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Takada-Iwao A Uto T Mukai T Okada M Futo S Shibata I 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):581-586
To facilitate the control of progressive atrophic rhinitis (PAR) of swine caused by toxigenic Pasteurella multocida, an enzyme-linked immunosorbent assay (ELISA) and a serum neutralization test (NT) have recently been developed to detect antibodies against the P. multocida dermonecrotic toxin (PmDNT). However, the NT is a cumbersome and time-consuming technique. To overcome these drawbacks, we developed an indirect ELISA, using recombinant PmDNT expressed in Escherichia coli, for the detection of antibodies to PmDNT in serum samples from pigs. The practical usefulness of this ELISA was compared with the NT using serum samples obtained from experimentally infected and naturally infected pigs. In the pigs experimentally inoculated with vaccine including PmDNT toxoid, the ELISA and neutralization antibodies were detected at almost the same time, and a good correlation was demonstrated between both tests (P<0.01, R(2)=0.807). Therefore, the ELISA can be used to evaluate the immune reaction of pigs after vaccination with P. multocida toxoid. In a survey conducted on a field herd with a history of clinical AR, the seropositivity by ELISA in pigs of age 4.5-6 months was increased even though the NT was negative, and the correlation was low between the results obtained with the two tests (P<0.01, R(2)=0.38). Therefore, the results indicated that this ELISA might be a useful alternative to the NT currently used to detect the antibody to PmDNT after vaccination or infection with P. multocida. 相似文献
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(pp. 17–24)
A trial calculation was performed of the environmental nitrogen-assimilation capacity and the amount of nitrogen input based on various statistical data, which were compiled from each city, town and village in Hokkaido prefecture. The relationship between the excess quantity of nitrogen, after nitrogen input, and the environmental nitrogen-assimilation capacity and the nitrate-nitrogen concentration of the groundwater was considered.
Environmental nitrogen-assimilation capacity = nitrogen output by the crops + acceptable level of residual nitrate in the soil profile.*
* It is calculated by the amount of nitrate precipitation evapotranspiration ×10 mg L−1 .
A trial calculation was performed of the environmental nitrogen-assimilation capacity and the amount of nitrogen input based on various statistical data, which were compiled from each city, town and village in Hokkaido prefecture. The relationship between the excess quantity of nitrogen, after nitrogen input, and the environmental nitrogen-assimilation capacity and the nitrate-nitrogen concentration of the groundwater was considered.
Environmental nitrogen-assimilation capacity = nitrogen output by the crops + acceptable level of residual nitrate in the soil profile.*
- 1)
The average value of the environmental nitrogen-assimilation capacity in Hokkaido Prefecture was observed to be 183 kg ha
−1 . The maximum and minimum values of the environmental nitrogen-assimilation capacity were 308 kg ha−1 and 94 kg ha−1 , respectively. When the average value of the environmental nitrogen-assimilation capacity with respect to main agricultural land use was compared across municipalities, it was largely in the following order · grassland (218 kg ha−1 ), upland (169 kg ha−1 ), and paddy land (157 kg ha−1 ).
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Yuji Sumii Kentaro Kamiya Takehiko Nakamura Kenta Tanaka Takumi Kaji Junya Mukomura Naoyuki Kotoku Masayoshi Arai 《Marine drugs》2022,20(2)
The current tuberculosis treatment regimen is long and complex, and its failure leads to relapse and emergence of drug resistance. One of the major reasons underlying the extended chemotherapeutic regimen is the ability of Mycobacterium tuberculosis to attain a dormant state. Therefore, the identification of new lead compounds with chemical structures different from those of conventional anti-tuberculosis drugs is essential. The compound 3-(phenethylamino)demethyl(oxy)aaptamine (PDOA, 1), isolated from marine sponge of Aaptos sp., is known as an anti-dormant mycobacterial substance, and has been reported to be effective against the drug resistant strains of M. tuberculosis. However, its target protein still remains unclear. This study aims to clarify the structure–activity relationship of 1 using 15 synthetic analogues, in order to prepare a probe molecule for detecting the target protein of 1. We succeeded in creating the compound 15 with a photoaffinity group that retained antimicrobial activity, which proved to be a suitable probe molecule for identifying the target protein of 1. 相似文献
4.
