全文获取类型
收费全文 | 310篇 |
免费 | 9篇 |
专业分类
林业 | 12篇 |
农学 | 12篇 |
62篇 | |
综合类 | 11篇 |
农作物 | 11篇 |
水产渔业 | 36篇 |
畜牧兽医 | 143篇 |
园艺 | 3篇 |
植物保护 | 29篇 |
出版年
2023年 | 2篇 |
2022年 | 8篇 |
2021年 | 10篇 |
2020年 | 3篇 |
2019年 | 3篇 |
2018年 | 4篇 |
2017年 | 11篇 |
2016年 | 7篇 |
2015年 | 11篇 |
2014年 | 19篇 |
2013年 | 18篇 |
2012年 | 15篇 |
2011年 | 16篇 |
2010年 | 5篇 |
2009年 | 12篇 |
2008年 | 27篇 |
2007年 | 22篇 |
2006年 | 22篇 |
2005年 | 23篇 |
2004年 | 24篇 |
2003年 | 17篇 |
2002年 | 21篇 |
2001年 | 4篇 |
2000年 | 4篇 |
1999年 | 4篇 |
1998年 | 3篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1992年 | 1篇 |
1990年 | 1篇 |
排序方式: 共有319条查询结果,搜索用时 15 毫秒
1.
Ookawa K Mochizuki K Sugimoto Y Suzuki T Hashizume M Kitagawa T Matumoto T Ito C Yokogoshi H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(1):49-54
Lipid extracted from the ovary of skipjack tuna by the method that we developed is rich in phospholipid-type docosahexaenoic acid. The ovary lipid of skipjack tuna (OLS) was studied for its anti-stress activity in male Wistar rats, focusing on stress-related blood components: recovery from stress was examined after application of water immersion restraint stress. As a result, serum corticosterone (CORT) secretion was inhibited and decreased rapidly after stress application in rats given OLS compared with control rats. As CORT acts as a glucocorticoid, non-esterified fatty acid (NEFA) is expected to increase by stress application. However, the concentration tended to be lower in rats given OLS than in control rats. With respect to OLS concentration, OLS increased serum dehydroepiandrosterone, secretion concentration-dependently. In addition, as with the recovery study, it tended to inhibit the increase in NEFA. These results indicate that OLS may have an anti-stress activity against acute stress. 相似文献
2.
Isao Tada Minoru Motoki Nobuyoshi Takahashi Tetsuji Miyata Tomoko Takechi Toshiro Uchida Yasushi Takagi 《Pest management science》1996,48(2):165-173
A series of novel 4,5-dihydropyrazole-5-thiones (DHPs) was synthesised by treating the corresponding dihydropyrazolones with ‘Lawesson’s reagent and evaluated for miticidal activity against two-spotted spider mites (Tetranychus urticae Koch). Of these, 3-(4-chlorophenyl)-4,4-dimethyl-1-phenyl-4,5-dihydropyrazole-5-thione, 3-(4-chlorophenyl)-4-ethyl-4-methyl-1-phenyl-4,5-dihydropyrazole-5-thione, 3-(4-chlorophenyl)-1-phenyl-4,5-dihydropyrazole-5-thione-4-spirocyclopentane and 4,4-dimethyl-1-phenyl-3-(4-trifluoromethyl-phenyl)-4,5-dihydropyrazole-5-thione were highly active (pEC50>4·0) and were more effective than the miticide dicofol (pEC50=3·879), which has traditionally been used for the control of phytophagous mites. Structure–activity relationship (SAR) studies were performed on each position of the pyrazole ring of DHPs. The results indicated that the unsubstituted phenyl, 4-substituted phenyl and thioxo groups on the 1-, 3- and 5-positions of DHPs respectively were required for activity. Quantitative SAR studies using physicochemical parameters of substituents and the capacity factor k′ as a hydrophobicity index suggested that: (a) the activities of all types of DHPs examined were mainly dominated by hydrophobicity, (b) the bulkiness of 4-substituents of the 3-phenyl ring favoured the activity and (c) the log k′ optimum for all DHPs was 1·675, equivalent to a log Pow value of c. 5·0. 相似文献
3.
