Bradyarrhythmia in an anaesthetised, elderly, hypertensive cat

A 14-year-old neutered male domestic shorthaired cat was presented to the University Veterinary Centre Sydney for evaluation and treatment of dental disease. This cat developed an unusual bradyarrhythmia under anaesthesia. The possible causes and treatment of the dysrythmia are discussed.     

Roots in irrigated clay soils: Measurement techniques and responses to rootzone conditions

Summary This paper reviews research carried out at the Griffith Laboratory in Australia over the last decade on techniques for, and results of, observations of roots in irrigated clay soils. Our results emphasise the adaptability of root systems to rootzone conditions. Experiences with techniques for observing roots non-destructively in the field and both non-destructively and destructively in lysimeters are described. We concluded that the minirhizotron technique, applied in the field, was unreliable under our conditions. Horizontal root observation tubes were used in lysimeters to measure root length density (RLD) and to assess whether roots were clumped together or randomly distributed. Destructive sampling and measurement of RLD was used to establish a theoretical relationship between root intercept counts along the tubes and RLD. The application of image analysis to both destructive and non-destructive sampling in the lysimeters is outlined. The non-destructive lysimeter studies showed that roots were significantly clumped. Analysis of root intercept and root hole counts on the faces of sample cubes taken from the lysimeters showed root distribution was anisotropic over the whole soil profile for both safflower and wheat. There were many more roots and root holes present in the sampled soil cubes than was indicated by independent sampling for washed out RLD. Safflower appeared to have a faster turnover of roots than did wheat or maize. Lysimeters, equipped with horizontal root observation tubes, enabled studies to be made of many factors affecting root growth. Soils affect where and how fast roots grow, although there is also a strong species interaction. For example, soybean roots proliferated above a fresh water table in one soil but not in another; wheat had little tendency to proliferate above the water table in either soil. In wet soils, roots cease to grow once soil oxygen levels decrease below 10 mg O₂ l _soil ^-1 . This level should form the basis for soil drainage criteria. In drying soils, roots will grow successively into soil regions containing soil water: the level of adaptation being determined by soil conditions, crop growth stage and level of evaporative demand. The methods of root observation used in our studies have given quantitative assessment of root distribution. However, further research is needed to link horizontal and vertical root distribution and root adaptation more strongly to crop development and soil conditions.     

Effects of soil type on soybean crop water use in weighing lysimeters

Summary Different soils are known to affect the amount and distribution of both available water and roots. Optimising irrigation water use, especially when shallow water-tables are present requires accurate knowledge of the root zone dynamics. This study was conducted to determine the effect of two soil types on root growth, soil water extraction patterns, and contributions of a water-table to crop evaporation (E). Two weighing lysimeters (L1 and L2) with undisturbed blocks of soil were used. The soil in L1 had higher hydraulic conductivity and lower bulk density than that in L2. Well watered conditions were maintained by irrigation for the first 110 days from sowing (DFS). Root length density (RLD) was calculated from observations made in clear acrylic tubes installed into the sides of the lysimeters. Volumetric soil water contents were measured with a neutron probe. A water-table (EC = 0.01 S m^-1) was established 1 m below the soil surface 18 DFS. RLD values were greater in L1 than L2 at any depth. In L1, maximum RLD values (3 × 10⁴ m m^-3) were measured immediately above the water-table at physiological maturity (133 DFS). In L2, maximum RLD values (1.5 × 10⁴ m m^-3) were measured at 0.42 m on 120 DFS and few roots were present above the water-table. From 71 to 74 DFS, 55 and 64% of E was extracted from above 0.2 m for L1 and L2, respectively. In L2, extraction was essentially limited to the upper 0.4 m, while L1 extraction was to 0.8 m depth. Around 100 DFS the water-table contributed 29% (L1) and 7% (L2) of the water evaporated. This proportion increased rapidly as the upper soil layers dried following the last substantial irrigation 106 DFS. Over the whole season the water-table contributed 24% in L1 and 6.5% in L2 of total E.     

Dominant sources of dietary carbon and nitrogen for shrimp reared in extensive rice-shrimp ponds

Stable isotope analysis was used to determine the sources of dietary nitrogen (N) and carbon (C) for shrimp during the rearing phase in extensive rice‐shrimp ponds in My Xuyen and Gia Rai districts, Vietnam. Farm‐made feed was added as a food source in shrimp ponds in My Xuyen district, and based on stable isotope analyses, was generally a poor dietary source. The commercial formulated feed used in Gia Rai also appeared to contribute little directly to the nutritional needs of the shrimp. In contrast, the natural biota in all ponds appeared to contribute substantially. In particular, biota from beam trawls and benthic organic matter were the most likely sources of nutrition in My Xuyen ponds, while benthic organic matter was the main source in Gia Rai ponds. δ¹⁵N ratios in the natural biota in My Xuyen farms decreased over the growing season, suggesting increased N fixation in the case of the benthic organic matter reaching values as low as 1‰. This suggests N‐limitation in the ponds and that natural biota become increasingly dependent on N fixed by algae and/or other microorganisms. There is the potential to promote the growth of the plankton and hence, the other natural biota, by the judicious addition of fertilizer.     

