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Soil chemical, biochemical, biological and structural properties were measured in two New Zealand loessial soils that were topsoil-mined 10 and 25 years ago respectively. Measurements at the 10-year site were compared to some earlier measurements made at this site and the data combined in a chronological sequence for analysis. Topsoil mining had a large, detrimental impact on the soil microbial biomass, the earthworm populations, easily mineralizable N and soil enzyme activities. However, most of these properties substantially recovered, to 80-90 per cent of the levels in unmined soils, within 10-25 years of restoration under pasture. In contrast, while total soil C and N were less affected by topsoil mining, their recovery was much slower. Stabilities of macro-aggregates of soil had fully recovered within 10-25 years after topsoil mining. The apparent changes in all the measured properties between 10 and 25 years of restoration were small in comparison with changes between 0-10 years of restoration after topsoil mining. The total C content of both soils under pasture appeared unlikely to attain the levels present in unmined soils. In soils undergoing restoration, the ratio of microbial C/total soil C may be a useful index of soil ‘biological stability’. Sulphatase activity may reflect the recovery of pasture production. 相似文献
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Postlarvae or juveniles of Macrobrachium rosenbergii produced in Mauritius are sold at unit price to prawn farmers and are counted individually by hand. This process is time consuming, labour intensive, subject to error and may stress the juveniles; safe rapid electronic counting would be highly advantageous.The design of the hydraulic component of a successful counter prototype, Type A, is described. The counter has 10 parallel channels through which juveniles are carried in a water current. The detection of juveniles passing through these channels can be done photo-electrically. The counter accepts juveniles up to 15 mm in length (eye orbit to tip telson) and has the significant advantage that it can be constructed using only simple hand tools.Survival and growth rate tests, and microscopic examination for damage to appendages of juveniles were done on machine counted and hand counted juveniles. There was no reduction of growth or survival caused by passage through the counter by comparison with the hand counting method, and it is concluded that the counter is not detrimental to juvenile prawns.About 70% of all juveniles tested showed highly variable amounts of damage. The great majority of this damage appears to originate in the hatchery tanks, prior to the experimental treatments. Only in the case of antennules could the use of the counter be shown to increase damage significantly, i.e. from 25% to 38%, but this has no detectable effect on subsequent survival or growth.This appears to be the first counter made specifically for counting macroscopic crustacean juveniles, and via suitable scaling, adaptation to counting other species of crustaceans, fish larvae, etc., should be possible. 相似文献
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Gillooly JF Brown JH West GB Savage VM Charnov EL 《Science (New York, N.Y.)》2001,293(5538):2248-2251
We derive a general model, based on principles of biochemical kinetics and allometry, that characterizes the effects of temperature and body mass on metabolic rate. The model fits metabolic rates of microbes, ectotherms, endotherms (including those in hibernation), and plants in temperatures ranging from 0 degrees to 40 degrees C. Mass- and temperature-compensated resting metabolic rates of all organisms are similar: The lowest (for unicellular organisms and plants) is separated from the highest (for endothermic vertebrates) by a factor of about 20. Temperature and body size are primary determinants of biological time and ecological roles. 相似文献
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Soon FF Ng LM Zhou XE West GM Kovach A Tan MH Suino-Powell KM He Y Xu Y Chalmers MJ Brunzelle JS Zhang H Yang H Jiang H Li J Yong EL Cutler S Zhu JK Griffin PR Melcher K Xu HE 《Science (New York, N.Y.)》2012,335(6064):85-88
Abscisic acid (ABA) is an essential hormone for plants to survive environmental stresses. At the center of the ABA signaling network is a subfamily of type 2C protein phosphatases (PP2Cs), which form exclusive interactions with ABA receptors and subfamily 2 Snfl-related kinase (SnRK2s). Here, we report a SnRK2-PP2C complex structure, which reveals marked similarity in PP2C recognition by SnRK2 and ABA receptors. In the complex, the kinase activation loop docks into the active site of PP2C, while the conserved ABA-sensing tryptophan of PP2C inserts into the kinase catalytic cleft, thus mimicking receptor-PP2C interactions. These structural results provide a simple mechanism that directly couples ABA binding to SnRK2 kinase activation and highlight a new paradigm of kinase-phosphatase regulation through mutual packing of their catalytic sites. 相似文献
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Waghorn TS Leathwick DM Rhodes AP Lawrence KE Jackson R Pomroy WE West DM Moffat JR 《New Zealand veterinary journal》2006,54(6):271-277