Study on the glass transition for several processed fish muscles and its protein fractions using differential scanning calorimetry 总被引:1,自引:0,他引:1
ABSTRACT: The glass transition behavior of processed fish muscles (bonito, tuna, mackerel, sea bream, cod) and its muscle protein fractions (sarcoplasmic and myofibrillar proteins) were studied using differential scanning calorimetry. Each dried processed fish muscle and the extracted protein fractions showed clear glass transition phenomenon. The T g values of muscles and myofibrillar proteins from red muscle fishes tended to be lower than those from white muscle fishes though there was no difference on T g of sarcoplasmic proteins. The T g value of whole muscle was considerably lower than that of extracted protein fractions because of the plasticizing effects of low molecular weight materials contained in the muscle. 相似文献
4.
Haruka KATAOKA Nanami KOITA Natsuko ITO KONDO Hiroshi C. ITO Momoko NAKAJIMA Kunikazu MOMOSE Hiroko IIMA Tomoo YOSHINO Tomoko AMANO Takio KITAZAWA Daiji ENDOH Nobuyoshi NAKAJIMA Hiroki TERAOKA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(3):358
The red-crowned crane Grus japonensis in Hokkaido, Japan forms a closed population as a residence that is independent of the mainland population. Based on observations of a limited number of individuals as well as cranes in captivity, red-crowned cranes are omnivores and eat fish, worms, insects and plants in their own territories except in winter, when they are fed with dent corn that is supplied in eastern Hokkaido. DNA metabarcoding based on high throughput sequencing was carried out using universal primer sets for cytochrome oxidase subunit I gene. Feces from 27 chicks collected in June and July in the period from 2016 to 2018 and intestinal contents from 33 adult and subadult cranes that were found dead almost throughout year in 2006–2013 in the field in eastern Hokkaido were used. Although compositions varied considerably in the cranes, both insects and fish were found in adults and subadults to the same extents, while insects were predominant in chicks. Both insects and fish were detected in all seasons for adults and subadults. Horse flies, scarab beetles and weevils accounted for the most of the insects regardless of the life stage. Dace, stickleback, flatfish and sculpin were the major fish species in adults, while chicks ate almost only stickleback. The results provide the first comprehensive data on carnivorous diets in wild red-crowned cranes in eastern Hokkaido as basis for conservation of red-crowned cranes, for which the life style and area continue to change. 相似文献
5.
Tomoko ITAHASHI Toshinori OIKAWA Takashi NUMABE 《The Journal of reproduction and development》2022,68(1):74
This study was conducted to examine the effects of adding glutathione (1 mM) to media used for sperm washing and in vitro fertilization (IVF) on the improvement of early development of embryos produced using cryopreserved spermatozoa of the less IVF-competent bull (the one considered unqualified as spermatozoa supplier for the production of bovine blastocysts using IVF). The cryopreserved spermatozoa of this bull were characterized by normal motility and lower ATP content and blastocyst productivity than those of IVF-competent bulls. The addition of glutathione to the sperm washing medium was more effective in improving the productivity of blastocysts and ATP content than the addition of glutathione to the IVF medium or no glutathione addition at all (control). These results suggest that this simple method may be used to improve the potential of cryopreserved spermatozoa of less IVF-competent bulls to fertilize oocytes in vitro and to induce normal embryonic development after fertilization. 相似文献
6.
