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排序方式: 共有421条查询结果,搜索用时 15 毫秒
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2.
Do Thi Thanh HUONG Vidya JAYASANKAR Safiah JASMANI Hisako SAIDO-SAKANAKA Andrew J. WIGGINTON Marcy N. WILDER 《Fisheries Science》2004,70(3):518-520
3.
Nadezhda E. Ustyuzhanina Maria I. Bilan Andrey S. Dmitrenok Eugenia A. Tsvetkova Sofya P. Nikogosova Cao Thi Thuy Hang Pham Duc Thinh Dinh Thanh Trung Tran Thi Thanh Van Alexander S. Shashkov Anatolii I. Usov Nikolay E. Nifantiev 《Marine drugs》2022,20(6)
Fucosylated chondroitin sulfates (FCSs) FCS-BA and FCS-HS, as well as fucan sulfates (FSs) FS-BA-AT and FS-HS-AT were isolated from the sea cucumbers Bohadschia argus and Holothuria (Theelothuria) spinifera, respectively. Purification of the polysaccharides was carried out by anion-exchange chromatography on DEAE-Sephacel column. Structural characterization of polysaccharides was performed in terms of monosaccharide and sulfate content, as well as using a series of non-destructive NMR spectroscopic methods. Both FCSs were shown to contain a chondroitin core [→3)-β-d-GalNAc-(1→4)-β-d-GlcA-(1→]n bearing sulfated fucosyl branches at O-3 of every GlcA residue in the chain. These fucosyl residues were different in pattern of sulfation: FCS-BA contained Fuc2S4S, Fuc3S4S and Fuc4S at a ratio of 1:8:2, while FCS-HS contained these residues at a ratio of 2:2:1. Polysaccharides differed also in content of GalNAc4S6S and GalNAc4S units, the ratios being 14:1 for FCS-BA and 4:1 for FCS-HS. Both FCSs demonstrated significant anticoagulant activity in clotting time assay and potentiated inhibition of thrombin, but not of factor Xa. FS-BA-AT was shown to be a regular linear polymer of 4-linked α-L-fucopyranose 3-sulfate, the structure being confirmed by NMR spectra of desulfated polysaccharide. In spite of considerable sulfate content, FS-BA-AT was practically devoid of anticoagulant activity. FS-HS-AT cannot be purified completely from contamination of some FCS. Its structure was tentatively represented as a mixture of chains identical with FS-BA-AT and other chains built up of randomly sulfated alternating 4- and 3-linked α-L-fucopyranose residues. 相似文献
4.
Chang‐Hoon Shin Hien Thi Dieu Bui Samad Rahimnejad Ji‐Hoon Cha Byung‐Woo Yoo Bo‐Kyeun Lee Hyung‐Jin Ahn Soo‐Il Choi Yun‐Jeong Choi Yong‐Ho Park Jeong‐Dae Kim Kyeong‐Jun Lee 《Journal of the World Aquaculture Society》2014,45(3):258-268
This study was conducted to evaluate the effects of dietary supplementation of Barodon, an anionic alkali mineral complex, on growth, feed utilization, humoral innate immunity and disease resistance of olive flounder. A basal experimental diet was used as a control and supplemented with 0.1, 0.2, 0.3, 0.4, or 0.5% Barodon. Triplicate groups of fish (26.4 ± 0.2 g) were fed one of the diets to apparent satiation twice daily for 10 wk. The growth performance was enhanced (P < 0.05) linearly and quadratically in fish fed diets containing Barodon compared with that in fish fed the control. Feed utilization was significantly improved by Barodon supplementation. Serum lysozyme and antiprotease activities were increased quadratically in Barodon fed groups. Also, significantly higher superoxide dismutase activity was found in Barodon‐fed fish. Dietary supplementation of 0.1–0.3% Barodon resulted in significant enhancement of fish disease resistance against Streptococcus iniae. The findings in this study indicate that dietary supplementation of Barodon can enhance growth, feed utilization, innate immunity, and disease resistance of olive flounder and that the optimum level seems to be 0.1% in diets. 相似文献
5.
