Purification, serology and properties of five zucchini yellow mosaic virus isolates

Five zucchini yellow mosaic virus (ZYMV) isolates designated ZYMV-1, -3, -5, -7 and -FL were purified from zucchini plants grown under the same environmental conditions. Isolates were divided into three groups on the basis of yield of purified virus: group one (ZYM V-5 and -7) yielded 18-24 mg/100 g tissue, group two (ZYMV-3) 5.0-5.7 mg/100 g tissue, and group three (ZYMV-1 and -FL) low yields of 0-5-1-5 mg/100 g tissue. The yield of purified virus was positively correlated with the number of local lesions produced by inoculation of Chenopodium amaranticolor with extracts from infected zucchini leaf tissue. No serological differences were exhibited among the isolates in SDS-immunodiffusion tests. Results of ELISA tests indicated that ZYMV-FL differed from the other isolates and that antiserum to ZYMV-7 absorbed with ZYMV-FL failed to react with ZYMV-FL but retained some serological activity to ZYMV-7 and the other three Taiwanese isolates. The relative molecular masses of capsid proteins for the four ZYMV isolates from Taiwan and ZYMV-FL were similar.     

Effect of different sources of dietary fibre on growth performance,intestinal morphology and caecal carbohydrases of domestic geese

1. The aim of this study was to examine the effects of dietary fibre sources on growth and on the development of the gastro-intestinal tract in growing geese. 2. Six-week-old female White Roman geese were divided at random into 6 groups with 6 dietary treatments. Diets were isoenergetic and isonitrogenous and contained alfalfa meal, barley bran, rice hulls, cellulose, pectin or lignin as the major dietary source of fibre. 3. Food intake was significantly higher in the barley bran group than in the other groups. Both daily weight gain and food conversion of the pectin and alfalfa meal groups were lower than those of the other groups. 4. In the 9-week-old geese, the relative weight and the length of the small intestine did not significantly differ among treatment groups. However, the relative weight was significantly lightest and the caecal length was significantly shortest ( P 0.05) in the pectin group. 5. Activities of amylase and cellulose hydrolases of the caecal contents were also highest in the pectin group. 6. From SEM micrographs, no morphological damage of the villi was observable in the different intestinal segments of the geese in any of the treatments.     

Chemical, physical and sensory changes of small abalone meat during cooking

ABSTRACT:   Small abalone meats were heated at 80°C and 98°C for 0–120 min and the differences in chemical, physical and sensory changes of the cooked meats were investigated. The decrease in moisture and weight and the increase in browning and Hunter's b -value were relatively higher for cooking at 98°C than at 80°C. After cooking for 20–120 min, the total amount of adenosine triphosphate and its related compounds on a dry weight basis decreased by 17–27% at 80°C and by 30–39% at 98°C; the total amount of free amino acids on a dry weight basis changed insignificantly at 80°C but decreased by 22–35% after cooking at 98°C. The meats cooked at 80°C were higher in cutting force whereas the levels in the samples cooked at 98°C did not decrease until samples had been cooked for 60 min. The hydroxyproline content showed little change during cooking except for in samples cooked at 98°C for 120 min, in which the content was found to be low. The extended cooking at 80°C improved the acceptability of small abalone meat, whereas only the acceptability score of aroma increased significantly for cooking at 98°C.     

小麦銹病化学治疗的研究

 治疗剂的应用是防治小麦锈病的新方向,已引起国际上普遍重视,并有不少成功的先例。     

亚马逊黑土及其黑碳颗粒中的真菌丰度和多样性互不相同

Amazonian Dark Earth (ADE) is a highly fertile soil of anthropogenic origin characterized by high levels of charred black carbon (BC).It is considered a model of fertility;however,knowledge on the fungal community structure and diversity inhabiting ADE and its BC particles is scarce.Fungal community structure and diversity of ADE and its BC from four sites under different land uses (three agricultural systems and a secondary pristine forest) in the Brazilian Central Amazon were evaluated by 18S rRNA gene pyrosequencing.Fungal communities in ADE and BC were dissimilar and showed differential abundances of fungal operational taxonomic units (OTUs).Estimated fungal species richness (abundance-based coverage estimate and Chao-1 index) and diversity estimators (Shannon and Simpson's reciprocal indices) were higher in ADE than in BC in all agricultural areas.No differences were observed in those parameters in ADE and BC samples from the secondary forest.Pezizomycotina fungi and OTUs assigned to Cordyceps confragosa,Acremonium vitellinum,Camarops microspora,and Hirsutella rhossiliensis were more abundant in BC particles than in ADE.These findings represent a breakthrough in our understanding of fungal communities in BC particles from ADE,and will be valuable in future studies considering biochar application in soil.     

Bartonella species and their ectoparasites: selective host adaptation or strain selection between the vector and the mammalian host?

A wide range of blood-sucking arthropods have either been confirmed or are suspected as important vectors in Bartonella transmission to mammals, including humans. Overall, it appears that the diversity of Bartonella species DNA identified in ectoparasites is much broader than the species detected in their mammalian hosts, suggesting a mechanism of adaptation of Bartonella species to their host-vector ecosystem. However, these mechanisms leading to the fitness between the vectors and their hosts still need to be investigated.     

