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1.
Lancefield group C Streptococcus dysgalactiae (GCSD) causes severe necrotic lesions in the caudal peduncle in the genus Seriola farmed in Japan. To develop a sero‐diagnostic method for GCSD infection in farmed fish, we attempted to identify a surface immunogenic protein that induces an antibody after infection with GCSD by immunoblot analysis using sera collected from infected fish. A protein obtained from sodium dodecyl sulfate (SDS) extracts of GCSD was identified as S. dysgalactiae surface immunogenic protein (Sd‐Sip). Sd‐Sip exhibited more than 94% homology with a surface antigen or a hypothetical protein from S. dysgalactiae mammalian isolates at the nucleotide sequence level. Expression of the recombinant Sd‐Sip (rSd‐Sip) was confirmed by immunoblot analysis, that is, its reactivity to GCSD‐infected sera. Antibody detection ELISA using rSd‐Sip and their usefulness for diagnosis of GCSD infection were examined. GCSD‐infected sera collected from farmed amberjack, Seriola dumerili (Risso), showed strong reaction with immobilized rSd‐Sip. Meanwhile, sera immunized by other pathogenic bacteria of fish were showed ELISA values similar to those of non‐infected sera. These results of this study suggest that the antibody detection ELISA using rSd‐Sip is an effective diagnostic method for GCSD infection in fish. 相似文献
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Effects of feeding and animal performance on nitrogen, phosphorus and potassium excretion by Holstein cows 总被引:1,自引:0,他引:1
Hideki KOJIMA Shin-ichi KUME Kazuhisa NONAKA Tomoko OSHITA Takaharu KOZAKAI Hiroyuki HIROOKA 《Animal Science Journal》2005,76(2):139-145
Thirty‐four Holstein dry cows and 16 lactating cows were used in balance trials to identify the effects of feeding and animal performance on nitrogen (N), phosphorus (P) and potassium (K) excretion by dairy cows, and to develop prediction models for these excreta. Orchard grass silage, corn silage, alfalfa silage or timothy hay were offered to dry cows. Orchard grass silage or alfalfa silage, and concentrates were offered to lactating cows. In the statistical analysis, the independent variables were bodyweight (kg), dry matter (DM) intake (kg/day), milk yield (for lactating cows only, kg/day), water intake (free water plus water in feed, kg/day), intake (g/day) of N, P and K and dietary contents (% of DM) of crude protein, P and K. The dependent variables were N, P and K excretion (g/day) in feces and urine. In both dry and lactating cows, intake of N, P and K had large effects on corresponding excretion. The results indicated that a decrease in the intake of N, P and K could decrease the corresponding excretion. Further research by path analysis showed that K intake positively affected urinary N excretion in dry cows indirectly, through water intake and urine volume. 相似文献
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Jun TAMURA Takaharu ITAMI Tomohito ISHIZUKA Sho FUKUI Kenjirou MIYOSHI Tadashi SANO Kazuto YAMASHITA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):865-869
To determine the reference level of central venous oxygen saturation (ScvO2)
and clinical efficacy of central venous blood gas analysis, partial pressures of oxygen
and carbon dioxide, pH, oxygen saturation, base excess (B.E.) and HCO3
concentration were compared between simultaneously obtained central venous and arterial
blood samples from conscious healthy 6 dogs and 5 cats. Comparisons between arteriovenous
samples were performed by a paired t-test and Bland-Altman analysis.
Between arteriovenous samples, B.E. showed good agreement, but there were significant
differences in other parameters in the dogs, and no good agreement was detected in cats.
The ScvO2 in dogs and cats were 82.3 ± 3.5 and 62.4 ± 13.5%, respectively.
Central venous blood gas analysis is indispensable, especially in cats. 相似文献
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Use of Jeffries acid-oxalate treatment in particle-size analyses of Ando soils for breaking up the sand- and silt-size aggregates bound by allophane, allophane-like constituents and hydrous iron oxides was studied. The analyses by this method and those by a method using sonic-wave oscillation were compared for nine Ando soil samples having different clay-mineral compositions and organic-matter contents. There were minor differences in the sand contents between the two analyses, but the clay contents were higher and the silt contents were lower after Jeffries acid oxalate treatment.
