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1.
M Washizu K Kobayashi K Misaka T Hayashi G Kinoshita M Kondo S Aoki H Orima T Washizu 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(5):1051-1053
A 2-year-old female Japanese domestic cat weighing 3.6 kg was presented due to marked abdominal distention and was diagnosed as hepatic cyst. The bile acid concentration of the hepatic cyst was as low as the serum bile acid concentration. The membrane of the cysts was excised from the edge of the compressed hepatic tissue and no other surgical manipulation was performed. This surgical procedure is simple and quick compared to hepatic lobectomy, however, it is necessary to evaluate the fluid of the cyst before performing this procedure. 相似文献
2.
3.
Fujisawa H Suma K Origuchi K Kumagai H Seki T Ariga T 《Journal of agricultural and food chemistry》2008,56(11):4229-4235
This study verifies the instability of garlic ( Allium sativum L.)-derived allyl 2-propenylthiosulfinate (allicin) in various aqueous and ethanolic solutions as well as in vegetable oil through chemical and biological analyses performed simultaneously. Crushed fresh garlic cloves generated antibacterial activity and chemically detectable allicin, a major antibacterial principle, and both declined on a daily basis in aqueous and ethanolic solutions at room temperature, showing biological and chemical half-lives of about 6 and 11 days, respectively. Allicin was more stable in 20% alcohol than in water, but surprisingly unstable in vegetable oil, with an activity half-life 0.8 h, as estimated from its antibacterial activity toward Escherichia coli, and a chemical half-life of 3.1 h, based on chromatographic quantification. In alcoholic and aqueous extracts, the biological half-life of allicin tended to be longer than the chemical one, suggesting the occurrence of bioactive compounds other than allicin in the extracts. 相似文献
4.
Rodkhum C Hirono I Stork M Di Lorenzo M Crosa JH Aoki T 《Journal of fish diseases》2006,29(3):157-166
The genome of Vibrio anguillarum strain H775-3 was partially determined by a random sequencing procedure. A total of 2,300 clones, 2,100 from a plasmid library and 200 from a cosmid library, were sequenced and subjected to homology search by the BLAST algorithm. The total length of the sequenced clones is 1.5 Mbp. The nucleotide sequences were classified into 17 broad functional categories. Forty putative virulence-related genes were identified, 36 of which are novel in V. anguillarum, including a repeat in toxin gene cluster, haemolysin genes, enterobactin gene, protease genes, lipopolysaccharide biosynthesis genes, capsule biosynthesis gene, flagellar genes and pilus genes. 相似文献
5.
S D Hwang N Midorikawa P Punnarak Y Kikuchi H Kondo I Hirono T Aoki 《Journal of fish diseases》2012,35(12):927-934
RNA aptamers are artificial nucleic acids that specifically bind to a wide variety of targets. They are an effective tool for pharmaceutical research and development of antiviral agents. Here, we describe four Hirame rhabdovirus (HIRRV)‐RNA aptamers (H1, H2, H3 and H4) that we obtained from an in vitro process called the systematic evolution of ligands by exponential enrichment (SELEX). The HIRRV‐RNA aptamers specifically bind to HIRRV. Hirame natural embryo (HINAE) cells treated with virus and the RNA aptamer showed a decrease in appearance of cytopathic effect when compared with control (treated only with virus). Rhodovulum sulfidophilum was transformed with genes for the RNA aptamers, and the aptamers were detected in the culture medium, indicating that they were secreted from the cells. Thus, the recombinant R. sulfidophilum might be a powerful tool for the prevention of HIRRV in aquaculture. 相似文献
6.
Ahimbisibwe John Bosco Inoue Kousuke Shibata Toshiyuki Aoki Takahiko 《Fisheries Science》2010,76(2):389-394
The effect of bleeding on white muscle quality in amberjack and red sea bream was evaluated by measuring ATP-related compounds,
volatile basic nitrogen (VBN), and the trimethylamine (TMA) content. The freshness was evaluated by the K value, and the degree of spoilage was elucidated by VBN and TMA. ATP was rapidly converted to ADP and AMP in the muscle and
IMP was the main product of ATP degradation during iced storage. For both species, the IMP content was higher in the muscles
of fish that were bled than in those unbled during iced storage. Conversely, the K value and the levels of hypoxanthine (Hx) and VBN were higher in the muscle tissue of unbled fish than in the bled tissue
of both species. Similarly, the TMA content was higher in the unbled muscle tissues of both species after a week of storage. 相似文献
7.
