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1.
The inactivation of pollen by soft X-ray irradiation and subsequent artificial pollination are time-intensive practices used in the production of seedless watermelons (Citrullus lanatus L.). Watermelon generally has a lot of staminate flowers; however, they only have a small amount of pollen. Watermelon pollen cannot be used in pollination under the present situation. Therefore, the objective of this study was to determine which is the most effective pollen extender medium for cultivation of watermelon with soft X-ray irradiated pollen. In this experiment, ‘Agar,’ ‘Marriage powder,’ and ‘Sekishoshi’ (Lycopodium dyed red with safflower pigment) were used as extender media with soft X-ray irradiated pollen at equal or twice the weight of the pollen. When ‘Sekishoshi’ was used as an extender medium, fruit set was very low. A lot of deformed fruit was produced when agar was used with the pollen. On the other hand, when ‘Marriage powder’ was used in equal proportions with the pollen, fruit set was about 70% and, moreover, Brix was high. Thus, the mixture of ‘Marriage powder’ with an equal amount of pollen was the best for use in actual cultivation. We conclude, then, that soft X-ray irradiated pollen in an extender medium can be effectively adapted for producing seedless watermelons. 相似文献
2.
The impact of nitrogen(N)deposition on dissolved organic carbon(DOC)fractions in throughfall is not well understood.We performed a laboratory experiment and compared DOC leaching from canopy leaves after dipping leaves in pure water(control)and NH4NO3 solution(N-treatment)for 18 h.Net changes of DOC,NH4^+,NO3^-,SO4^2-,K^+,Mg^2+,Ca^2+and H^+contents after dipping leaves were determined by comparing solutions with and without leaves.We recorded no differences of DOC leaching between control and N-treatment,implying that N deposition had minor impacts on canopy DOC production.This confirmed that previous experiments testing the effects of N addition on DOC dynamics without considering the effects of the canopy reaction successfully described the real situation.We also confirmed the previously-reported canopy exchange process in spite of a high background N deposition at our study site.N-treatment significantly increased base cation leaching,especially K^+,and the increase was positively correlated with foliar NH4?retention.Net leaching of H^+and SO4^2-was not affected by the N-treatment. 相似文献
3.
Lactobacillus rhamnosus GG SpaC pilin subunit binds to the carbohydrate moieties of intestinal glycoconjugates 下载免费PDF全文
Keita Nishiyama Shintaro Ueno Makoto Sugiyama Yuji Yamamoto Takao Mukai 《Animal Science Journal》2016,87(6):809-815
Lactobacillus rhamnosus GG (LGG) is a well‐established probiotic strain. The beneficial properties of this strain are partially dependent on its prolonged residence in the gastrointestinal tract, and are likely influenced by its adhesion to the intestinal mucosa. The pilin SpaC subunit, located within the Spa pili structure, is the most well studied LGG adhesion factor. However, the binding epitopes of SpaC remain largely unknown. The aim of this study was to evaluate the binding properties of SpaC to the carbohydrate moieties of intestinal glycoconjugates using a recombinant SpaC protein. In a competitive enzyme‐linked immunosorbent assay, SpaC binding was markedly reduced by addition of purified mucin and the mucin oligosaccharide fraction. Histochemical staining revealed that the binding of SpaC was drastically reduced by periodic acid treatment. Moreover, in the surface plasmon resonance‐based Biacore assay, SpaC bound strongly to the carbohydrate moieties containing β‐galactoside at the non‐reducing terminus of glycolipids. We here provide the first demonstration that SpaC binds to the oligosaccharide chains of mucins, and that the carbohydrate moieties containing β‐galactoside at the non‐reducing termini of glycoconjugates play a crucial role in this binding. Our results demonstrate the importance of carbohydrates of SpaC for mucus interactions. 相似文献
4.
Satoshi Furukawa Naho Tsuji Seigo Hayashi Yusuke Kuroda Masayuki Kimura Chisato Hayakawa Kazuya Takeuchi Akihiko Sugiyama 《Journal of toxicologic pathology》2022,35(1):37
We investigated the morphological effects of testosterone on placental development in a rat model of polycystic ovarian syndrome (PCOS). Testosterone propionate (TP), which was subcutaneously administered to pregnant rats with 5 mg/animal from gestation day (GD) 14 to GD 18, induced a maternal weight reduction without mortality or clinical signs from GD 19 onwards. A decrease in fetal and placental weight, an increase in intrauterine growth retardation (IUGR) rates, and histological changes in the placenta were observed on GD 21 but not on GD15 or 17. Histopathologically, on GD 21, the trophoblast septa thickened, and the maternal sinusoids were narrowed in the labyrinth zone, resulting in a small placenta. Additionally, the placental weight, thickness, and histological morphology in the labyrinth zone on GD 21 in the TP-treated group were nearly identical to those on GD 17 in the control and TP-treated groups. Therefore, it was assumed that the testosterone-induced small placenta was induced in association with the developmental inhibition of the fetal part of the placentas from GD 17 onwards. 相似文献
5.
