支链氨基酸对哺乳动物蛋白质周转的影响及机理研究进展

支链氨基酸(branch chain amino acids,BCAA)作为一种重要的信号调节分子,参与动物机体蛋白质周转等重要生物学过程。此过程涉及两条进化上高度保守的感知氨基酸水平的信号通路,对氨基酸丰度敏感的哺乳动物雷帕霉素靶蛋白(mammals target of rapamycin,mTOR)和感知一种或多种未负载氨基酸的t RNA而活化的一般氨基酸调控阻遏蛋白2(general amino acid control non-derepressible 2,GCN2)。本文主要探讨BCAA对mTOR和GCN2通路的影响,旨在阐述BCAA调控蛋白质合成和降解的机制,为研究调控哺乳动物蛋白质周转的机理提供借鉴,为BCAA在饲料添加剂应用方面提供理论依据。     

高效奶牛饲养技术

高效奶牛饲养技术西北农业大学姚军虎．金公亮第五讲奶牛饲养管理技术要点（上）一、犊牛的培育（出生——断奶）哺乳期，犊牛的生理机能处于急剧变化阶段，可塑性大，科学的饲养管理措施，可使犊牛充分显现双亲的优良遗传特性，纠正或改良某些遗传缺陷，促进牛群质量的不...     

D-LysAsnProTyr四肽对肉仔鸡免疫及主要生产性能的影响

将体重、发育及健康状况接近的300羽7日龄肉仔鸡随机等分为5个处理，每个处理设3重复，每重复20羽。试鸡用D-LysAsnProTyr四肽＋3头份新城疫Ⅳ系疫苗生理盐水混合液滴鼻，剂量为50μl／只，D-LysAsnProTyr四肽水平为：0μg／只（对照组），15μg／只，20μg／只，25μg／只，30μg／只。测定新城疫抗体水平及淋巴细胞ANAE阳性百分率，计算全期平均日增重、采食量和料肉比。结果表明，D-LysAsnProTyr四肽在20～25μg／只鸡剂量对ND抗体有明显的提高作用，不改变抗体变化规律；显著提高肉仔鸡血液中淋巴细胞阳性百分率，10～42日龄20μg／只和25μg／只试验组分别比对照组提高2．03～9．47个百分点和2．36～9．22个百分点，淋巴细胞阳性百分率的增减变化规律与对照组相一致，说明适宜剂量D-LysAsnProTyr四肽可以显著提高肉仔鸡对ND的体液免疫和细胞免疫。20μg／只、25μg／只组试期末体重、平均日增重显著高于15μg／只、30μg／只组（P〈0．05）。与20μg／只、25μg／只组相比，对照组日增重、饲料转化率有降低趋势（P〉0．05）。各处理组对肉仔鸡日均采食量无显著影响（P〉0．05）。     

优质豆饼替代品—科威418

科威418是西北农业大学有关科研人员经多年研究而制成的高科技新产品,内含尿素、营养保护剂、酶及18种微量元素,粗蛋白质当量为100～105％。该产品是牛羊理想     

功能性低聚糖及其在断奶仔猪饲养中的应用

低聚糖又称寡聚糖、寡糖,是由2～10个单糖经脱水缩合由糖苷键连接形成的具有直链或支链的低度聚合糖类。分子通式一般表示为(C6H10O5)n,n=2～10,相对分子质量约为300～2000u。按生物学功能可分为普通低聚糖和功能性低聚糖两大类[1]。蔗糖、麦芽糖、海藻糖等主要的糖苷键为α-1,4型,它们能被机体消化吸收,产生能量,称为普通低聚糖。功能性低聚糖含有α-1,6、β-1,2型糖苷键,如异麦芽低聚糖(α-GOS)、果寡糖(FOS)、甘露寡糖(MOS)等,不能被单胃动物直接利用,但可以促进双歧杆菌和乳酸杆菌等有益菌的增殖,动物营养学界称之为“Probiotic”…     

Unsaturated vitamin b(12) binding capacity in human and ruminant blood serum - a comparison of techniques including a new technique by high performance liquid chromatography

A new technique by High Performance Liquid Chromatography (HPLC-gel permeation) shows promise as a tool to separate and quantitate the Unsaturated Vitamin B(12) Binding Capacity (UBSC) of the individual Vitamin B(12) binders in blood serum. This method, although not as rapid as protein-coated charcoal or cellulose separation techniques, is more applicable for use with large numbers of samples than gel filtration. The use of a radioactivity detector to monitor the eluant from the column permitted automation of the method. Comparable results for UBBC and for the UBBC of individual binders were obtained when samples were analyzed by gel filtration and HPLC. The HPLC method proved suitably precise and the recovery of added cyanocobalamin was acceptable. It is proposed that HPLC be the method of choice for measurement of the USBC of binders of Vitamin B(12) in blood serum.     

Serotypes of Yersinia pseudotuberculosis recovered from domestic livestock

The serological identity of 234 strains of Yersinia pseudotuberculosis recovered from domestic animals and birds in New Zealand was determined by slide agglutination test. Thirty strains were also examined by tube agglutination test. The strains were isolated from cattle (56), sheep (8), deer (117), goats (13), pigs (7), rabbits (6), guinea pigs (5), and aviary species of birds (22). All strains were isolated from animals or birds which had died or shown signs of ill health and amongst which diarrhoea was a common feature. Serotype I accounted for 23% (53) of strains, serotype II for 13% (30) of strains and serotype III for 64% (151) of strains. It was concluded that further investigations on the prevalence and serological identity of strains recovered from clinically healthy animals mav provide useful information in assessing the significance of various serotypes as a cause of disease in livestock.