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排序方式: 共有518条查询结果,搜索用时 15 毫秒
1.
2.
Takeshi SHINOGI Tomoko SUZUKI Yoshihiro NARUSAKA Pyoyun PARK 《Journal of General Plant Pathology》2002,68(1):38-45
The rapid generation of reactive oxygen species (ROS), called the oxidative burst, is one of the earliest host responses to
pathogen infection or elicitor treatments. Therefore, we looked for the induction of ROS generation in Japanese pear leaves
by the host-specific toxin, AK-toxin I using a cytochemical method for detecting H2O2. A small amount of non-specific generation of H2O2 was found in the cell walls in toxin- and water-treated susceptible and resistant leaves. Thus, the generation of H2O2 at cell walls appears to be caused by wounding stress during sampling. Specific generation of ROS, however, was found only
in the membrane fragments and extended desmotubules characteristic of modified sites of the plasma membrane in the toxin-treated
susceptible leaves. In addition, generation of H2O2 at plasma membranes was observed with higher frequency in toxin-treated susceptible leaves. This result indicates that the
H2O2 generation was associated with damaged sites in the plasmalemma after toxin treatment and perhaps with the formation of membrane
fragments from altered portions of the invaginated plasma membrane.
Received 21 September 2001/ Accepted in revised form 25 October 2001 相似文献
3.
Takeshi Shinogi Tomoko Suzuki Takayuki Kurihara Yoshihiro Narusaka Pyoyun Park 《Journal of General Plant Pathology》2003,69(1):7-16
Reactive oxygen species (ROS) generation was examined in the interaction of Alternaria alternata Japanese pear pathotype and host plants using three methods: nitro blue tetrazolium (NBT) method for microscopic detection
of O2
−, diaminobenzidine (DAB) methods for microscopic detection of H2O2, and cerium chloride methods for ultrastructural detection of H2O2. ROS generation was detected by NBT and DAB methods at appressoria on leaves of susceptible cultivars and heat-shocked leaves
of resistant cultivars but not in leaves of resistant cultivars. Ultrastructural detection by the cerium chloride method identified
ROS generation at cell walls of appressoria and penetration pegs in susceptible, resistant leaves and heat-shocked leaves.
These differences in the ultrastructural and microscopic data in resistant areas were due to the restriction of ROS generation
in limited areas, the side facing the plant surface, of appressoria and penetration pegs. Therefore, ROS generation was apparently
induced regardless of the resistance or susceptibility of the cultivar with the difference being in the volumes generated.
After evaluating the pathological role of ROS generation in fungal structures, such generation was found to be associated
with early penetration of cell walls in pear plants. Additionally, ROS generation in plants was also found in degrading pectin
layers near infected hyphae and in plasma membrane modification sites in susceptible leaves but not in resistant leaves. ROS
generation in susceptible leaves might be accompanied with plasma membrane damage, although the role of ROS generation in
the pectin layers is not clear. ROS generation in both fungal and plant cells during their interaction was likely associated
with the expression of susceptibility.
Received: June 3, 2002 / Accepted: July 31, 2002 相似文献
4.
5.
Taiki KIDA Arisa YAMAZAKI Koji KOBAYASHI Tatsuro NAKAMURA Takayuki NAKAGAWA Ryohei NISHIMURA Takahisa MURATA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(5):644
Polyunsaturated fatty acids, including arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA), are converted to hundreds of lipid mediators by cyclooxygenases (COX), lipoxygenases (LOX), and cytochrome P450 (CYP), or through non-enzymatic processes, and they reflect inflammatory states of the body. We comprehensively analyzed lipid metabolites in dog urine using a liquid chromatograph-mass spectrometry (LC-MS/MS) to describe their metabolic characteristics. We detected 31 AA-derived metabolites, four EPA-derived metabolites, and a DHA-derived metabolite in all urine samples. Among AA-derived metabolites, 15, 5, 3, and 8 were generated by COX, LOX, CYP, and non-enzymatic oxidation respectively. This study will be the first step to use profiles of urinary lipid metabolites for better understanding and diagnosis of canine diseases. 相似文献
6.
7.
