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排序方式: 共有319条查询结果,搜索用时 15 毫秒
1.
This experiment was conducted to determine the effect of ruminal dosing of a mechanical stimulating brush on rumination time, ruminal passage rate and rumen fermentation status in steers fed a concentrate diet at maintenance level. Animals were dosed three Rumen Faibu (RF) per head through the rumen fistulae (RF treatment) and not dosed (control) in a change‐over design. The organic cell wall content of the concentrate diet was 12.7% of dry matter. Daily time spent on rumination was very short in both treatments with 24 min in RF treatment and 15 min in control. The turnover rate of ruminal fluid in RF treatment was higher than that in control. There were no differences in ruminal pH and total volatile fatty acid concentration between RF treatment and control. Acetic and butyric acid concentrations were not different between the treatments. Propionic acid concentration tended to be higher in the animals on RF treatment than in control animals. The RF dosing in Holstein steers fed a low fiber diet did not affect the rumination time, but increased rumen digesta passage rate and ruminal propionic acid production. 相似文献
2.
Identification of sulfonylurea-resistant biotypes of paddy field weeds using a novel method based on their rooting responses 总被引:2,自引:0,他引:2
3.
Tsutomu MATSUMOTO Yuichiro NARA Hiromitsu FURUYA Harumi TAKAHASHI Kiichi TAIRAKO Hideki YAMAMOTO 《Journal of General Plant Pathology》2002,68(4):382-384
L11A-Fukushima (L11A-F) derived from attenuated isolate LuA of Tomato mosaic virus (ToMV) has the highest ability to cross protect against virulent ToMV among LuA and its derivatives and is stably inherited.
Growth, yield, fruit quality and symptom attenuation of inoculated tomato plants did not differ significantly between L11A-F and L11A. The infectivity of progeny viruses in tomato infected with LuA-F was less than 4% of that with virulent ToMV. From these
results, L11A-F appears to possess the properties necessary for practical use. To manage L11A-F strictly, a PCR-based assay to detect trace contamination of virulent ToMV in L11A-F preparations was established.
Received 10 June 2002/ Accepted in revised form 30 October 2002 相似文献
4.
Akihito TAKAHASHI Ajalli RAHIM Miki TAKEUCHI Emiko FUKUI Midori YOSHIZAWA Kuniaki MUKAI Makoto SUEMATSU Hidetoshi HASUWA Masaru OKABE Hiromichi MATSUMOTO 《The Journal of reproduction and development》2016,62(1):43-49
Tubulointerstitial nephritis antigen-like 1 (Tinagl1, also known as adrenocortical zonation factor 1 [AZ-1]
or lipocalin 7) is a matricellular protein. Previously, we demonstrated that Tinagl1 expression was restricted
to extraembryonic regions during the postimplantation period and detected marked expression in mouse
Reichert’s membranes. In uteri, Tinagl1 is markedly expressed in the decidual endometrium during the
postimplantation period, suggesting that it plays a physical and physiological role in embryo development
and/or decidualization of the uterine endometrium during pregnancy. In the present study, in order to
determine the role of Tinagl1 during embryonic development and pregnancy, we generated
Tinagl1-deficient mice. Although Tinagl1–/– embryos were not
lethal during development to term, homologous matings of Tinagl1–/– females and
Tinagl1–/– males showed impaired fertility during pregnancy, including failure
to carry pregnancy to term and perinatal lethality. To examine ovarian function, ovulation was induced with
equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG); the number of ovulated oocytes did
not differ between Tinagl1–/– and Tinagl1flox/flox.
In vitro fertilization followed by embryo culture also demonstrated the normal
developmental potential of Tinagl1-null embryos during the preimplantation period. Our
results demonstrate that Tinagl1 deficiency affects female mice and results in subfertility phenotypes, and
they suggest that although the potential of Tinagl1–/– oocytes is normal, Tinagl1
is related to fertility in adult females but is not essential for either fertilization or preimplantation
development in vitro. 相似文献
5.
Expression dynamics of bovine MX genes in the endometrium and placenta during
early to mid pregnancy
Takahiro SHIROZU Keisuke SASAKI Manabu KAWAHARA Yojiro YANAGAWA Masashi NAGANO Nobuhiko YAMAUCHI Masashi TAKAHASHI 《The Journal of reproduction and development》2016,62(1):29-35
MX belongs to a family of type I interferon (IFN)-stimulated genes, and the MX protein has
antiviral activity. MX has at least two isoforms, known as MX1 and
MX2, in mammals. Moreover, bovine MX1 has been found to have alternative
splice variants—namely, MX1-a and MX1B. In ruminants, IFN-τ—a type I IFN—is
temporarily produced from the conceptus before implantation and induces MX expression in the
endometrium. However, the expression dynamics of MX after implantation are not clear. In the
present study, we investigated the expression of MX1-a, MX1B and
MX2 in the endometrium and placenta before and after implantation along with the expression
of IFN-α, type I receptors (IFNAR1 and IFNAR2) and
interferon regulatory factors (IRF3 and IRF9). Pregnant uterine samples were
divided into five groups according to pregnancy days 14–18, 25–40, 50–70, 80–100, and 130–150. Tissue samples
were collected from the intercaruncular endometrium (IC), caruncular endometrium (C) and fetal placenta (P).