Molecular Characterization and Diagnosis of QoI Resistance in Cucumber and Eggplant Fungal Pathogens
Ishii H Yano K Date H Furuta A Sagehashi Y Yamaguchi T Sugiyama T Nishimura K Hasama W 《Phytopathology》2007,97(11):1458-1466
ABSTRACT The molecular mechanism of QoI fungicide resistance was studied using isolates of cucumber Corynespora leaf spot fungus (Corynespora cassiicola) and the eggplant leaf mold (Mycovellosiella nattrassii). In both pathogens, a mutation at position 143 from glycine to alanine (G143A) was detected in the cytochrome b gene that encodes for the fungicide-targeted protein. Moreover, the nucleotide sequence at amino acid position 143 was converted from GGT or GGA in sensitive (wild-type) to GCT or GCA in resistant (mutant-type) isolates. The methods of polymerase chain reaction restriction fragment length polymorphism commonly used for QoI resistance monitoring were employed successfully, leading to the amplified gene fragment from resistant isolates being cut with the restriction enzyme ItaI. However, heteroplasmy (the coexistence of wild-type and mutated alleles) was found when the resistant isolates of C. cassiicola, M. nattrassii, and Colletotrichum gloeosporioides (strawberry anthracnose fungus) were subcultured in the presence or absence of QoI fungicides. QoI resistance of cucumber powdery and downy mildew isolates persisted for a few years following the removal of the selection pressure imposed by the fungicide under both laboratory and commercial greenhouse conditions. The proportion of mutated sequences in cytochrome b gene decreased over time in the pathogen population. The protective efficacy of the full dose of azoxystrobin decreased when the populations of powdery and downy mildews contained resistant isolates at 10%. Using FMBIO, a fluorescence bio-imaging analyzer, the mutant allele from the QoI-resistant isolates could be detected at the level of 1%, whereas the detection sensitivity of ethidium-bromide-stained gels was approximately 10 times lower. 相似文献
6.
7.
Isothermal amplification of rabies virus gene 总被引:1,自引:0,他引:1
8.
J Sugiyama 《Journal of agricultural and food chemistry》1999,47(7):2715-2718
The relationship between the sugar content and absorption spectra was investigated using a near-infrared (NIR) spectrometer to visualize the sugar content of a melon. The absorbance at 676 nm, which is close to the absorption band of chlorophyll, exhibited a strong inverse correlation with the sugar content. A high-resolution cooled charged couple device imaging camera fitted with a band-pass filter of 676 nm was used to capture the spectral absorption image. The calibration method used for converting the absorbance values on the image into the degrees Brix sugar content was developed in accordance with NIR spectroscopy techniques. When this method was applied to each pixel of the absorption image, a color distribution map of the sugar content was constructed. 相似文献
9.
To study the effect of pollination on fruit development in gynoecious and parthenocarpic cucumbers (Cucumis sativus L. cv. NK × AN8), the number of cells, cell size, mitotic index, histone H4 gene expression, and concentrations of endogenous cytokinins and auxin were compared in fruits that had developed from pollinated and non-pollinated flowers. Fresh weight was more in the pollinated group 4–12 days after anthesis (DAA) in both winter and spring, and 2–6 DAA in the summer. In winter, mitotic index increased from anthesis to 2 DAA and then decreased gradually in the pollinated group but immediately after anthesis in the non-pollinated group. Histone H4 gene in the pericarp zone was expressed more strongly during the period from pre-anthesis to 2 DAA in the pollinated group in summer. Concentrations of zeatin, isopentenyladenine, and indole-3-acetic acid (IAA) were higher in the non-pollinated group 2 and 4 DAA and peaked 4 DAA in spring whereas isopentenyladenine and IAA showed no distinct peaks in the pollinated group. These results are incompatible with the idea that pollination activates cell division by stimulating the synthesis of cytokinins and auxin in cucumber fruits. 相似文献
10.