Feline pyothorax - new insights into an old problem: part 2. Treatment recommendations and prophylaxis

Until recently, pyothorax in the cat has been generally considered to have a poor prognosis. However, it has become clear that most cats that survive the first 48 h following presentation can be successfully treated with aggressive medical management. In this second part of a two-part review, logical guidelines for the management of the disease are discussed, with particular emphasis on antimicrobial selection. Patient stabilisation and supportive care, techniques for pleural space drainage and lavage and indications for surgery are reviewed.     

Lignin als biologische Barriere gegen Schimmelpilze in Innenräumen

Many different fungi grow indoors as mold in the presence of moisture. Some of these fungi can cause allergic or toxic reactions, while a few may cause infections in susceptible individuals. A comprehensive treatment of this complex topic would take volumes. Therefore many groups search for alternative management strategies of these fungi. It has been tested if the growth of nine fungi (Alternaria alternata, Botrytis cinerea, Cladosporium herbarum, Fusarium culmorum, F. solani, F. verticillioides, Mucor sp., Penicillium sp. and Trichoderma sp.) which are common in interior buildings could be inhibited by mixing lignin into wall plaster. In maltextract agar lignin concentrations above 10 % inhibited mould growth except for Penicillium sp. However, none of the mould species showed and degradation of lignin. Further investigations on a lignin-plaster-agar, which contained only plaster as nutrient and had a high pH at 8, showed no inhibition on the tested fungi (Alternaria alternata, Fusarium verticillioides, Mucor sp., Trichoderma sp. and Penicillium sp.) at concentrations up to 5 %. Higher lignin concentrations lead to insufficient quality of building materials, e. g. cracking. Furthermore the color of the building materials turned from greyish-white in an exotherm reaction into brown, become not any more suitable for construction purposed.     

Detection of QTL for forage yield,lodging resistance and spring vigor traits in alfalfa (Medicago sativa L.)

Alfalfa (Medicago sativa L.) is an internationally significant forage crop. Forage yield, lodging resistance and spring vigor are important agronomic traits conditioned by quantitative genetic and environmental effects. The objective of this study was to identify quantitative trait loci (QTL) and molecular markers associated with increased forage yield, resistance to lodging, and spring vigor. A backcross population composed of 128 progeny was developed by crossing the breeding parents DW000577 (lodging susceptible) and NL002724 (lodging-resistant) and back-crossing an individual F₁ plant to the maternal parent (i.e. DW000577). A linkage map of NL002724 was developed based upon the segregation of 236 AFLP, SRAP, and SSR markers among the backcross progeny. The markers were distributed among 14 linkage groups, covering an estimated recombination distance of 1497.6 centiMorgans (cM). Replicated clones of both parents and backcross progeny were evaluated in the field for estimated forage yield, lodging, and spring vigor in Washington and Wisconsin during 2007 and 2008. Significant QTL were found for all three traits. In particular, two QTL for lodging resistance were identified that explained ≥14 % of trait variation, and were significant in all years and locations. Major QTL explaining over 25 % of trait variation for forage yield were detected in multiple environments at two separate locations on chromosome III. Several QTL for spring vigor were located in the same or similar positions as QTL for forage yield, possibly explaining the significant correlation between these traits. Molecular markers associated with the aforementioned QTL were also identified.     

Lithothamnion muelleri Controls Inflammatory Responses,Target Organ Injury and Lethality Associated with Graft-versus-Host Disease in Mice

Lithothamnion muelleri (Hapalidiaceae) is a marine red alga, which is a member of a group of algae with anti-inflammatory, antitumor, and immunomodulatory properties. The present study evaluated the effects of treatment with Lithothamnion muelleri extract (LM) in a model of acute graft-versus-host disease (GVHD), using a model of adoptive splenocyte transfer from C57BL/6 donors into B6D2F1 recipient mice. Mice treated with LM showed reduced clinical signs of disease and mortality when compared with untreated mice. LM-treated mice had reduced tissue injury, less bacterial translocation, and decreased levels of proinflammatory cytokines and chemokines (interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), chemokine (C-C motif) ligand 2 (CCL2), chemokine (C-C motif) ligand 3 (CCL3) and chemokine (C-C motif) ligand 5 (CCL5)). The polysaccharide-rich fraction derived from LM could inhibit leukocyte rolling and adhesion in intestinal venules, as assessed by intravital microscopy. LM treatment did not impair the beneficial effects of graft-versus-leukaemia (GVL). Altogether, our studies suggest that treatment with Lithothamnion muelleri has a potential therapeutic application in GVHD treatment.