控失尿素减施及不同配比对夏玉米产量及氮肥效率的影响

添加纳米矿物类控失剂的控失尿素可通过吸附作用,减少施入土壤后的氮素损失,明确该控失尿素施用量、与常规尿素合理配比在夏玉米上的效应,可为夏玉米一次性施肥、氮肥减施增效提供依据。在不同产量水平(高、中、低产田)土壤上,通过田间试验研究常规尿素(纯氮210 kg hm^–2)、控失尿素常量及减施(减量10%,即纯氮189 kg hm^–2;减量20%,即纯氮168kghm^–2)、控失尿素与常规尿素配施(配比分别为7∶3、5∶5和3∶7)对夏玉米产量、地上部生物量、养分积累量、光合特性及氮肥利用效率的影响。结果表明,氮肥施用可显著提高夏玉米产量,常量控失尿素施用下夏玉米增产率达22.96%～27.55%,在高、中产田上产量较常规尿素有显著提升。与常量控失尿素相比,控失尿素减施10%和20%通过提升穗粒数使得高、中产田夏玉米产量不降低,控失尿素减施20%处理下高产田氮肥利用率高达41.60%。控失尿素与常规尿素7∶3配施下的氮素积累量与常量控失尿素处理无显著差异,高产田和中产田夏玉米产量、秸秆干物重均未显著降低,同时可显著提高低产田夏...     

Genetic diversity of avian paramyxoviruses isolated from wild birds and domestic poultry in Taiwan between 2009 and 2020

Avian paramyxoviruses (APMVs) belonging to the subfamily Avulavirinae within the family Paramyxoviridae. APMVs consist of twenty-two known species and are constantly isolated from a wide variety of avian species around the world. In this study, the APMV isolates obtained from wild birds and domestic poultry during 2009–2020 in Taiwan were genetically characterized by phylogenetic analysis of their complete fusion protein gene or full-length genome. As a result, 57 APMV isolates belonging to seven different species were obtained during this period and subsequently identified as APMV-1 (n=17), APMV-2 (n=1), APMV-4 (n=25), APMV-6 (n=8), APMV-12 (n=2), APMV-21 (n=2) and APMV-22 (n=2). Sanger sequencing was performed to provide 22 full-length genome sequences and 35 complete fusion protein gene sequences for the APMV isolates. Phylogenetic analysis showed that the recovered viruses were closely related to Eurasian strains, except five class I APMV-1 and four APMV-4 isolates were related to North America strains. Our findings provided more evidence for the intercontinental transmission of APMVs between Eurasia and North America by wild birds. In addition, according to the criteria of the classification system based on complete fusion protein gene sequences, three novel genotypes within APMV-2, APMV-12, and APMV-22 were identified. Together, this investigation provided a broader perspective on the genetic diversity, evolution, and distribution of APMVs in multiple avian host species sampled in Taiwan.     

施氮量对超高产夏玉米产量与氮素吸收及土壤硝态氮的影响

【目的】探讨超高产夏玉米（≥12 000 kg•hm-2）节肥增效的适宜氮肥用量。【方法】在夏玉米超高产区连续两年田间试验,研究不同氮肥用量对超高产夏玉米产量、氮代谢、氮素积累、氮肥效率及土壤硝态氮的影响。【结果】超高产区夏玉米施用氮肥两年增产幅度分别为6.76%—9.62%和5.21%—9.80%,夏玉米产量随氮肥用量增加呈先增加后降低趋势,以施氮量300 kg•hm-2产量和收益最佳,经济最佳施氮量为255.40 kg•hm-2;施氮量300 kg•hm-2有利于提高硝酸还原酶和蔗糖磷酸合成酶的活性,促进氮素吸收积累,可维持土壤硝态氮平衡,其氮肥利用率和农学效率两年平均值分别为16.12%和3.69 kg•kg-1。【结论】综合产量、收益、氮素吸收、氮肥利用效率及土壤氮素平衡等方面考虑,豫北地区黏壤质潮土超高产夏玉米合理的氮肥用量为255—300 kg•hm-2。     

IMAGING CHARACTERISTICS OF INTRATHORACIC HISTIOCYTIC SARCOMA IN DOGS

In this retrospective study, two observers independently reviewed thoracic imaging studies of 39 dogs with confirmed histiocytic sarcoma. The most common findings were intrathoracic lymphadenopathy, identified by the first and second observers in 82.1% and 87.2% of dogs, respectively, and pulmonary masses (74.4% and 82.1%). Right middle lung lobe masses were significantly more common than masses in any other lung lobe (P<0.0013), with the majority having a ventral distribution. Sternal and tracheobronchial lymphadenopathy were significantly more common than cranial mediastinal lymphadenopathy (P‐values of 0.0002 and 0.012, respectively). Interobserver agreement regarding distribution of lymphadenopathy and pulmonary masses was good (κ=0.64 and 0.75, respectively). Other findings included pulmonary nodules, pleural effusion, and abnormal pulmonary patterns. In patients with CT examinations, the majority of masses were mildly to moderately enhancing and heterogeneous, poorly marginated, and bronchocentric. Lymphadenopathy and pulmonary masses are the most common intrathoracic findings in dogs with histiocytic sarcoma, and the strong predilection for the ventral aspect of the right middle lung lube may help to differentiate it from other types of neoplasia.