Effects of feeding and animal performance on nitrogen, phosphorus and potassium excretion by Holstein cows 总被引:1,自引:0,他引:1
Hideki KOJIMA Shin-ichi KUME Kazuhisa NONAKA Tomoko OSHITA Takaharu KOZAKAI Hiroyuki HIROOKA 《Animal Science Journal》2005,76(2):139-145
Thirty‐four Holstein dry cows and 16 lactating cows were used in balance trials to identify the effects of feeding and animal performance on nitrogen (N), phosphorus (P) and potassium (K) excretion by dairy cows, and to develop prediction models for these excreta. Orchard grass silage, corn silage, alfalfa silage or timothy hay were offered to dry cows. Orchard grass silage or alfalfa silage, and concentrates were offered to lactating cows. In the statistical analysis, the independent variables were bodyweight (kg), dry matter (DM) intake (kg/day), milk yield (for lactating cows only, kg/day), water intake (free water plus water in feed, kg/day), intake (g/day) of N, P and K and dietary contents (% of DM) of crude protein, P and K. The dependent variables were N, P and K excretion (g/day) in feces and urine. In both dry and lactating cows, intake of N, P and K had large effects on corresponding excretion. The results indicated that a decrease in the intake of N, P and K could decrease the corresponding excretion. Further research by path analysis showed that K intake positively affected urinary N excretion in dry cows indirectly, through water intake and urine volume. 相似文献
7.
Sasaki Y Fujisawa T Ogikubo K Ohzono T Ishihara K Takahashi T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(1):129-131
To isolate Campylobacter spp., the feces of healthy cattle, pigs, and broilers were examined between June 1999 and January 2000. Campylobacter lanienae strains were isolated from the feces of healthy pigs, but not from the feces of cattle or broilers. In six C. lanienae isolates, there was only 21-38% DNA-DNA homology to Campylobacter hyointestinalis subsp. lawsonii strain NCTC 12901. Thus, the primary host of C. lanienae is likely to be the pig and C. lanienae appears to be a species distinct from C. hyointestinalis subsp. lawsonii. In addition, an intervening sequence of 226 bp in the 16S rRNA gene was found in four isolates. 相似文献
8.
Kentaro Okuno Tomoko Hama Minoru Takeshita Naruto Furuya Yoichi Takanami 《Journal of General Plant Pathology》2003,69(2):138-142
A potyvirus, for which the name Japanese hornwort mosaic virus (JHMV) is proposed, was isolated from Japanese hornwort plants
(Cryptotaenia japonica) with mosaic disease symptoms. The virus was used to inoculate mechanically 34 plants belonging to 33 species of 10 families.
Of these species seven from two families were infected. Faint chlorotic spots appeared on the inoculated leaves of Chenopodium quinoa and C. amaranticolor, but no systemic infection occurred in these plants. JHMV systemically infected only Umbelliferae plants; they did not infect
26 other species in eight families. JHMV was transmitted in a nonpersistent manner by aphids (Myzus persicae). The virus was a flexuous rod-shaped particle about 750 nm in length. Sequencing the nucleotides in the 3′ terminal region
of JHMV revealed that the coat protein contains 280 amino acids with a molecular mass of 32.2 kDa. The nucleotide sequence
of the coat protein of JHMV had the highest similarity with that of Zantedeschia mosaic virus (83.3%) compared to those of
other potyviruses (57.0%–64.9%). An antiserum against JHMV reacted strongly with JHMV and weakly with Potato virus Y. These results indicate that JHMV is a new potyvirus.
Received: September 9, 2002 / Accepted: November 7, 2002
RID="*"
ID="*" The nucleotide sequence determined in this work appears in the DDBJ/EMBL/GenBank nucleotide sequence databases with
the accession number AB081518 相似文献
9.
10.
Imai K Ishihara R Jayawardane GW Nishimori K Nishimori T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(10):953-956
Three different polymerase chain reaction (PCR) protocols were evaluated for their ability to detect bovine herpesvirus 2 (BoHV-2): single-step PCR with 3 reaction stages (denaturation, annealing and extension), 2 reaction stages (denaturation and annealing/extension; shuttle PCR), and semi-nested PCR with 3 reaction stages. All the PCR protocols showed the same sensitivity (detection limit of 0.4 TCID(50)). A non-specific band sometimes appeared in mock cell DNA at annealing temperatures below 64 degrees C. The shuttle PCR was found to be superior to the other protocols under consideration because of the speed of its application. Furthermore, no non-specific band was detected in DNAs of eight other DNA viruses. Thus, the shuttle PCR seems to be an excellent diagnostic tool for BoHV-2 infections. 相似文献