全文获取类型
收费全文 | 75篇 |
免费 | 12篇 |
专业分类
林业 | 3篇 |
农学 | 2篇 |
基础科学 | 1篇 |
12篇 | |
综合类 | 3篇 |
农作物 | 4篇 |
水产渔业 | 20篇 |
畜牧兽医 | 37篇 |
园艺 | 1篇 |
植物保护 | 4篇 |
出版年
2022年 | 1篇 |
2021年 | 3篇 |
2020年 | 4篇 |
2019年 | 4篇 |
2018年 | 7篇 |
2017年 | 5篇 |
2016年 | 1篇 |
2015年 | 1篇 |
2014年 | 4篇 |
2013年 | 9篇 |
2012年 | 7篇 |
2011年 | 6篇 |
2010年 | 4篇 |
2009年 | 5篇 |
2008年 | 5篇 |
2007年 | 2篇 |
2006年 | 1篇 |
2005年 | 4篇 |
2004年 | 1篇 |
2003年 | 2篇 |
2002年 | 2篇 |
2001年 | 3篇 |
2000年 | 2篇 |
1998年 | 2篇 |
1991年 | 1篇 |
1987年 | 1篇 |
排序方式: 共有87条查询结果,搜索用时 31 毫秒
1.
Abugoch LE Romero N Tapia CA Silva J Rivera M 《Journal of agricultural and food chemistry》2008,56(12):4745-4750
The amino acid composition and the physicochemical and functional properties of quinoa protein isolates were evaluated. Protein isolates were prepared from quinoa seed by alkaline solubilization (at pH 9, called Q9, and at pH 11, called Q11) followed by isoelectric precipitation and spray drying. Q9 and Q11 had high levels of essential amino acids, with high levels of lysine. Both isolates showed similar patterns in native/SDS-PAGE and SEM. The pH effect on fluorescence measurements showed decreasing fluorescence intensity and a shift in the maximum of emission of both isolates. Q9 showed an endotherm with a denaturation temperature of 98.1 degrees C and a denaturation enthalpy of 12.7 J/g, while Q11 showed no endotherm. The protein solubility of Q11 was lower than that of Q9 at pH above 5.0 but similar at the pH range 3.0-4.0. The water holding capacity (WHC) was similar in both isolates and was not affected by pH. The water imbibing capacity (WIC) was double for Q11 (3.5 mL of water/g isolate). Analysis of DSC, fluorescence, and solubility data suggests that there is apparently denaturation due to pH. Some differences were found that could be attributed to the extreme pH treatments in protein isolates and the nature of quinoa proteins. Q9 and Q11 can be used as a valuable source of nutrition for infants and children. Q9 may be used as an ingredient in nutritive beverages, and Q11 may be used as an ingredient in sauces, sausages, and soups. 相似文献
2.
Zaragoza C Barrera R Centeno F Tapia JA Durán E González M Mañé MC 《Veterinary research》2003,34(2):137-151
Canine leishmaniasis is an endemic disease in the Mediterranean area caused by the protozoan Leishmania infantum, which usually produces renal failure. Sodium dodecyl sulphate polyacrylamide gel electrophoresis and Western blot using antibodies to IgG and IgA from dogs were carried out in the urine of 22 dogs with leishmaniasis diagnosed by ELISA and confirmed by PCR, and 20 healthy dogs. The results were compared to renal function laboratory tests and to those from a histopathological study of the kidneys from sick animals that died naturally or were euthanized. Five different bands with molecular weights ranging from 10 to 110 kDa were obtained from the electrophoresis of the urine of healthy dogs. 33.5% of total proteins corresponded to low molecular weight proteins and the other proteins had middle and high molecular weights. However, in the group with leishmaniasis, a maximum of 11 different bands with molecular weights ranging from 10 kDa to 150 kDa were displayed in the electrophoresis of the urine. The urine electrophoretic pattern in the sick dogs was classified as mixed (proteins with high and low molecular weights) because low molecular weight proteins made up 57.9% and the rest of the proteins had middle and high molecular weights. In Western blot, none of the healthy dogs showed excretion of IgG and/or IgA, whereas IgG and IgA were detected in the Western blot of urine of 68% and 55% respectively of dogs with leishmaniasis. The results obtained in the leishmaniasis group agreed with glomerular and tubular damage, which were confirmed by the histopathological findings. 相似文献
3.