Takehiko Shimada Hiroko Hayama Kouichi Nishimura Masami Yamaguchi Masao Yoshida 《Euphytica》2001,117(1):85-90
RAPD analysis was applied to reveal the genetic diversities of 4 speciesof subg. Lithocerasus within the genus Prunus using 40accessions representing the subgenera Prunophora, Amygdalus,Lithocerasus and Cerasus. The accession of subg. Lithocerasus are phenotypically similar to members of subgenus Cerasus but different with them in interspecific crossing tests and isozymeprofiles. Two major clusters, `Prunophora and Amygdalus group' and `Cerasus group' were constructed in the phenogram. We revealthat the examined 4 species of subg. Lithocerasus species; P.tomentosa Thunb., P. japonica Thunb., P. glandulosa Thunb.and P. besseyi Bailey, were genetically closer to the members ofsubgenera Prunophora and Amygdalus than to subg. Cerasus. 相似文献
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Akira Otsuki Takehiko Omi Shinya Hashimoto Morihiro Aizaki Noriko Takamura 《Water, air, and soil pollution》1994,76(3-4):383-396
A method for HPLC determination of phycocyanin in phytoplankton samples using gelchromatography with a fluorescence detector was developed to examine the use of phycocyanin as an index of the appearance and progress of cyanobacterial blooms in highly eutrophic lake. At least two types of phycocyanin with different molecular weights, each spectroscopically different from phycoerythrin and allophycocyanin, were found in natural phytoplankton samples. Changes in phycocyanin concentrations were clearly coupled to changes in chlorophyll-a concentrations during June to October while cyanobacterial blooms were occurring. The chlorophyll-a to phycocyanin relationship was linear at chlorophyll-a concentrations of less than 250 μg L?1. The relationship between cyanobacterial carbon and phycocyanin concentration was also linear, suggesting that phycocyanin content may be a useful index of cyanobacterial biomass in highly eutrophic lakes where large cyanobacterial blooms occur. 相似文献
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The cell-associated adsorption of thorium or uranium from the solution containing each metal only at pH 3.50 using various microorganisms was examined. Among the species tested, high thorium adsorption abilities were exhibited by strains of the gram-positive bacteria Arthrobacter nicotianae IAM12342, Bacillus megaterium IAM1166, B. Subtilis IAM1026, Micrococcus luteus IAM1056, Rhodococcus erythropolis IAM1399, and Streptomyces levoris HUT6156. And high uranium adsorption abilities were found in some gram-positive bacterial strains S. albus HUT6047, S. levoris HUT6156, and A. nicotianae IAM12342. Among these highly efficient thorium and uranium adsorbing microorganisms, S. levoris, which could adsorb the largest amount of uranium from the aqueous solution at pH 3.50, could adsorb about 383 mol thorium and 390 mol uranium per gram dry weight of microbial cells from the solution containing thorium or uranium at pH 3.50. The amount and time course of thorium adsorbed were almost unaffected by co-existing uranium; however, the adsorption of thorium was faster when carried out after the adsorption of uranium. Thorium adsorption also became faster when uranium was added after thorium adsorption. The effect of pH on thorium adsorption was also discussed. 相似文献
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Nakanishi Kosuke Usio Nisikawa Yokomizo Hiroyuki Takashima Tadao Hayashi Takehiko I. 《Paddy and Water Environment》2022,20(1):177-183
Paddy and Water Environment - Rice paddy fields are important habitat for many dragonfly species. In Japan, populations of dragonflies inhabiting rice paddies, in particular Sympetrum (Odonata:... 相似文献
10.
Akito Kaga Takehiko Shimizu Satoshi Watanabe Yasutaka Tsubokura Yuichi Katayose Kyuya Harada Duncan A. Vaughan Norihiko Tomooka 《Breeding Science》2012,61(5):566-592
Genetic variation and population structure among 1603 soybean accessions, consisted of 832 Japanese landraces, 109 old and 57 recent Japanese varieties, 341 landrace from 16 Asian countries and 264 wild soybean accessions, were characterized using 191 SNP markers. Although gene diversity of Japanese soybean germplasm was slight lower than that of exotic soybean germplasm, population differentiation and clustering analyses indicated clear genetic differentiation among Japanese cultivated soybeans, exotic cultivated soybeans and wild soybeans. Nine hundred ninety eight Japanese accessions were separated to a certain extent into groups corresponding to their agro-morphologic characteristics such as photosensitivity and seed characteristics rather than their geographical origin. Based on the assessment of the SNP markers and several agro-morphologic traits, accessions that retain gene diversity of the whole collection were selected to develop several soybean sets of different sizes using an heuristic approach; a minimum of 12 accessions can represent the observed gene diversity; a mini-core collection of 96 accession can represent a major proportion of both geographic origin and agro-morphologic trait variation. These selected sets of germplasm will provide an effective platform for enhancing soybean diversity studies and assist in finding novel traits for crop improvement. 相似文献