Cystadenocarcinoma of the intrahepatic bile duct in a dog

A 14-year-old spayed female Shih-Tzu was referred to the Veterinary Medical Teaching Hospital of Konkuk University for evaluation of an abdominal mass. In diagnostic imaging, two large cystic masses were identified. The affected liver lobes were surgically resected, and the specimens were submitted for histopathological evaluation and immunohistochemical staining. The two cystic lesions were diagnosed as biliary cystadenocarcinoma (BCAC). Recurrence and regional invasion were identified on ultrasonography 36 days postoperatively. The patient died on postoperative day 271. To the best of our knowledge, previously reported case studies of BCAC in dogs presented limited clinical information. In this report, we present a detailed picture comprising a range of clinical information and histopathological examination of BCAC in a dog.     

Ultrasonographic evaluation of tracheal collapse in dogs

Tracheal ultrasonography was performed to measure the width of the tracheal ring shadow and to assess the clinical relevance of these measurements for identifying tracheal collapse. The first tracheal ring width (FTRW) and thoracic inlet tracheal ring width (TITRW) were measured on both expiration and inspiration. The mean of the FTRW width (129 dogs) was greater in expiration (10.97 ± 1.02 mm, p = 0.001) than that in inspiration (9.86 ± 1.03 mm). For 51 normal dogs, the mean of the TITRW width was greater in expiration (9.05 ± 1.52 mm, p = 0.001) than in inspiration (8.02 ± 1.43 mm). For 78 tracheal collapse dogs, the mean of the TITRW width was greater in expiration (15.89 ± 1.01 mm, p = 0.001) than in inspiration (14.85 ± 1.17 mm). The TITRW/FTRW ratio of the normal dogs was higher (p = 0.001) in expiration (0.81 ± 0.09) than that in inspiration (0.79 ± 0.10). When compared between the normal and tracheal collapse dogs, the TITRW/FTRW ratio was also increased (p = 0.001) both in expiration (1.54 ± 0.09) and inspiration (1.47 ± 0.08), respectively. Based on these results, the cutoff level of the TITRW/FTRW ratio was statistically analyzed according to the receiver operating characteristic curve and it could be set at 1.16 in expiration and at 1.13 in inspiration. We have demonstrated that tracheal ultrasonography is a useful technique for the evaluation of tracheal collapse and it can be a supportive tool together with the radiographic findings for making the correct diagnosis.     

长白山区野生地椒百里香嫩枝扦插繁殖研究

以长白山区野生地椒百里香为试材,研究了不同浓度、不同生长调节剂、不同时间处理对地椒百里香嫩枝扦插繁殖的影响。结果表明:植物生长调节剂对平均生根率的影响为ABTNAAIBA,分别为94.22%、90.69%、81.33%。但是嫩枝扦插后根部长势最好在NAA1 000mg/L(1s)。因此,长白山区野生地椒百里香嫩枝扦插时,建议选择ABT生根剂。选用ABT生根剂时,处理浓度间差异不大,但浓度500mg/L(2min)时优于其它处理组。当选用NAA溶液时,处理浓度间差异较大,且随着处理浓度的增加效果更佳,最佳处理为1 000mg/L(1s)。当选用IBA溶液时,随着浓度增加,生根率及根部长势越差,50mg/L(12h)为最佳处理。     

Substitution effect of Undaria pinnatifida with citrus (Citrus unshiu,Marcovitch) peel by‐product in feed on the growth,body composition and air exposure stressor of juvenile abalone (Haliotis discus,Reeve 1846)

Substitution effect of Undaria pinnatifida with citrus peel by‐product (CPB) on growth, body composition and air exposure stressor of abalone was determined. A total of 1,080 abalone were distributed into 18 net cages. Five formulated diets were prepared in triplicate. The CPB0 diet contained 200 g/kg U. pinnatifida. The 250, 500, 750 and 1,000 g/kg U. pinnatifida were substituted with the equal amount of CPB, referred to as the CPB250, CPB500, CPB750 and CPB1000 diets, respectively. Finally, dry U. pinnatifida was prepared. Abalone were fed for 16 weeks and then subjected to air exposure stressor for 24 hr. The cumulative mortality of abalone was monitored for the following 4 days after 24‐hr air exposure. Survival, weight gain and specific growth rate (SGR) of abalone fed all formulated diets were greater than those of abalone fed the U. pinnatifida. The greatest weight gain and SGR were achieved in abalone fed the CPB500 diet. The chemical composition of the soft body of abalone was not affected by the experimental diets. Higher cumulative mortality was observed in abalone fed the CPB0 and dry U. pinnatifida at 16 hr after 24‐hr air exposure compared to abalone fed all other diets. In conclusion, U. pinnatifida could be completely substituted with CPB in abalone feed.     

茅苍术提取物及其活性成分的杀蚊虫作用

用乙醇超声提取法提取茅苍术根茎物质,并进行杀蚊有效活性成分分离,采用幼虫浸渍法测定其对敏感型淡色库蚊、野生型地下家蚊和白纹伊蚊3龄幼虫的活性。结果发现:乙醇粗提物对淡色库蚊、地下家蚊和白纹伊蚊的24h LC_(50)值分别为22.71、36.30、23.51μg/mL。不同溶剂萃取物对白纹伊蚊生物活性测定结果表明,活性成分主要在正己烷相中,其LC_(50)为16.87μg/mL;而乙酸乙酯相和水相成分基本无活性。进一步对正己烷萃取物以硅胶柱层析分离的不同成分进行生物活性测定,活性成分主要为H2和H6;白纹伊蚊3龄幼虫在其25μg/mL质量浓度下24h死亡率均达100%。结果表明茅苍术的杀虫活性成分对敏感型淡色库蚊、野生型地下家蚊和白纹伊蚊的幼虫均具有较强生物活性,具有新型杀蚊剂开发潜力。