Characterization of the silt-size separates by an X-ray powder method, water vapor adsorption and electron microscopy indicated that the lower silt contents after Jeffries acid oxalate treatment were mainly due to dissolution of allophane and allophane-like constituents, whereas the silt-size separates after sonic-wave treatment still contained these mineral constituents. The proposed procedure for particle-size analysis was also useful for two ferruginous soils representing the Hydrandept and Torrox. 相似文献
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Haruka TAMOGI Takaharu ITAMI Ai HORI Norihiko OYAMA Tadashi SANO Kazuto YAMASHITA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2020,82(11):1714
An 11-year-old Toy Poodle underwent a computed tomography examination with contrast (iohexol) enhancement under anesthesia. Heart rate and R-wave amplitude on electrocardiogram (ECG) increased 2.5 min after iohexol administration, and end-tidal carbon dioxide decreased to 12 mmHg. A progressive ST segment depression was observed on ECG. Subsequently, the ECG waveform changed to ventricular fibrillation. However, spontaneous circulation returned following cardiopulmonary resuscitation. Myocardial ischemia or anaphylactic shock was suspected in the dog, which explains the ST segment depression observed on ECG. When performing radiological examinations with a contrast agent, the ECG waveform changes, such as an increase in heart rate, R-wave amplitude, or ST segment depression, should be carefully monitored. This might enable early detection of cardiac dysfunction and the ensuing cardiac arrest in dogs. 相似文献
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Issei Nishiki Mikiko Furukawa Sho Matui Toshiaki Itami Toshihiro Nakai Terutoyo Yoshida 《Fisheries Science》2011,77(3):367-373
In Japan, Lactococcus garvieae infection has been the main fish disease in aquaculture. Although commercial oral and injectable vaccines have been used
to prevent L. garvieae infection in Japan, L. garvieae has been isolated not only from unvaccinated fish but also from vaccinated fish in which immunity induced by vaccination
had diminished. In order to obtain epidemiological information on this fish pathogen, we conducted biased sinusoidal field
gel electrophoresis (BSFGE) pattern analysis and phage typing of L. garvieae isolates (n = 427) from fish in Japan. These isolates were obtained from 13 different fish species between 1980 and 2007. In the BSFGE
analysis, L. garvieae isolates were classified into 17 groups (S1–S17) based on the SmaI digestion patterns and into four groups (A1–A4) based on the ApaI digestion patterns. Phage typing revealed five different phage susceptibility profiles (A–E) in L. garvieae isolates. Since 2005, comparisons of the results of phage typing and BSFGE have indicated the presence of a novel genotype
(S16/A4) with phage type E. All the strains belonging to this type showed lincomycin sensitivity. 相似文献
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Jean Fall Gunimala Chakraborty Tomoya Kono Minoru Maeda Yoshihiro Suzuki Toshiaki Itami Masahiro Sakai 《Fisheries Science》2011,77(1):129-134
Vibrio nigripulchritudo is considered one of the major pathogens threatening shrimp aquaculture. In this study, we developed a novel and highly specific
quantitative loop-mediated isothermal amplification (Q-LAMP) assay. A set of four specific primers were designed targeting
the V. nigripulchritudo intergenic spacer region. The reaction time and temperature were optimized for 60 min at 63°C. Quantitative analysis was
then performed by measuring the turbidity of the reaction solution using a real-time turbidimeter, allowing for quantification
of the initial DNA concentration with a sensitivity of 102 copy numbers equivalent to 2.3 colony forming units/ml or 0.3 fg/μl. The LAMP assay was able to specifically detect two representative
strains of V. nigripulchritudo, whereas other Vibrio and non-Vibrio species were not amplified. A standard curve was generated for V. nigripulchritudo by plotting the threshold time (T
t) versus the log of bacterial number. A high correlation coefficient (R
2 = 0.9749) was observed for the Q-LAMP reaction. In conclusion, Q-LAMP assay is a sensitive, rapid, and simple tool that can
be used for the detection and quantification of V. nigripulchritudo in shrimp, thereby facilitating surveillance of vibriosis infection. 相似文献