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排序方式: 共有116条查询结果,搜索用时 31 毫秒
1.
2.
Yohei Kato 《Pesticide biochemistry and physiology》2004,79(2):64-73
A hydrophilic form of acetylcholinesterase (AChE) was purified from N-methyl carbamate susceptible (SA) and highly N-methyl carbamate-resistant (N3D) strains of the green rice leafhopper (GRLH), Nephotettix cincticeps Uhler. Both of purified AChE from SA and N3D strains displayed the highest activities toward acetylthiocholine (ATCh) at pH 8.5. In the SA strain, the optimum concentrations for ATCh, propionylthiocholine (PTCh), and butyrylthiocholine (BTCh) were about 1 × 10−3, 2.5 × 10−3, and 1 × 10−3 M, respectively. However, in the N3D strain, substrate inhibition was not identified for ATCh, PTCh, and BTCh to 1 × 10−2 M. The Km value in the SA strain was 51.1, 39.1, and 41.6 μM and that in the N3D strain was 91.8, 88.1, and 85.2 μM for ATCh, PTCh, and BTCh, respectively. The Km value in the N3D strain indicated about 1.80-, 2.25-, and 2.05-fold lower affinity than that of the SA strain for ATCh, PTCh, and BTCh, respectively. The Vmax value in the SA strain was 70.2, 30.5, and 4.6 U/mg protein and that in the N3D strain was 123.0, 27.0, and 14.5 U/mg protein for ATCh, PTCh, and BTCh, respectively. The Vmax value in the N3D strain was 1.75- and 3.15-fold higher for ATCh and BTCh than that in the N3D strain. However, it was 1.13-fold lower for PTCh. The increased activity of AChE in the N3D strain is due to the qualitatively modified enzyme with a higher catalytic efficiency. The bimolecular rate constant (ki) for propoxur was 27.1 × 104 and 0.51 × 104 M−1 min−1 in the SA and N3D strain and that for monocrotophos was 0.031 × 104 and 2.0 × 104 M−1 min−1 in the SA and N3D strain. AChE from the N3D strain was 53-fold less sensitive than SA strain to inhibition by propoxur. In contrast, AChE from the N3D strain was 65-fold more sensitive to inhibition by monocrotophos than AChE from the SA strain. This indicated negatively correlated cross-insensitivity of AChE to propoxur and monocrotophos. 相似文献
3.
Hieu Van DONG Maya SUZUKI Hitoshi TAKEMAE Dulamjav JAMSRANSUREN Sachiko MATSUDA Hiep Dinh NGUYEN Tetsuya MIZUTANI Yohei TAKEDA Haruko OGAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(11):1543
In this study, the viral genome extraction performance of automatic nucleic acid extractors and manual nucleic acid extraction kits was compared. We showed that compared with manual kits, the automatic extractors showed superior genome extraction performance using bovine viral diarrhea virus (BVDV) genome-positive cattle sera and bovine coronavirus/infectious bovine rhinotracheitis virus-spiked cattle nasal swabs. In addition, the subgenotyping of BVDV strains detected in Tokachi Province in Japan during 2016–2017 was performed. Results showed that most of these BVDV strains belonged to subgenotype 1b, while few strains belonged to subgenotypes 1a and 2a. This study showed the high applicability of automatic nucleic acid extractors in extracting multiple viral genomes and the dominant subgenotype of BVDV in Tokachi. 相似文献
4.
Hisayuki Arakawa Yohei Ohi Akira Matsumoto Kenji Takio Takumi Kimoto Katsutoshi Kawabe 《Fisheries Science》2012,78(5):1083-1090
The populations of red alga Gelidium elegans along the coast of Miyakejima Island were severely damaged by a volcanic eruption in 2000. The effect of this volcanic eruption has been long lasting, and populations of this red alga still have not recovered. We investigated the effect of seabed sediment particles derived from volcanic ash on the substrate adhesion of G.?elegans spores. The analysis provides evidence that increasing amounts of sediment particles result in lower adhesion rates of G.?elegans spores, and that smaller sediment particles have a greater influence on adhesion. The amount of seabed sediment particles around Miyakejima Island was 9.3?C1815.4?mg/cm2. This amount has changed greatly from year to year. The adhesion rate of G.?elegans spores in water around Miyakejima Island was 0?% at all points in 2008 and 2010, but it was estimated as 6.3?C38.6?% in 2009. These results suggest that there is significant inhibition of algal spore adhesion by seabed sediment particles derived from volcanic ash around Miyakejima Island. 相似文献
5.
Yohei Kawasaki Ryo Yamazaki Katsuyuki Katayama Tetsuya Yamada Hideyuki Funatsuki 《Plant Production Science》2013,16(4):387-397
ABSTRACTUnderstanding how maturity genes affect soybean yield formation will provide important information for crop management decisions. This study aimed to reveal how maturity genes E2 and E3 in the soybean cultivar ‘Enrei’ affect yields and yield formation in warm regions of Japan. ‘Enrei’ (e2e3) and three near-isogenic lines of ‘Enrei’ (e2E3, E2e3, and E2E3) were cultivated in 2016 and 2017 in Fukuyama, Japan (34°30′N, 133°23′E). Two sowing dates were set in each year (June sowing and July sowing). E2 extended the period from emergence to R1 and also the period from R1 to R7, whereas E3 extended only the period from emergence to R1. Interaction between E2 and E3 did not affect duration of the period from emergence to R1, but did affect the period from R1 to R7. Although seed yield did not differ between genotypes in the June sowings, the effects of E2 and E3 on seed yield in July sowing were both significant and interaction between E2 and E3 also observed. The total number of nodes increased in E3 genotypes in both sowing dates, especially in E2E3. Pod-set ratio was lower in E2 and E3 genotypes than in e2 and e3 genotypes in the June sowings, but did not differ between genotypes in the July sowings. The high yield of E2E3 genotypes in the July sowings was attributed to increased number of nodes and flower production while maintaining pod-set ratio. Appropriate choice of sowing date is suggested to be essential when using E3 genotypes.Abbreviations: HI: harvest index; NIL: near-isogenic line; RUE: radiation use efficiency; TDM: total above-ground dry matter; TRI: total solar radiation intercepted 相似文献
6.