Ookawa K Mochizuki K Sugimoto Y Suzuki T Hashizume M Kitagawa T Matumoto T Ito C Yokogoshi H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(1):49-54
Lipid extracted from the ovary of skipjack tuna by the method that we developed is rich in phospholipid-type docosahexaenoic acid. The ovary lipid of skipjack tuna (OLS) was studied for its anti-stress activity in male Wistar rats, focusing on stress-related blood components: recovery from stress was examined after application of water immersion restraint stress. As a result, serum corticosterone (CORT) secretion was inhibited and decreased rapidly after stress application in rats given OLS compared with control rats. As CORT acts as a glucocorticoid, non-esterified fatty acid (NEFA) is expected to increase by stress application. However, the concentration tended to be lower in rats given OLS than in control rats. With respect to OLS concentration, OLS increased serum dehydroepiandrosterone, secretion concentration-dependently. In addition, as with the recovery study, it tended to inhibit the increase in NEFA. These results indicate that OLS may have an anti-stress activity against acute stress. 相似文献
8.
Isao Tada Minoru Motoki Nobuyoshi Takahashi Tetsuji Miyata Tomoko Takechi Toshiro Uchida Yasushi Takagi 《Pest management science》1996,48(2):165-173
A series of novel 4,5-dihydropyrazole-5-thiones (DHPs) was synthesised by treating the corresponding dihydropyrazolones with ‘Lawesson’s reagent and evaluated for miticidal activity against two-spotted spider mites (Tetranychus urticae Koch). Of these, 3-(4-chlorophenyl)-4,4-dimethyl-1-phenyl-4,5-dihydropyrazole-5-thione, 3-(4-chlorophenyl)-4-ethyl-4-methyl-1-phenyl-4,5-dihydropyrazole-5-thione, 3-(4-chlorophenyl)-1-phenyl-4,5-dihydropyrazole-5-thione-4-spirocyclopentane and 4,4-dimethyl-1-phenyl-3-(4-trifluoromethyl-phenyl)-4,5-dihydropyrazole-5-thione were highly active (pEC50>4·0) and were more effective than the miticide dicofol (pEC50=3·879), which has traditionally been used for the control of phytophagous mites. Structure–activity relationship (SAR) studies were performed on each position of the pyrazole ring of DHPs. The results indicated that the unsubstituted phenyl, 4-substituted phenyl and thioxo groups on the 1-, 3- and 5-positions of DHPs respectively were required for activity. Quantitative SAR studies using physicochemical parameters of substituents and the capacity factor k′ as a hydrophobicity index suggested that: (a) the activities of all types of DHPs examined were mainly dominated by hydrophobicity, (b) the bulkiness of 4-substituents of the 3-phenyl ring favoured the activity and (c) the log k′ optimum for all DHPs was 1·675, equivalent to a log Pow value of c. 5·0. 相似文献
9.
Shohei KOBAYASHI Natsuko KAWANO Kenji MIYADO Ryo OHTA Takahiro AKIMOTO Taichi HATAKEYAMA Maiko KAWAGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(1):153
Tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) is widely used as a flame retardant and is known to exhibit anti-androgenic effects in vitro and in vivo. To assess the reproductive toxicity potency of TDCIPP, we investigated the effects of 7 days of TDCIPP oral administration on epididymal sperm motion and concentration in adult male Wistar–Imamichi rats. Thirty-five days after the final administration, sperm parameters were evaluated by computer-assisted sperm analysis. Results showed that sperm swimming progression and vigor and sperm concentration in TDCIPP-treated rats were unexpectedly higher than those in control rats. TDCIPP did not significantly affect the percentage of motile sperms or sperm swimming pattern. These results contribute to the understanding of the biological effects of TDCIPP. 相似文献
10.
Study on the glass transition for several processed fish muscles and its protein fractions using differential scanning calorimetry 总被引:1,自引:0,他引:1
ABSTRACT: The glass transition behavior of processed fish muscles (bonito, tuna, mackerel, sea bream, cod) and its muscle protein fractions (sarcoplasmic and myofibrillar proteins) were studied using differential scanning calorimetry. Each dried processed fish muscle and the extracted protein fractions showed clear glass transition phenomenon. The T g values of muscles and myofibrillar proteins from red muscle fishes tended to be lower than those from white muscle fishes though there was no difference on T g of sarcoplasmic proteins. The T g value of whole muscle was considerably lower than that of extracted protein fractions because of the plasticizing effects of low molecular weight materials contained in the muscle. 相似文献