Monoamine oxidase activity in some brain areas of sheep after hormonal stimulation

The influence of hormonal superovulatory preparations Folistiman (450 IU FSH, Spofa, Prague) and serum gonadotropin (1500 IU PMSG, Spofa, Prague) on monoamine oxidase (MAO), the degradative enzyme of catecholamines, was investigated in some areas of the brain regulating reproductive functions (area preoptica of the hypothalamus, pituitary gland, and pineal gland) in ewes with synchronized oestrus (20 mg chlorsuperlutin) during the oestrous period using a radiochemical method. After intramuscular administration of 1500 IU PMSG, marked increase of MAO activity was found in the area preoptica (p < 0.05) and in the pituitary gland (p < 0.01) in comparison with the control group. No change occurred in MAO activity after ovarian stimulation with FSH. Administration of the above superovulatory preparations failed to induce MAO activity in the pineal gland of sheep.     

Leaf respiration at different canopy positions in sweetgum (Liquidambar styraciflua) grown in ambient and elevated concentrations of carbon dioxide in the field

Trees exposed to elevated CO2 partial pressure ([CO2]) generally show increased rates of photosynthesis and growth, but effects on leaf respiration are more variable. The causes of this variable response are unresolved. We grew 12-year-old sweetgum trees (Liquidambar styraciflua L.) in a Free-Air CO2 Enrichment (FACE) facility in ambient [CO2] (37/44 Pa daytime/nighttime) and elevated [CO2] (57/65 Pa daytime/nighttime) in native soil at Oak Ridge National Environmental Research Park. Nighttime respiration (R(N)) was measured on leaves in the upper and lower canopy in the second (1999) and third (2000) growing seasons of CO2 fumigation. Leaf respiration in the light (R(L)) was estimated by the technique of Brooks and Farquhar (1985) in the upper canopy during the third growing season. There were no significant short-term effects of elevated [CO2] on R(N) or long-term effects on R(N) or R(L), when expressed on an area, mass or nitrogen (N) basis. Upper-canopy leaves had 54% higher R(N) (area basis) than lower-canopy leaves, but this relationship was unaffected by CO2 growth treatment. In August 2000, R(L) was about 40% of R(N) in the upper canopy. Elevated [CO(2)] significantly increased the number of leaf mitochondria (62%), leaf mass per unit area (LMA; 9%), and leaf starch (31%) compared with leaves in ambient [CO(2)]. Upper-canopy leaves had a significantly higher number of mitochondria (73%), N (53%), LMA (38%), sugar (117%) and starch (23%) than lower-canopy leaves. Growth in elevated [CO2] did not affect the relationships (i.e., intercept and slope) between R(N) and the measured leaf characteristics. Although no factor explained more than 45% of the variation in R(N), leaf N and LMA were the best predictors for R(N). Therefore, the response of RN to CO2 treatment and canopy position was largely dependent on the magnitude of the effect of elevated [CO2] or canopy position on these characteristics. Because elevated [CO2] had little or no effect on N or LMA, there was no effect on R(N). Canopy position had large effects on these leaf characteristics, however, such that upper-canopy leaves exhibited higher R(N) than lower-canopy leaves. We conclude that elevated [CO2] does not directly impact leaf respiration in sweetgum and that barring changes in leaf nitrogen or leaf chemical composition, long-term effects of elevated [CO2] on respiration in this species will be minimal.     

Morphologic changes in the ovary and histologic changes in the tertiary ovarian follicles in ewes after prolonged irradiation during anestrus

The changes in volume, weight and the histomorphological changes of the tertiary follicles of ewes were studied after protracted irradiation with 4.8 Gy in the anoestrous period by the morphometric and qualitative histological methods. The trial was performed in May with 21 ewes of the Slovak Merino breed, divided into three groups. The first group (five ewes) was control. The second and third groups (each containing eight ewes) were exposed to gamma-rays for five days, the total dose being 4.8 Gy. Within ten days after the treatment, all the irradiated and control ewes were given Ampicillin Spofa per os at a dose of 250 mg per head/day and Roboran Spofa at a dose of 10 g per head/day. The animals were killed by bleeding on the fifth day of irradiation and on the tenth day after the end of the treatment. After killing, the volume and weight of the ovaries were determined and a common histological method was used to cut these ovaries into 7 microns slices in series 70 microns apart. The slices were stained with haematoxylin-eosine and were evaluated by means of light microscopy. After irradiation the weight of the ovaries was found to decrease significantly; however, ovary volume remained unchanged. The atretic and non-atretic tertiary follicles were subjected to qualitative histological differentiation after Marion et al. (1968) and the number of non-atretic follicles was found to have decreased significantly in the irradiated ewes. The late type of atresia contributes most significantly to an increase in the proportion of atretic tertiary follicles. The administration of vitamins after irradiation reduced the occurrence of atretic changes.     

