绵羊肺炎支原体恒温热隔绝式PCR方法的建立

为建立绵羊肺炎支原体(Mo)感染疾病的现场便捷化诊断方法,本研究选择Mo高度保守区域tuf基因设计合成一对特异性引物和探针,建立了恒温热隔绝式PCR(iiPCR)方法并对其特异性、灵敏性进行评价。结果显示,建立的iiPCR方法仅对Mo的典型菌株和其临床分离菌株的DNA呈阳性结果,具有较强的特异性;对Mo基因组DNA的检出下限为24fg/μL,具有较高的敏感性。利用该方法检测临床样品,结果显示该方法可以从临床采集的羊鼻拭子及支气管拭子中检测出MoDNA,对扩增产物的测序表明检测结果具有较高的准确性。本研究建立的iiPCR方法为Mo的检测及其感染疾病的诊断提供了快速、便捷的技术手段。     

液压控制履带自走式温室三七收获机设计与试验

针对温室三七收获机械化程度低、收获效率低、破损率高等问题,设计一种液压控制履带自走式温室三七收获机。在满足农艺要求的基础上,使用履带式行走底盘,并对其进行运动学分析,利用RecurDyn对整机直线行走特性进行仿真分析,单侧牵引动力为1357N,质心波动平稳;建立了根土混合物运动学模型,并确定挖掘部分最优结构参数。利用FluidSIM软件和GX Developer软件开发平台分别设计了本地操作系统和远程控制系统,使整机具有行走、传动和升降功能。田间试验表明：在不同控制模式下,整机能顺利完成直行、转弯和挖掘装置升降等功能,液压控制系统和机械部分工作顺畅,直线行走稳定,行走偏移量为0.49%,液压缸前进、后退速度均为0.22m/s,误差满足要求;平均伤根率为1.77%,平均损失率为1.48%,无明显埋根现象,满足三七收获机性能要求。     

D奇宝优527在长汀作烟后稻表现及高产栽培技术

D奇宝优527系福建省尤溪县良种生化研究所与福建省种子总站合作用天奇宝A与蜀恢527杂交选育而成的晚籼三系杂交水稻组合。2004年通过福建省农作物品种审定委员会审定（闽审稻2004008）,2005年通过国家农作物品种审定委员会审定（国审稻2005020）。福建省长汀县地处闽西北,是闽西烤烟最大种植县,也是全省烤烟种植第2大县,常年烤烟种植面积4700hm^2,烟后稻面积4000hm^2。为选择优质、高产、抗病的烟后稻组合,充分发挥烟后稻生育期长、前作烤烟施肥量大、有机肥施用量多、土壤肥力好等优势,夺取烟后稻最高产量,取得最佳效益。长汀县于2005年引进D奇宝优527试种,通过3a在全县各乡镇烟后作栽培证明,表现适应范围广,增产潜力大,高产稳产,抗逆性强等优点。现将试种结果简述如下。     

Assessing climate change adaptations for community-scale water resources using a low-frequency weather generator

Paddy and Water Environment - Water adaptation strategies are necessary for decreasing climate change impacts on local societies. This study models a community water supply system as a distributed...     

Neuroprotective Metabolites from Vietnamese Marine Derived Fungi of Aspergillus and Penicillium Genera

Low molecular weight secondary metabolites of marine fungi Aspergillus flocculosus, Aspergillus terreus and Penicillium sp. from Van Phong and Nha Trang Bays (Vietnam) were studied and a number of polyketides, bis-indole quinones and terpenoids were isolated. The structures of the isolated compounds were determined by 1D and 2D NMR and HR-ESI-MS techniques. Stereochemistry of some compounds was established based on ECD data. A chemical structure of asterriquinone F (6) was thoroughly described for the first time. Anthraquinone (13) was firstly obtained from a natural source. Neuroprotective influences of the isolated compounds against 6-OHDA, paraquat and rotenone toxicity were investigated. 4-Hydroxyscytalone (1), 4-hydroxy-6-dehydroxyscytalone (2) and demethylcitreoviranol (3) have shown significant increasing of paraquat- and rotenone-treated Neuro-2a cell viability and anti-ROS activity.     

川西北高寒草地潜在生态草种营养成分的比较研究

对四川西北部高寒草甸草地的5种潜在生态草种不同生育期的营养成分进行了分析,结果表明,各草种不同生育期的粗水分、干物质、粗蛋白、粗脂肪、粗纤维、粗灰分等含量不同.其中赖草的干物质、蛋白质、粗脂肪、无氮浸出物和粗灰分含量显著高于川草Ⅰ号、川草Ⅱ号、垂穗鹅冠草和硬杆仲冰草等4种草.5种草不同生育期营养成分的分析结果表明,各草种分蘖期和孕穗期的粗水分、粗灰分、蛋白质、粗脂肪以及钙、磷含量明显高于结实期,而粗纤维、干物质和除赖草外的无氮浸出物含量则相反.表明在利用这些草种的过程中应抓好分蘖期的管理工作,以达到充分利用其饲用价值的目的.     

大学生篮球裁判员培养的目标层次与途径

篮球裁判员培养的目标设定对整个培训工作具有导向、控制、激励与评价等作用,体现篮球裁判员工作内容和等级分类管理的要求.在具体的培训实践中,应该按照循序渐进原则,充分考虑篮球裁判培养的整体性、阶段性、持续性、层次性和递进性等特点,制定针对不同等级裁判的培训目标,以对培训工作进行指导.通过课程教学、培训班、一级裁判员考核认证学习、社会实践等途径,培养优秀的大学生篮球裁判员.     

Competence of Aedes albopictus and Culex quinquefasciatus as vector of Dirofilaria immitis after blood meal with different microfilarial density

Aedes albopictus and Culex quinquefasciatus were fed canine blood with different microfilarial density of Dirofilaria immitis ranging from 2500 to 25,000 mff/ml. Larval development in these two mosquito species did not differ significantly. Although C. quinquefasciatus ingested more microfilariae, the number of larvae which developed in A. albopictus was invariably greater than in C. quinquefasciatus. Mortality of the engorged A. albopictus was significantly greater than that of C. quinquefasciatus, and higher microfilarial density raised the mortality in both species. The vector efficiency index of A. albopictus was greater than C. quinquefasciatus at all microfilarial densities, but its survival time was much reduced. Thus, dogs with low microfilarial density are implicated as the main source for the transmission of D. immitis from dogs to mosquitoes.