Summertime and early autumn activity of some enzymes in the carbohydrate and fatty acid metabolism of the crucian carp

In June, July, and September the activities of five enzymes involved in the carbohydrate and fatty acid metabolism, namely phosphofructokinase (PFK), lactate dehydrogenase (LDH), citrate synthase (CS), cytochrome oxidase (cyt ox) and 3-hydroxy-acyl-CoA dehydrogenase (HAD), were measured in the heart, liver, red muscle, white muscle, and gill lamellae of crucian carp (Carassius carassius). LDH activity was measured in both reducing (LDHr) and oxidizing (LDHo) directions.The PFK activity in red and white muscle increased significantly between July and September indicating a preparation to winter anoxia by an increased glycylytic capacity in these organs. The HAD activity of the liver had increased significantly (by more than 50%) by September, also a preparation to winter anoxia as HAD is used in the reversed -oxidation (chain elongation of fatty acids). The LDHr and cyt ox activities in the heart and white muscle were highest in July. This shows that both the anaerobic and aerobic capacities are elevated in mid-summer when water temperature is high and oxygen concentration of the water could fluctuate greatly. The LDHo and CS activities in gill lamellae were lowest in July. The results show that the metabolism of crucian carp is under seasonal influence and that a preparation to winter hypoxia/anoxia could be detected in September.     

Calcification of the intervertebral discs and curvature of the radius and ulna: a radiographic survey of Finnish miniature dachshunds

The vertebral column of 124 randomly selected miniature dachshunds, representing 4.5% of the population registered by the Finnish Kennel Club during the years 1988 to 1996, were radiographed. The front legs were also radiographed in order to evaluate the curvature of the radius and ulna. Calcified discs were found in 75.9% of the longhaired miniature dachshunds and in 86.7% of the wirehaired ones. The occurrence of signs associated with IDD was 16.5% in longhaired and 15.6% in wirehaired miniature dachshunds. The occurrence of signs of IDD in dogs with calcified discs was 20.0% and 17.9% in longhaired and wirehaired miniature dachshunds, respectively. In dogs without calcifications only one dog showed signs of IDD. The curvature of the radius and the ulna did not differ between the dogs with signs of IDD and the healthy ones, or between the dogs with and without intervertebral calcifications. Our results indicate that radiographic eradication based on the presence of intervertebral calcifications is not suitable for breeding purposes for the Finnish miniature dachshund population because the percentage of dogs without calcifications is small.     

Transverse shrinkage in G-fibers as a function of cell wall layering and growth strain

Transverse drying shrinkage was measured at microscopic and mesoscopic levels in poplar wood characterised by an increasing growth strain (GS), from normal to tension wood. Results show that: (a) the drying shrinkage, measured as a relative thickness decrease, was significantly higher for G-layer (GL) than for the other layers (OL), GL shrinkage was not significantly correlated with GS, and OL shrinkage was negatively correlated with GS. (b) In gelatinous fibre (G-fibre), lumen size increased during drying and this increase was positively related with GS, but in normal wood fibre, lumen size decreased during drying. These findings suggest that GL shrank outwards (i.e., its internal perimeter increases), so that its shrinkage weakly affected the total cell shrinkage and the mesoscopic shrinkage was controlled by the OL shrinkage which shrank inwards (i.e., its external perimeter decreases). (c) Measurements done on 7 × 7 mm2 thin sections evidenced a negative correlation between transverse shrinkage and GS, significant in T direction but weak in R direction. These observations at both levels allow to discuss the contribution of GL to the mesoscopic shrinkage of tension wood.     

European restructuring and changing agricultural policies. Rural self-identity and modes of life in late modernity