Dominant sources of dietary carbon and nitrogen for shrimp reared in extensive rice-shrimp ponds 总被引:1,自引:0,他引:1
Michele A Burford Nigel P Preston Truong Hoang Minh Tran Thi Tuyet Hoa Stuart E Bunn & Vanessa M Fry 《Aquaculture Research》2004,35(2):194-203
Stable isotope analysis was used to determine the sources of dietary nitrogen (N) and carbon (C) for shrimp during the rearing phase in extensive rice‐shrimp ponds in My Xuyen and Gia Rai districts, Vietnam. Farm‐made feed was added as a food source in shrimp ponds in My Xuyen district, and based on stable isotope analyses, was generally a poor dietary source. The commercial formulated feed used in Gia Rai also appeared to contribute little directly to the nutritional needs of the shrimp. In contrast, the natural biota in all ponds appeared to contribute substantially. In particular, biota from beam trawls and benthic organic matter were the most likely sources of nutrition in My Xuyen ponds, while benthic organic matter was the main source in Gia Rai ponds. δ15N ratios in the natural biota in My Xuyen farms decreased over the growing season, suggesting increased N fixation in the case of the benthic organic matter reaching values as low as 1‰. This suggests N‐limitation in the ponds and that natural biota become increasingly dependent on N fixed by algae and/or other microorganisms. There is the potential to promote the growth of the plankton and hence, the other natural biota, by the judicious addition of fertilizer. 相似文献
6.
Thi Thanh Nhan Pham Thi Ngoc Lan Nguyen Thi Ha Bui Huu Quan Nguyen Thi Tam Nguyen Van Son Le 《The Journal of Horticultural Science and Biotechnology》2019,94(5):591-598
Catharanthus roseus contains vincristine and vinblastine, which are outstanding drugs for cancer. In the biosynthetic pathways of terpenoid indole alkaloids (TIAs) in C. roseus, deacetylvindoline 4-O-acetyltransferase (DAT) is a key enzyme that catalyses the last reaction of vindoline biosynthesis to form vinblastine and vincristine. In this study, the CrDAT transgene was transferred into the periwinkle by Agrobacterium-mediated transformation and generated transgenic periwinkle lines with an increase in vincristine accumulation. The C. roseus DAT gene was introduced into C. roseus plants and it was confirmed that CrDAT was successfully transferred into the genome of periwinkle plants and efficiently translated to synthesise recombinant DAT protein. Four transgenic periwinkle lines in T1 generation, T1-1, T1-3, T1-6, and T1-7, expressed recombinant DAT protein with the total protein content in the range of 2.86 μg.mg?1 to 5.12 μg.mg?1. Moreover, the vincristine contents of four transgenic lines increased by 1.63?2.48-fold compared to non-transgenic plants, ranging from 6.91 µg.g?1 (fresh weight) to 10.53 µg.g?1 (fresh weight). The T1-1 line had the highest vincristine content. Hence, the overexpression of the recombinant DAT protein can improve the vincristine accumulation of transgenic C. roseus plants.Abbreviation: CrDAT - Catharanthus roseus Deacetylvindoline-4-O-Acetyl Transferase; D4H - Deacetoxyvindoline 4-hydroxylase; ELISA - Enzyme-Linked Immunosorbent Assay Monoterpene indole alkaloid; T0, T1 - Generations of transgenic plants; TIAs - Terpenoid indole alkaloids; WT- The wild-type tobacco plants (non transgenic plant); 35S - Cauliflower mosaic virus 35S promoter 相似文献
7.