AIM: To establish the prevalence of anthelmintic resistance in parasitic nematodes on sheep farms in New Zealand. METHODS: A cross-sectional prevalence study was conducted, using a standardised faecal nematode egg count (FEC) reduction (FECR) test (FECRT) for ivermectin, at a full (0.2 mg/kg) and half (0.1 mg/kg) dose rate, and albendazole, levamisole and albendazole-levamisole in combination, on 60 lambs (n=10 per group) on farms selected from throughout New Zealand. Farms that conformed with selection criteria were chosen at random (n=80) or with a history of suspected resistance to macrocyclic lactone (ML) anthelmintics (n=32). Resistance to an anthelmintic was inferred when there was <95% reduction in FEC 7-10 days after treatment. Larval cultures were performed for all control groups and for treated groups for which resistance was evident. RESULTS: Of the farms randomly selected, 36% showed > or =95% FECR for all anthelmintics tested; resistance to ivermectin at 0.1 and 0.2 mg/kg liveweight was evident on 36% and 25% of these farms, respectively. Resistance to both ivermectin (0.2 mg/kg) and levamisole was evident on 8/80 (10%) farms, to ivermectin and albendazole on 10/80 (13%) farms, and to ivermectin, levamisole and albendazole on 6/80 (8%) farms. The prevalence of resistance to a half dose of ivermectin tended to be more prevalent on farms with a history of suspected ML resistance (p=0.06). Resistance to albendazole was seen across all the main parasite genera, and to levamisole in Nematodirus, Ostertagia (= Teladorsagia) and Trichostrongylus species. Resistance to ivermectin was dominated by Ostertagia spp, although Cooperia, Nematodirus and Trichostrongylus species were also implicated. CONCLUSION: Anthelmintic resistance in parasitic nematodes of sheep is common in New Zealand. Not only was resistance to albendazole and levamisole common, but resistance to the ML, ivermectin, was at a higher prevalence than expected. Sheep farmers and advisors in New Zealand need to re-evaluate the way they manage parasites, and more research is urgently needed if the steady decline in anthelmintic susceptibility is to be halted. 相似文献
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R Jackson AP Rhodes WE Pomroy DM Leathwick DM West TS Waghorn 《New Zealand veterinary journal》2013,61(6):289-296
AIM: To provide information on current farmers’ opinions and farming practices thought to be related to anthelmintic resistance, and to test for associations between the presence of anthelmintic resistance and management practices on beef cattle- rearing farms in the North Island of New Zealand. METHODS: A study using an interview-based questionnaire about management of internal parasites was conducted on 62 beef cattle-rearing farms in the North Island of New Zealand, using case-control analyses to test for associations between management practices and the presence or absence of resistance to ivermectin or albendazole. Resistance was inferred from faecal nematode egg count (FEC) reduction (FECR) tests (FECRTs) when there was <90% reduction in FEC 7-10 days after treatment of calves <12 months of age. RESULTS: Of the 59 farmers who completed the questionnaire, most (n=40) ranked parasites highly, and at about the same level as quality and quantity of feed, as important production-limiting factors for their enterprises. In contrast, anthelmintic resistance was not perceived to be a problem on 13 farms, and its importance was rated low on 24, moderate on 15, and high on only six farms. Despite all farms having planned parasite control programmes, there was heavy reliance on clinical signs of parasitism to determine frequency of treatments. About one in three farmers with beef breeder cows routinely treated their calves at marking, one in five treated mixed-age cows, and almost half treated rising 2-year-old cows before calving. One in four farmers used anthelmintics on calves on 8–12 occasions in their first year of life. Co-grazing with other species was rare, but follow-on grazing within 3 months after older cattle or sheep was common. On most farms, grazing cattle was restricted to part of the farm, a finding with implications for parasite control and persistence of larvae in refugia. Macrocyclic lactone (ML) anthelmintics or their combinations with other action families were currently, and for the past 5 years, used more frequently than benzimidazoles and levamisole, and benzimidazole-levami- sole combinations. The prevalence of resistance to ivermectin was high (82%) and no plausible model of associations could be constructed from the data. The prevalence of resistance to albendazole was 60%, and the risk of resistance increased as the number of rising 1-year-old cattle present mid-winter increased, and decreased as the number of breeding cows >2 years old present mid-winter increased. CONCLUSION: It is clear that in practice anthelmintic resistance is a secondary consideration to obtaining productivity advantages from the use of anthelmintics in beef cattle. Farmers’ opinions were divided on many issues and the overall impres- sion was of confused and diverse thinking regarding the principles of the use of anthelmintics. The overall outlook regarding anthelmintic resistance in cattle is bleak unless the need for integrated and long-term research activities is acted upon soon. 相似文献
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West D 《New Zealand veterinary journal》1983,31(9):152-156
A study was made of various aspects of 53 naturally occurring cases of foot abscess in sheep in New Zealand. Radiographic techniques were used to follow the progress of the lesion and from this it was concluded that foot abscess could be defined as an infection of the distal interphalangeal joint. The term foot abscess was considered preferable to the term "infective bulbar necrosis". The attack rate of foot abscess was reported to be higher for rams than for ewes and the condition was less prevalent during summer. Once infection was established in the distal interphalangeal joint a relatively uniform and thus predictable series of events took place that inevitably resulted in some permanent damage and deformity to the digit. If rupture of the axial collateral ligaments occurred, the joint became unstable and the degree of permanent deformity was greater. Treatment with antibiotics did not appear to markedly affect the progress of the lesion or the outcome, once infection had become established in the joint. Although the prognosis for complete recovery was poor, in most cases the foot healed sufficiently after a period of about two months to allow the animal to walk normally. 相似文献