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Amaresh Kumar NAYAK Rahul TRIPATHI Manish DEBNATH Chinmaya Kumar SWAIN Biswaranjan DHAL Shanmugam VIJAYKUMAR Anshuman Debasis NAYAK Sangita MOHANTY Mohammad SHAHID Anjani KUMAR Manoj RAJAK Khitish Chandra MOHARANA Dibyendu CHATTERJEE Susmita MUNDA Pravat GURU Rubina KHANAM Banwari LAL Priyanka GAUTAM Suschismita PATTANAIK Arvind Kumar SHUKLA Nuala FITTON Pete SMITH Himanshu PATHAK 《土壤圈》2023,33(3):448-462
Scarcity of water and emission of greenhouse gases (GHGs) are the two key environmental issues affecting crop production in India.Reducing the carbon footprint (CF) and water footprint (WF) of crop production can help to mitigate the environmental hazards that stem from GHG emissions and water scarcity.The CFs and WFs of three major cereal crops,rice,wheat,and maize,were estimated for the year 2014 under the environmental conditions in India,based on national statistics and other data sources.To... 相似文献
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Diet analysis of small mammal pests: A comparison of molecular and microhistological methods
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Knowledge of what pest species are eating is important to determine their impact on stored food products and to plan management strategies accordingly. In this study, we investigated the food habits of 2 rodents, Rattus rattus (ship rat) and Mus musculus castaneus (house mouse) as well as an insectivore, Suncus murinus (shrew), present in human dwellings. Both a microhistological approach and a DNA barcoding approach were used in the present study. Following DNA extraction, amplification was performed using group‐specific primers targeting birds, plants and invertebrates. Resulting polymerase chain reaction products were sequenced and analyzed to identify the different prey species present in the gut contents. The findings from the application of both techniques were in agreement, but the detection of prey type with each technique was different. The DNA barcoding approach gave greater species‐level identification when compared to the microhistological method, especially for the invertebrate and avian prey. Overall, with both techniques, 23 prey taxa were identified in the gut contents of the 3 species, including 15 plants, 7 insects and a single bird species. We conclude that with a selection of suitable “barcode genes” and optimization of polymerase chain reaction protocols, DNA barcoding can provide more accurate and faster results. Prey detection from either technique alone can bias the dietary information. Hence, combining prey information of both microhistological analysis and DNA barcoding is recommended to study pest diet, especially if the pest is an omnivore or insectivore species. 相似文献
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