利用多种质资源选育多抗型棉花品种的育种学启示

用选自陆海杂交后代的抗蚜选系抗７７为母本与具中棉亲缘的丰产品种江苏棉１号杂交，后代与父本江苏棉１号回交２次，经２０代综合鉴定和多性状定向选育的川棉１０９。该品种抗棉蚜，抗枯萎病和立枯病，耐黄萎病，耐旱，抗倒，耐瘠薄；并克服了陆地棉密茸毛与短纤维的连锁，将抗虫与抗病、丰产与优质有机组合；综合性状鉴定为国内领先和国际先进水平．目前己累积推广２７万ｈｍ２，是利用多质资源培育多抗型丰产品种的实例。多质资源是前提，多抗性、强生长势、强补偿力等构成了川棉１０９丰产稳产的综合遣传优势。启示育种目标日趋综合的现代棉花育种应选择宽广的质源，培育多抗性取代单抗性，并以胁迫和非胁迫双重条件下的产量比较为核心，实现抗性与丰产性的统一。     

Elevated CO₂ enhances leaf senescence during extreme drought in a temperate forest

In 2007, an extreme drought and acute heat wave impacted ecosystems across the southeastern USA, including a 19-year-old Liquidambar styraciflua L. (sweetgum) tree plantation exposed to long-term elevated (E(CO(2))) or ambient (A(CO(2))) CO(2) treatments. Stem sap velocities were analyzed to assess plant response to potential interactions between CO(2) and these weather extremes. Canopy conductance and net carbon assimilation (A(net)) were modeled based on patterns of sap velocity to estimate indirect impacts of observed reductions in transpiration under E(CO(2)) on premature leaf senescence. Elevated CO(2) reduced sap flow by 28% during early summer, and by up to 45% late in the drought during record-setting temperatures. Modeled canopy conductance declined more rapidly in E(CO(2)) plots during this period, thereby directly reducing carbon gain at a greater rate than in A(CO(2)) plots. Indeed, pre-drought canopy A(net) was similar across treatment plots, but declined to ～40% less than A(net) in A(CO(2)) as the drought progressed, likely leading to negative net carbon balance. Consequently, premature leaf senescence and abscission increased rapidly during this period, and was 30% greater for E(CO(2)). While E(CO(2)) can reduce leaf-level water use under droughty conditions, acute drought may induce excessive stomatal closure that could offset benefits of E(CO(2)) to temperate forest species during extreme weather events.     

Blind inlets: conservation practices to reduce herbicide losses from closed depressional areas

Purpose

In a 6-year study, we investigated the effectiveness of blind inlets as a conservation practice in reducing pesticide losses compared to tile risers from two closed farmed depressional areas (potholes) in the US Midwest under a 4-year cropping rotation.

Materials and methods

In two adjacent potholes within the same farm and having similar soils, a conventional tile riser and blind inlet were installed. Each draining practice could be operated independent of each other in order to drain and monitor each depression with either practice. Sampling events (runoff events) were collected from the potholes from 2008 to 2013 using autosamplers. The samples were analyzed for atrazine, metolachlor, 2,4-D, glyphosate, and deethylatrazine.

Results and discussion

The results of this study demonstrated that the blind inlet reduced analyzed pesticide losses; however, the level of reduction was compound dependent: atrazine (57 %), 2,4-D (58 %), metolachlor (53 %), and glyphosate (11 %).

Conclusions

Results from this study corroborate previous research findings that blind inlets are an effective conservation practice to reduce discharge and pollutants, including pesticides from farmed pothole surface runoff in the US Midwest.

     

Farm and Operator Characteristics Affecting the Awareness and Adoption of Precision Agriculture Technologies in the US

Precision agriculture (PA) technologies have been commercially available since the early 1990s. However, not only has the pace of adoption in the US been relatively modest but a surprisingly large number of producers are not familiar with these technologies. Using farm level survey data, this study quantifies the role that awareness plays in the decision to adopt PA technology and allows us to explore the potential for public or private information programs to affect the diffusion of PA. PA adoption and awareness are modeled as jointly determined dichotomous variables and their determinants are estimated using a two-stage (i.e. instrumental variable) logistic specification. The first-stage logit model indicated that operator education and computer literacy, full-time farming, and farm size positively affected the probability of PA awareness while the effect of age was negative. Grain and oilseed farms (i.e. corn, soybean, and small grains) and specialty crop farms (i.e. fruits, vegetables, and nuts) as well as farms located in the Heartland and Northern Great Plains regions were most likely to be aware of PA technologies. The second-stage PA adoption logit model, which included an instrumental variable to account for the endogeneity of awareness, revealed that farm size, full-time farming, and computer literacy positively influenced the likelihood of PA adoption. Grain and oilseed farms were the most likely types of farms to adopt PA as were farms in the Heartland region. Awareness, as defined in this study, was not found to be limiting the adoption of PA, suggesting that farmers for whom the technology is profitable are already aware of the technology and that a sector-wide public or private initiative to disseminate PA information would not likely have a major impact on PA diffusion.     

Environmental factors affecting the severity of pneumonia in pigs

The variable manifestations of respiratory problems in finishing pigs have led to the concept of a multiple-factor aetiology for swine respiratory disease and in particular for enzootic pneumonia. The primary and secondary agents of the disease produce their most detrimental economic effects and the highest levels of mortality and morbidity during the finishing period, when the economics of production necessitate indoor housing and intensification. This paper considers the contribution of four main groups of environmental factors to the high levels of clinical disease and lesions which are found whenever large numbers of pigs are examined at slaughter. They are meteorological factors, population and social factors, management factors and airborne pollution.