Castro E Soraci A Fogel F Tapia O 《Journal of veterinary pharmacology and therapeutics》2000,23(5):265-271
The chiral inversion process is a characteristic metabolic pathway for different aryl-2-propionic acids or profens. Important variations have been observed between these individual compounds as well as between animal species. In this study, R(-) fenoprofen [R(-)FPF] and R(-) ketoprofen [R(-) KTF] were used to investigate their comparative stereoconversion in cats. After intravenous (i.v.) administration of R(-) FPF, the percentage of chiral inversion was 93.20+/-13.70%. A highly significant correlation (r: 0.978) was observed between the clearance of R(-) FPF and the chiral inversion process. After i.v. administration of R(-) KTF, the percentage of inversion was only 36.73+/-2.8%. No correlation between the clearance of R(-) KTF and this process was observed. R(-) FPF was metabolized by the pathways of thioesterification - chiral inversion processes. For R(-) KTF, the competitive metabolic pathways, glucuronidation and hydroxylation may be involved. However, these metabolic steps are saturable or less functional in cats. Moreover, the thioesterification of R(-) KTF in in vitro studies has been shown to be important in carnivores. The lack of correlation between clearance and chiral inversion process of R(-) KTF may be finally explained by deviation of thioesterification to other metabolic pathways of lipids and/or aminoacid conjugation, particulary glicine derivatives. 相似文献
4.
Carolina Parada Billy Ernst Sebastián Cornejo-Guzmán Francisco Santa Cruz Marco Sandoval Belmar Pablo Rivara Braulio Tapia Stéphane Gauthier Josefa Pino-Aguilera Ruben Escribano Carmen E. Morales 《水产资源保护:海洋与淡水生态系统》2021,31(2):253-272
- The Juan Fernandez Ridge, a vulnerable marine ecosystem located far off the coast of central Chile and formed by several seamounts, guyots and three islands (Robinson Crusoe, Santa Clara and Alejandro Selkirk), has recently been declared a Coastal Marine Protected Area of Multiple Uses with several National Parks embedded in it.
- Recent studies have highlighted the influence of remote and local oceanographic structures on the hydrographic dynamics of this ridge. However, there is still a gap in understanding how they affect the structure and dynamics of the surrounding insular planktonic communities.
- A hydroacoustic and oceanographic survey was conducted during the austral spring (October 2016), including hydrographic and zooplankton sampling around Robinson Crusoe Island. Oceanographic features were identified and tracked using satellite data (chlorophyll-a (Chl-a) and sea surface temperature) and modelling results.
- Two events of Chl-a increase relative to a threshold (>0.45 mg m−3) were forced by different physical processes, both affecting the western side of Robinson Crusoe Island. In event A during the cruise period, Chl-a subsurface maxima were associated with the arrival of a coastal meander originating on the continental shelf off Chile (remote process); the zooplankton was dominated by copepods and salps, with an evident coastal–oceanic gradient. In event B, Chl-a maxima were linked to a local upwelling forced by the intensification of a localized SSW wind. No influence of remote eddies or local Von Kármán vortices on Chl-a distribution was observed.
- These findings highlight the influence of remote and local physical processes on the structure of planktonic communities around Robinson Crusoe Island. Understanding the variability of these mechanisms and their effects at the base of the pelagic food web is critical in adopting an ecosystem-level approach.
5.
Pablo Aran Pablo Aguilar Thomas Bowen Matthew Farson Joseline Tapia Lara Vimercati John L. Darcy Adam J. Solon Dorota Porazinska Steven K. Schmidt Cristina Dorador 《水产资源保护:海洋与淡水生态系统》2021,31(8):2293-2299
- High elevation lakes are extreme ecosystems and serve as sentinels of various global changes.
- An expedition to Volcán Llullaillaco in 1996 discovered an unstudied high-elevation lake (6,170 m a.s.l.) that probably was formed as a result of the past eruptive events or climatic processes such as glacial retreat in the lake basin.
