于这些量的动态特性,加之需要模拟识别的参数繁多,给有限元模拟模型的求解工作增加了很大的难度,我们在分析各类参数及曲线精度及物理概念的基础上,先固定一部分参数和曲线,然后从剩余参数的物理概念出发,确定其初值及其相互间的逻辑关系,最后由有限元模拟模型识别出这些参数,通过验证表明了模拟参数的准确性,也充分说明了有限元各项项信息量确定的准确,系统输入输出信息量计算模型合理可靠。 相似文献
10.
Most scientific attention on port studies centers on deep sea ports, especially container ports. In this paper, in contrast, attention is focused on the spatial–temporal development of inland waterway ports on the Midstream Yangtze River from 2001 to 2013. The aim of this study is to assess two relevant and complementary questions of the hinterland evolution: its geographical extent and the coordination relationship with the inland port. To conduct the study, it was necessary to first identify the boundaries of the ports' hinterlands within the given timeframe. Then, the coupling coordination degree model was introduced to explore the underlying relationship between the port service and hinterland economy. Furthermore, to better depict the intricate economic characteristics of the hinterland, the development stage theory was applied in the models. The results highlight the emergence of a discontinuous hinterland at Wuhan Port and its reinforcement of primacy with respect to fierce hinterland rivalry. It also demonstrates that an interplay between major ports and their corresponding hinterlands evolves from the transitional stage, characterized by lagging port service, to the multi‐stage, wherein the supply of ports partly outstrips hinterland demand. 相似文献