Phosphofructokinase and Malate Dehydrogenase Participate in the In Vitro Maturation of Porcine Oocytes

Oocyte maturation depends on the metabolic activity of cumulus–oocyte complex (COC) that performs nutritive and regulatory functions during this process. In this work, the enzymes [phosphofructokinase (PFK) and malate dehydrogenase (MDH)] were tested to elucidate the metabolic profile of porcine COCs during the in vitro maturation (IVM). Enzymatic activity was expressed in U/COC and U/mg protein (specific activity) as mean ± SEM. In vitro maturation was performed with 2‐oxoglutarate (5, 10 and 20 mm ) or hydroxymalonate (30, 60 and 100 mm ) inhibitors of PFK and MDH, respectively. The PFK and MDH activities (U) remained constant during maturation. For PFK, the U were (2.48 ± 0.23) 10^?5 and (2.54 ± 0.32) 10^?5, and for MDH, the U were (4.72 ± 0.42) 10^?5 and (4.38 ± 0.25) 10^?5 for immature and in vitro matured COCs, respectively. The specific activities were significantly lower after IVM, for PFK (4.29 ± 0.48) 10^?3 and (0.94 ± 0.12) 10^?3, and for MDH (9.08 ± 0.93) 10^?3 and (1.89 ± 0.10) 10^?3 for immature and in vitro matured COCs, respectively. In vitro maturation percentages and enzymatic activity diminished with 20 mm 2‐oxoglutarate or 60 mm hydroxymalonate (p < 0.05). Viability was not affected by any concentration of the inhibitors evaluated. The U remained unchanged during IVM; however, the increase in the total protein content per COC provoked a decrease in the specific activity of both enzymes. Phosphofructokinase and MDH necessary for oocyte IVM would be already present in the immature oocyte. The presence of inhibitors of these enzymes impairs the meiotic maturation. Therefore, the participation of these enzymes in the energy metabolism of the porcine oocyte during IVM is confirmed in this study.     

英国Culm河河漫滩沉积物中磷素时空变化研究

河漫滩上沉积物的沉积伴随着与沉积物相结合的营养和污染物质的沉积。已有研究表明,河漫滩是与沉积物相结合的磷的沉积地。采用137Cs技术,并结合河漫滩沉积物中全磷(TP)含量的剖面信息,可以调查近年河漫滩沉积物中的TP含量,计算TP储量。本文根据英国Devon郡Culm河8个河漫滩采样环沉积物中的数据,重建了在过去40～50年内TP储量和含量的变化。结果表明,在Culm河河漫滩沉积物中,在1963～2000年间TP平均含量为0.60～1.96gkg-1,该值自河流上游向下游和自过去到现在有逐渐增加的趋势;在相同的河漫滩沉积物中,在相同的时期内TP储量为18.62～435.48gm-2,即0.49～11.46gm-2a-1,最大值出现在河流的中游。     

A preliminary assessment of the potential for using Pbex measurement to estimate soil redistribution rates on cultivated slopes in the Sichuan Hilly Basin of China

In order to assess its potential for estimating soil redistribution rates, the naturally occurring fallout radionuclide ²¹⁰Pb_ex has been used in parallel with ¹³⁷Cs, derived from the atmospheric testing of nuclear weapon testing in the 1950s to 1970s, to estimate rates of soil redistribution on a sloping field with traditional erosion control measures located near Jiajia Village, Jianyang County, in the Sichuan Hilly Basin of China. The local ²¹⁰Pb_ex reference inventory of 12,860 Bq m^− 2 is higher than those reported for many other areas of the world and may reflect the influence of cloudy weather in preventing ²¹⁰Pb released to the atmosphere across the local region moving up into the upper troposphere, where is would be more widely dispersed. The mean ²¹⁰Pb_ex and ¹³⁷Cs inventories measured in cores collected from the upper part of the field with an average slope of 10° were 8028 Bq m^− 2 and 993 Bq m^− 2, respectively, and the equivalent values for the lower part of the field, where the slopes are steeper (20°) were 11,388 Bq m^− 2 and 1299 Bq m^− 2. The pattern of post-fallout ²¹⁰Pb_ex and ¹³⁷Cs redistribution on the sloping field reflects not only the effects of water erosion and redistribution by tillage, but also the local traditional practice of “Tiaoshamiantu”, whereby sediment trapped in the ditches is returned to the fields by the farmer. The estimates of annual rates of soil loss provided by the ²¹⁰Pb_ex measurement are closely comparable with those derived from the ¹³⁷Cs measurements and are consistent with existing knowledge for the study area. The results obtained from this study confirm the potential for using ²¹⁰Pb_ex measurement to estimate soil erosion rates over medium-term timescale of 50–100 years. By combining the estimates of erosion rates provided by the ²¹⁰Pb_ex and ¹³⁷Cs measurements, the weighted mean net soil loss was estimated to be 48.7 t ha^− 1 year^− 1 from the upper subfield and 16.9 t ha^− 1 year^− 1 from the lower subfield. These rates are considerably lower than the erosion rates obtained from runoff plot measurements in the local area. It is suggested that the traditional erosion control practices and the practice of “Tiaoshamiantu” have a significant effect in reducing soil loss and conserving valuable cultivated soil on sloping fields in the Sichuan Hilly Basin.     