- This article describes an initial physical characterization of the lake and its microbial communities derived from two sampling expeditions in 2013 and 2016.
- The microbial community in the lake, with an area between 1.2 and 1.4 ha and a depth of 6.8 m, was dominated by Proteobacteria, Actinobacteria and Haloarchaea. In addition, 26 bacterial isolates were identified within the genera Subtercola, Xylophilus, Rhodanobacter, Mesorhizobium and Pseudomonas.
- Lago Llullaillaco is one of the highest recorded lakes in the world, and this study highlights the unique microbial diversity of this aquatic ecosystem and the importance of its preservation to understand the complex biological processes under polyextreme conditions.
6.
7.
N. Villarino S. Lesman A. Fielder D. García‐Tapia S. Cox M. Lucas J. Robinson S. A. Brown T. Martín‐Jiménez 《Journal of veterinary pharmacology and therapeutics》2013,36(4):329-339
The objective of the study was to assess the pharmacokinetics of tulathromycin in lung tissue homogenate (LT) and plasma from healthy and lipopolysaccharide (LPS)‐challenged pigs. Clinically healthy pigs were allocated to two dosing groups of 36 animals each (group 1 and 2). All animals were treated with tulathromycin (2.5 mg/kg). Animals in group 2 were also challenged intratracheally with LPS from Escherichia coli (LPS‐Ec) 3 h prior to tulathromycin administration. Blood and LT samples were collected from all animals during 17‐day post‐tulathromycin administration. For LT, one sample from the middle (ML) and caudal lobes (CL) was taken. The concentration of tulathromycin was significantly lower in the ML after the intratracheal administration of LPS‐E. coli (P < 0.02). In healthy pigs and LPS‐challenged animals, the distribution of the drug into the lungs was rapid and persisted at high levels for 17‐day postadministration. The distribution of the drug within the lung seems to be homogenous, at least between the middle and caudal lobes within dosing groups. The concentration versus time profile of the drug and pharmacokinetic parameters in two different lung areas (middle and caudal lobe) were consistent within the groups. The clinical significance of these findings is unknown. 相似文献
8.
9.
First identification and characterization of Tenacibaculum dicentrarchi isolated from Chilean red conger eel (Genypterus chilensis,Guichenot 1848)
下载免费PDF全文
![点击此处可从《Journal of fish diseases》网站下载免费的PDF全文](/ch/ext_images/free.gif)
10.
Rubn Alonso Contreras‐Tapia María Elena Castellanos‐Pez S. Nandini Marcela Ivonne Benítez‐Díaz‐Mirn Gabriela Garza‐Mourio 《Aquaculture Research》2020,51(5):2102-2111
The effect of a commercial probiotic (NanoCrusta, Altacrusta, Mexico City, Mexico) on the growth performance of Brachionus calyciflorus Pallas, 1766, was evaluated. In a first approach, probiotics were supplied in four densities (2.0 × 103, 1.1 × 105, 2.1 × 105 and 2.1 × 106 cells/ml), alone and in combination with Chlorella vulgaris (1 × 106 cells/ml). The test rotifer did not grow on the probiotic alone. However, when probiotics + C. vulgaris were added, the maximum densities (Dmax; ind/ml) and population growth rates (r) observed were higher. In the second experiment, probiotics were supplied at five higher densities (2.1 × 106, 4.2 × 106, 8.5 × 106, 1.7 × 107 and 3.4 × 107 cells/ml) with C. vulgaris and a control treatment with only C. vulgaris (probiotic‐free). Treatments supplied with probiotics between 2.1 × 106 and 1.7 × 107 cells/ml showed significantly higher Dmax and r than the control treatment. The results showed a positive effect of probiotic bacteria when supplied with C. vulgaris. The best outcome showed a Dmax 2.16 times and an r 1.63 times higher than the density of the control treatment. Growth rates were higher in the treatments with probiotics compared to the control. We conclude that application of NanoCrusta is feasible to improve B. calyciflorus production, but the effects need to be tested in larger scales. 相似文献