The main idea of this article is to present various perspectives in order to analyze the recent crisis concerning the agriculture-based rural societies in the developed capitalist communities. In all of these countries there is a production crisis, resulting in too much food. But this is also an ideological crisis, because the consumer thinks that the food is produced at too high a price. And it is a political crisis as well because a major part of the voters think subsidies and trade barriers are too high. The paper argues that beneath the present agricultural and rural policy crisis lies the failure of three great projects of our time: 1) The project of natural science; 2) The project of liberal capitalism; and 3) The project of scientific socialism. The failure of these three projects has to do with the breakdown of the positivist idea of modernization. Modernization theory was partly wrong because it overlooked the persistence of locally based life modes. Those life modes must be understood before a sustainable rural development is found. The article reviews some contemporary social science perspectives that have recently been developed to grasp the fundamental changes of today's rural societies. Based on those perspectives and primarily the life mode perspective, five key elements that are essential to analyze if we want to understand future development are isolated: food production, resources, space, social diversity, and culture.Reidar Almås is a Professor of Sociology at the University of Trondheim, Norway, where he directs the Center for Rural Research. He received his Dr. philos. at the University of Trondheim with a dissertation on group farms. Almås is now doing research on food policies, rural development, and public perception of biotechnology. In addition to several books in Norwegian, he has publishedRural Norway, a gift to Europe. Fifteen selected articles on rural persistence and change.     

Characteristics and conflicts in Norwegian agriculture

This article raises the issue of the extent to which a single nation can develop a “national agricultural policy,” pursuing internal goals in agrarian development, goals that vary significantly from those of other industrialized countries. What are the conflicts arising from such a policy and how do these conflicts interfere with the general agricultural crisis of these countries? The Norwegian case is explored as an example of a blend of social-democratic and center-populist agricultural policies. The decision in 1975 by the Norwegian Parliament to establish the income level of farmers at the same level as workers is seen as an experiment. As agricultural policy, this new departure represented a deviation from the earlier policies of rationalization. The Social Democrats developed their new position as a result of internal and external pressure. A series of reforms financed by the new state incomes from oil production were undertaken, of which income equalization and a farm relief service were the most important. Most Norwegian farmers speak of the reform years as “the good years of escalation.” Equity was reached in 1982, but nevertheless several problems emerged: 1) the escalation of subsidized incomes led to overproduction and decreasing incomes from the market (after 1982), 2) the large budgetary transfers to agriculture caused a legitimation crisis, fueling the constituency of the right-wing Progress Party, 3) the large subsidies to agriculture contributed to the fiscal crisis of the state, 4) the problems mentioned above, contributed to the general crisis of the Norwegian Social Democracy which finds itself with dwindling support. The likely outcome of the present Norwegian farm crisis is the implementation of an agricultural policy that will protect the most vulnerable sector and open the more competitive sector to market forces.     

Appearance of interferon-alpha in serum and signs of reduced immune function in pigs after transport and installation in a fattening farm

Pigs were transported from several breeding facilities at the age of 10-12 weeks and regrouped in a fattening farm, specialized in breeding pigs for subsequent slaughter. Blood samples were obtained from the animals just before transport and daily for 17 days after installation in the fattening farm. On each occasion a group of ten animals (170 animals in total) was sampled. The levels of interferon-alpha (IFN-alpha) in serum were measured as antiviral activity in a cytopathic effect inhibition assay. Beginning at day 4 after installation, a significant proportion of sera contained IFN-alpha, with the highest incidence of IFN-alpha positive animals (25%) and IFN-alpha titers on days 5-10. This indicates a high frequency of viral infections in the animals. The in vitro ability of peripheral blood mononuclear leukocytes (PBMCs) to produce IFN-alpha after stimulation by glutaraldehyde-fixed pseudorabies virus-infected PK15 cells and their proliferative response to the T-cell mitogen leukoagglutinin (LA) was also monitored. There was a significant, but moderate decrease in the ability of PBMCs to produce IFN-alpha during the observation period. In contrast, the response to the mitogen LA decreased markedly during the first 5 days, and thereafter remained at the same low level. The proliferative response to LA was significantly lower for PBMCs from serum of IFN-alpha-positive than from IFN-alpha-negative animals. These impaired PBMC responses could indicate a stress-induced immune depression, possibly contributing to the high incidence of viral infections.     

Lymphokines in the regulation of immune responses

Lymphokines are polypeptide factors produced by lymphocytes and other leukocytes, which modulate leukocyte activities in immune responses. Some of the more important lymphokines are reviewed in detail, with emphasis on those that have been cloned. These include interferon -alpha and -beta, interferon gamma, interleukin 2, interleukin 1, tumor necrosis factor and lymphotoxin, B lymphocyte growth factors, hemopoietic growth factors, and others.