Investigating Spatial Patterns of Overbank Sedimentation on River Floodplains

Attempts to study spatial patterns of overbank sedimentation on river floodplains commonly face important operational and sampling problems in documenting deposition rates. Recent advances in the application of fallout radionuclides (¹³⁷Cs and unsupported²¹⁰Pb) to the estimation of medium-term rates of overbank sedimentation offer an essentially unique opportunity to assemble detailed distributed data sets for medium term deposition rates. Such data can afford a valuable basis for investigating the complex relationship between sedimentation rates and floodplain microtopography and morphology and flow hydraulics. This paper reports the results of an investigation of the spatial pattern of overbank sedimentation rates on the floodplain of the River Culm, Devon, UK. Caesium-137 and unsupported²¹⁰Pb measurements have been used to establish the general pattern of deposition rates along an 11 kilt reach and more intensive measurements have been employed to document the detailed pattern existing within a small area subject to frequent inundation. The resultant data have been used as a basis for interpreting the major controls on the observed patterns.     

Fine-earth translocation by tillage in stony soils in the Guadalentin, south-east Spain: an investigation using caesium-134

Tillage erosion is increasingly recognised as an important soil erosion process on agricultural land. In view of its potential significance, there is a clear need to broaden the experimental database for the magnitude of tillage erosion to include a range of tillage implements and agricultural environments. The study discussed in this paper sought to address the need for such data by examining tillage erosion by a duckfoot chisel plough in stony soils on steep slopes in a semi-arid environment. Results of the investigation of coarse fraction (rock fragment) translocation by tillage in this environment have been presented elsewhere and the paper focuses on tillage translocation and erosion of the fine earth. Tillage translocation was measured at 10 sites, representing both upslope and downslope tillage by a duckfoot chisel plough on five different slopes, with tangents ranging from 0.02 to 0.41. A fine-earth tracer, comprising fine earth labelled with ¹³⁴Cs, was introduced into the plough layer before tillage. After a single pass of the plough, incremental samples of plough soil were excavated and sieved to separate the fine earth from the rock fragments. Translocation of the fine-earth tracer was established by analysing the ¹³⁴Cs content of the samples of fine earth. These data were used to establish translocation distances for each combination of slope and tillage direction. Translocation distances of the fine earth were not significantly different from translocation distances of the coarse fraction. For all sites, except uphill on the 0.41 slope, translocation distances were found to be linearly related to slope tangent. The soil flux due to tillage for each site was calculated using the translocation distance and the mass per unit area of the plough layer. For slopes with tangents <0.25, the relationship between soil flux and tangent was linear and the soil flux coefficient derived was 520–660 kg m⁻¹ per pass. This is much larger than the coefficients found in other studies and this high magnitude is attributed to the non-cohesive nature and high rock fragment content of the soil in this investigation. A second contrast with previous studies was found in non-linearity in the relationship between soil flux and tangent when steeper slopes were included. This was a product of variation in plough depth between the steepest slopes and the remainder of the study area. On the basis of the study it is suggested that an improved understanding of tillage erosion may be obtained by considering the dual processes of tillage detachment (mass per unit area of soil subject to tillage) and tillage displacement (equivalent to translocation distance per pass) in assessing, comparing and modelling tillage translocation. An improved model is proposed that recognises the complexity of soil redistribution by tillage, provides a framework for process-based investigation of the controls on tillage fluxes, and allows identification of potential self-limiting conditions for tillage erosion.     