Yohei Nakamura Masahiro Horinouchi Mitsuhiko Sano Takuro Shibuno 《Fisheries Science》2009,75(6):1401-1408
Whilst the importance of seagrass beds as nurseries for coral reef fishes has been clearly recognized, the vast majority of
early studies on fish nursery habitats emphasized the close proximity of the latter to coral reefs. To determine the potential
nursery role of isolated seagrass beds, we investigated the degree to which juvenile emperor fishes (Lethrinidae) utilized
seagrass beds in the presence/absence of adjacent coral habitats at Ishigaki Island (southern Japan), such fishes being known
to use seagrass beds as nurseries. Seagrass beds in close proximity to coral habitats (distance between the two habitats of
50–200 m) had greater densities of lethrinid juveniles than those without adjacent coral habitats (2.5–4 km) for 3 different
sites investigated, although a significant difference was obtained only for 1 site. Juveniles of Lethrinus atkinsoni, L. obsoletus, L. harak, and L. nebulosus were observed in seagrass beds with and without adjacent coral habitats, whereas L. ornatus occurred only in the former. Overall, most lethrinid juveniles utilize seagrass beds irrespective of the presence of adjacent
adult coral habitat, suggesting that both types of seagrass beds would contribute to lethrinid adult populations. Therefore,
management efforts for lethrinid populations should be applied not only to contiguous coral-seagrass habitat systems but also
to isolated habitats. 相似文献
7.
Real‐time polymerase chain reaction (PCR) assays for 11 representative rumen bacterial species were validated. The sensitivity was tested by using the serially diluted target 16S rDNA from respective bacterial species. The recovery of the target DNA and the assay reproducibility were determined using DNA from rumen fluid spiked with different quantities of the target. Minimum detection levels for the target were 10–100 copies in pure culture. The recovery of the added target ranged from 82.4 to 116.6%. The intra‐ and inter‐assay variations of each assay were <9.4 and <12.6%, respectively. Therefore, the real‐time PCR assays evaluated in the present study are considered to be sufficiently reliable for monitoring all 11 bacterial species in the rumen. The assays were then applied to the monitoring of the bacterial species attached to ruminally incubated rice straw. Among the monitored fibrolytic species, Fibrobacter succinogenes was found to be the most dominant, accounting for 2.61% of total bacteria after 24 h incubation. Selenomonas ruminantium and Streptococcus bovis, non‐fibrolytics, were detected on the rice straw at 8.96% and 1.16% of total bacteria, respectively. Such high levels of non‐fibrolytics on the plant fiber suggest a synergistic relationship between fibrolytics and non‐fibrolytics. 相似文献
8.
依据林下多年生草本植物山酢浆草Oxalis griffithii的形态学特征,将其生活史划分为4个阶段,并根据3种生境下山酢浆草种群的野外调查资料建立了不同种群的Lefkovitch结构矩阵模型,在此基础上预测了山酢浆草种群的动态及其阶段结构的变化;同时,运用敏感度、贡献率指标分析了影响种群动态的关键生活史阶段。结果表明:不同生境下山酢浆草种群的动态发展趋势不尽相同,常绿阔叶林下的种群(MD)呈稳定发展状态,其他2种生境下种群表现出衰退的发展趋势;种群的动态变化过程受个体的生长、残存、萎缩和繁殖等的综合影响;不同生活史阶段对种群动态的影响差异显著,种群动态过程及其发展趋势主要受种群内较大阶段个体的影响。图2表5参19 相似文献
9.
Weak activity of UDP-glucuronosyltransferase toward Bisphenol analogs in
mouse perinatal development
Risa YABUSAKI Hidetomo IWANO Sumito TSUSHIMA Nanako KOIKE Naoko OHTANI Kentaro TANEMURA Hiroki INOUE Hiroshi YOKOTA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1479-1484
Bisphenol A (BPA) is a widely used industrial chemical that disrupts endocrine function.
BPA is an endocrine disrupting chemical (EDC) that has been demonstrated to affect
reproductive organ development, brain development, metabolic disease and post-natal
behavior. Accordingly, Bisphenol analogs, Bisphenol F (BPF, bis (4-hydroxyphenyl) methane)
and Bisphenol AF (BPAF, 4,4-hexafluoroisopropylidene) diphenol) are used as replacements
for BPA. BPA is mainly metabolized by UDP-glucuronosyltransferase (UGT), UGT2B1, but this
effective metabolizing system is weak in the fetus. In the present study, we demonstrated
that hepatic UGT activity toward BPAF was very weak, in comparison with BPA and BPF, in
the fetus, pups and dams. Conversely, hepatic UGT activity toward BPF was very weak in the
fetus and newborn pups, and was increased to the same level as BPA post-partum. In
conclusion, BPAF possibly tends to accumulate in the fetus, because of weak metabolism
during the perinatal period, suggesting that the metabolism of individual Bisphenol
analogs requires assessment to properly gauge their risks. 相似文献
10.