辐射花粉授粉是获得单倍体的有效途径。本研究旨在探讨该途径中影响单倍体再生率的关键因素。本试验利用60Coγ对不同黄瓜材料进行不同剂量的辐射处理,系统研究了辐射剂量、花粉供体基因型、母本基因型对诱导黄瓜单倍体植株再生率的影响。结果表明:3个花粉供体基因型(5211、CC3、南抗一号)花粉经150 Gy辐射处理后,花粉供体基因型5211诱导的植株再生率、单倍体再生率均高于花粉供体基因型CC3和南抗一号。花粉供体在辐射剂量150 Gy辐射处理下较250 Gy及350 Gy更有利于诱导植株再生,再生率最高可达3.92%,单倍体再生率为1.96%。10个母本基因型中6个母本基因型没有诱导出再生植株,占母本基因型总量的60%;4个母本基因型诱导出再生植株,占母本基因型总量的40%,单倍体诱导率为0.26~0.54。 相似文献
8.
Oanh Nguyen Cong Taminiau Bernard Dang Pham Kim Luc Do Duc Moula Nassim Huyen Nguyen Thi Thinh Nguyen Hoang Daube Georges Bindelle Jrme Ton Vu Dinh Jean‐Luc Hornick 《Animal Science Journal》2019,90(8):948-960
The aim of this study was to evaluate the effects of diets containing rice distillers’ by‐product (RDP) on growth performance, carcass characteristics, meat quality, and gut microbiota of fattening pigs. Twenty‐four crossbred finishing pigs (Duroc × Landrace × Yorkshire), 56.9 ± 3.1 kg initial body weight, were randomly allocated to three groups. For 56 days, pigs were fed one of three diets including RDP0 (control), RDP15 (15% RDP in DM), and RDP30 (30% RDP in DM). With RDP level in diet, average daily gain and backfat thickness linearly increased (p < 0.05), and drip loss tended to increase (p ≤ 0.08). In addition, 16S ribosomal RNA gene amplicon profiling showed that RDP was associated with modulation of colonic microbiota composition, especially at family and genus levels. Relative abundance of Porphyromonadaceae and Erysipelotrichaceae families in colonic digesta increased with inclusion of RDP, while that of Enterobacteriaceae decreased. The proportion of genera unclassified Erysipelotrichaceae, and Butyrivibrio increased as inclusion of RDP. These results indicate that up to 30% inclusion in diet of finishing pigs, RDP can modulate colonic microbiota composition, and induces an improvement of animal growth and fat deposition. 相似文献
9.
基于SRTM_DEM的泾河流域特征信息提取研究 总被引:2,自引:0,他引:2
流域特征信息的提取对于水文地理信息分析、模拟技术和水文模型建立具有重要意义。基于STRM_DEM数据,在ArcGIS9.3环境下,提取泾河流域坡度、坡向等地形特征,以及河网、流域边界和面积等水文要素,并划分子流域。分析表明,当闽值取5000(40.5km^2)时,生成的流域河网能够较好地反映该地区水系。根据河网分级、低级集水区域生成结果和流域地形特征,可划分为32个子流域,为流域分布式水文模型的构建提供了基础地理信息。SRTM_DEM提取泾河和部分支流流域面积误差均较小,说明提取结果与实际非常接近。结果表明,SRTM_DEM数据和ArcGIS9.3提取流域特征信息方便快捷,具有较高的精度和准确性,应用前景广阔。 相似文献
10.
Downy mildews cause considerable damage to maize (Zea mays L.) worldwide, particularly in the tropical Asia. We have evaluated a set of 42 tropical/sub-tropical maize inbred lines
developed in different countries in Asia (India, Thailand and Philippines), and Mexico, for analysing the genetic variability
for resistance to sorghum downy mildew [Peronosclerospora sorghi; SDM] and Rajasthan downy mildew [P. heteropogoni; RDM]. Experiments were carried out in replicated trials under artificial infection in field conditions against SDM and RDM
at Mandya in Karnataka, India, and Udaipur in Rajasthan, India, respectively, during 1999 and 2000. The study resulted in
identification of five inbred lines offering consistent and strong resistance to both SDM and RDM, while several inbred lines
revealed resistance only to RDM. It was also revealed that the SDM-resistant inbreds are invariably resistant to RDM, while
the RDM-resistant inbreds might show differential responses to the SDM. The maize inbred lines identified in this study with
broad-spectrum resistance to downy mildews could be potentially useful for basic and applied research work on downy mildews
in tropical Asia.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献