Yamakawa K Aoki T Matsui M Yamada K Ishii M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(5):641-644
To establish a method to predict postpartum diseases using prepartum back fat thickness (BFT), the peripartum BFTs of 54 healthy multiparous cows before calving, which were diagnosed with postpartum displacement of the abomasum (DA), clinical ketosis or subclinical ketosis were compared with those of healthy cows from 8 weeks before the expected calving date to 8 weeks after calving. The peripartum BFTs of the cows with DA or clinical ketosis were significantly higher than those of healthy cows. The peripartum BFTs of the cows with subclinical ketosis were not significantly higher than those of the healthy cows. 相似文献
8.
Kuko Fuke Kana Takeshita Nanako Aoki Toshiyuki Fukuhara Mayumi Egusa Motoichiro Kodama Hiromitsu Moriyama 《Journal of General Plant Pathology》2011,77(4):248-252
Strains of the Japanese pear pathotype of Alternaria alternata were screened for double-stranded RNAs (dsRNAs). Four strains had several dsRNAs; strain N18 was associated with several
dsRNAs and had impaired growth phenotypes such as irregular mycelium and abnormal pigmentation. We isolated dsRNA-cured isolates
from strain N18 by single-conidium isolation. The dsRNA-cured isolates had recovered normal growth and pigmentation. Enlarged
vesicles were observed in mycelial cells of the original dsRNA-carrying N18 strain. DAPI nuclear staining revealed regression
of the nuclei in dsRNA-carrying N18 cells. These results indicate that the dsRNAs might have negative effects, such as apoptosis-like
cell death, on the host fungus. 相似文献
9.
Endo H Aoki R Taru H Kimura J Sasaki M Yamamoto M Arishima K Hayashi Y 《Anatomia, histologia, embryologia》2002,31(4):206-213
The masticatory muscles and their related structures of the skull were observed in the Indian gavial (Gavialis gangeticus), the false gavial (Tomistoma schlegelii), and the African slender-snouted crocodile (Mecistops cataphractus) to detail some morphological differences in comparison with the other crocodile species, and to compare and elucidate the functional strategy of themasticatory apparatus in these long-snouted species. The Musculus pterygoideus posterior was relatively smaller in the three species compared with many short-snouted crocodiles. It suggests that the masticatory power in fish-eating long-snouted species is not so high as in the short-snouted crocodiles, while the masticatory muscles were morphologically different among the three long-snouted species as follows. The M. pterygoideus posterior of the false gavial was extended in the lateral side of the lower jaw unlike the Indian gavial. The M. pseudotemporalis and the Fenestra supratemporalis were largely developed in the Indian gavial, however we suggest that the other two species possess the weak bundles in this muscle. The false gavial and the African slender-snouted crocodile have the pterygoid bone well-developed extending dorso-ventrally and it is suggested that the M. adductor mandibulae posterior attached to the pterygoid bone may be much larger than the Indian gavial. These data morphologically clarify the masticatory mechanism in the long-snouted crocodiles different from the short-snouted species, and demonstrate that the evolutional strategy to share the functional role in the masticatory muscles have been differently established between the Indian gavial and the other two species. We also obtained the morphological data in the fossil skull of the Machikane crocodile (Toyotamaphymeia machikanense) and concluded from the fossil characters that the considerable developments of the M.pterygoideus posterior and the M.pseudotemporalis in this species had not morphologically been consistent with both the Indian and false gavials. 相似文献
10.
Fujimoto Kiyoshi Miyagi Toyohiko Kikuchi Takao Kawana Toshio 《Mangroves and Salt Marshes》1996,1(1):47-57
Mangrove habitats on Kosrae are divided into three types, i.e., an estuary or delta type, a backmarsh or lagoon type and a coral reef or tidal-flat type. Most of the mangrove forests of Kosrae have been developed during the last 2000 years by accumulating mangrove peat with the gradual sea-level rise of 1 to 2 mm/yr except the landward part of the estuary or delta type. On the other hand, during the period of rapid sea-level rise of about 10 mm/yr between 4100 and 3700 yr B.P., the mangrove forests ceased peat accumulation and retreated landward. Until 3500 yr B.P., mangrove forests were distributed only in narrow bands in the inlets. Therefore, the critical rate of mangrove peat accretion with sea-level rise is estimated at more than 2 mm/yr and less than 10 mm/yr. If the anticipated sea-level rise exceeds this critical rate, all of the mangrove forests of Kosrae will retreat landward and reduce rapidly. 相似文献