Soil erosion and redistribution on cultivated and uncultivated land near las bardenas in the central Ebro river Basin,Spain

The semiarid regions of Spain, including the central part of the Ebro River basin, are under threat due to desertification. Severe erosion, as a result of poor land management, has led to degradation of the soil resource, and there is a clear need for quantitative erosion rate data to evaluate the problem. This study aimed to examine the potential for using caesium-137 to identify the patterns and rates of soil erosion and redistribution within this semiarid environment. Samples for the determination of caesium-137 were collected from uncultivated slopes and cultivated valley floor sites near the head and outlet of a small representative basin in the Las Bardenas area. The measured patterns of caesium-137 mobilization, redistribution and export provide a semiquantitative indication of the variation in erosion within the study site. Calibration of the caesium-137 measurements, taking account of the differing behaviour of radiocaesium on cultivated and uncultivated land, allows estimation of the actual rates of erosion and deposition involved. The results show (1) the erosion rates on the cultivated land (1.6–2.5 kg m⁻² yr⁻¹) are typically more than five times those seen on the uncultivated land (0.2–0.4 kg m⁻² yr⁻¹), and (2) erosion on the uncultivated land is significantly less severe at the head of the basin than at the outlet. Study of the vegetation cover suggests that lower growing shrubs and grasses may be more effective in reducing erosion in this environment than trees.     

Infectivity and virulence of Australian strains of Moraxella bovis for the murine and bovine eye in relation to pilus serogroup sub-unit size and degree of piliation

The degree of piliation of 29 haemolytic and 4 non-haemolytic Australian strains of Moraxella bovis representing 7 different pilus antigen groups was determined. The infectivity and virulence for the eye was measured in steroid-treated mice and in cattle. Non-piliated strains failed to infect the murine eye. Most moderately or heavily piliated strains reproducibly produced the highest infectivity and virulence scores in mice when compared with lightly or very lightly piliated strains (p less than 0.05). Non-haemolytic, piliated strains were infective and in one instance virulent for mice. Almost similar levels of infectivity and virulence were observed for 7 representative haemolytic strains tested in both cattle and mice. The relative molecular weight of pilin sub-units was compared using sodium dodecyl-sulphate polyacrylamide gel electrophoresis. Three classes of pili, alpha, beta and gamma of ascending sub-unit size were identified among the 7 pilus antigen serogroups. Pilin sub-unit size bore no relationship to the degree of piliation but most strains that were highly virulent in mice and cattle expressed alpha and gamma sub-units. Some strains appeared capable of switching from alpha to beta or form beta to gamma sub-unit production.     

人工授精使肉猪利润最大化

商业性养猪生产是通过人工授精(AI)来实现的,能对其产生影响的技术即使有,也是极少数.口本养猪生产人员于1947年首次倡导应对猪实行人工授精,然而直到20世纪80年代才得以经常性地应用,从而使遗传学的常规移动可跨越更大的距离.     

Integrated assessment of catchment suspended sediment budgets: a Zambian example

In many developing countries, the management of sediment‐related environmental problems is severely hampered by a lack of information on sediment mobilization and delivery in river basins. The sediment budget concept represents a valuable framework for assembling such information, which can, in turn, be used to assist with the design and implementation of soil erosion and sediment control policies. However, the information necessary to construct a catchment sediment budget is difficult to assemble. Against this background, an integrated approach to establishing a catchment suspended sediment budget, involving a river monitoring station, the use of ¹³⁷Cs measurements to estimate soil erosion and deposition and floodplain accumulation rates within the catchment, and sediment source fingerprinting, has been developed and tested in the 63 km² catchment of the upper Kaleya River in southern Zambia. The approach developed not only provides detailed information on individual components of the suspended sediment delivery system, but also permits the establishment of the overall catchment sediment budget. A sediment budget for the upper Kaleya catchment is presented and both its key features and its wider implications for catchment management are discussed. Copyright © 2001 John Wiley & Sons, Ltd.