Although all the MX expressions were significantly higher in the IC and C at days 14–18,
presumably caused by embryo-secreted IFN-τ stimulation, their expressions were also detectable in the IC, C
and P after implantation. Furthermore, IFN-α expression was significantly higher in the IC.
RT-PCR indicated IFNAR1, IFNAR2, IRF3 and
IRF9 mRNA in all the tissues during pregnancy. These results suggest that all the
MX genes are affected by the type I IFN pathway during pregnancy and are involved in an
immune response to protect the mother and fetus. 相似文献
6.
Shunsuke Moriyama Katsuo Tashiro Suehiro Furukawa Hiroshi Kawauchi 《Fisheries Science》2008,74(4):860-866
ABSTRACT: The ability of salmon growth hormone (sGH) to accelerate the somatic growth of juvenile abalone Haliotis discus hannai by immersion was examined. After immersion for one hour into a sGH-rich solution at a concentration of 30 mg/L, the sGH immunoreactivity in the body fluid of abalone was maximal after one day, and levels were still detectable at two days. No immunoreactivity was observed in the control group. Following immersion for one hour at one or two-week intervals for 120 weeks into the sGH-rich solution at 30 mg/L, the sGH-immersed abalone exhibited a significant increase in shell length and body weight. On the other hand, abalone immersed into a salmon prolactin (sPRL)-rich solution at 30 mg/L for one hour showed a lower increase in both shell length and body weight than the sGH-immersed abalone. No increases in shell length and body weight were observed in either the bovine serum albumin-immersed abalone or untreated controls. These results provide evidence that sGH can be transported from ambient water into the circulatory system of abalone, and can subsequently improve the somatic growth of juveniles. 相似文献
7.
Tongjun Ren Shunsuke Koshio Orhan Uyan Connie Fay Komilus Saichiro Yokoyama Manabu Ishikawa Md Kadel Abdul 《Journal of the World Aquaculture Society》2008,39(6):797-803
A trial was conducted to determine the effect of ascorbyl‐2‐monophosphate Na/Ca (AMP‐Na/Ca) on blood chemistry and nonspecific immune response of red sea bream juveniles. Test diets with three levels of AsA (free, 107, and 325 mg/kg diet) were fed to juvenile red sea bream (36.0 ± 1.3 g) two times a day for 3 wk. There were no significant differences in hematocrit, glucose, and blood urea nitrogen. Total cholesterol and triglyceride in plasma of fish fed AsA‐free diet was significantly (P < 0.05) higher than that of fish fed two other diets. There were no significant differences in serum albumin, total bilirubin, and total serum protein. Glutamyl oxaloacetic transaminase in serum of fish fed diets containing 107 and 325 mg of AsA were significantly (P < 0.05) lower than that of fish fed AsA‐free diet. Serum lysozyme activity (LA) of fish fed diets containing 107 and 325 mg of AsA were significantly (P < 0.05) higher than that of fish fed AsA‐free diet. There was no significant difference in mucus LA. The results mentioned above demonstrated that AMP‐Na/Ca is a bioavailable AsA source for red sea bream juveniles. Supplement of more than 107 mg AsA/kg in diets improved blood chemistry and nonspecific immune function of red sea bream juveniles. 相似文献
8.
Yasunori SHINOZUKA Naoki SUZUKI Sohei KANEKO Kazuhiro KAWAI Tomomi KURUMISAWA Yuko SHIMIZU Tadashi IMANISHI Ayumu OHNO Mano TAKAHASHI Naoki ISOBE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(3):310
The purpose of this study was to determine the concentrations of antimicrobial components (immunoglobulin A (IgA), lactoferrin (LF), lingual antimicrobial peptide (LAP), and S100A7) in normal milk and their relation to host factors (Age, somatic cell count (SCC), days in milk, richness, and alpha diversity of the milk microbiota) in dairy cows using multivariate regression tree analyses, and to clarify how the milk microbiota is related to the obtained results. Thirty normal milk samples were collected from a commercial dairy farm in June 2020. The thresholds that predicted the concentration of each antimicrobial component in milk were obtained by regression tree analysis, and the beta-diversity of the milk microbiota composition between groups divided according to each threshold was compared by an analysis of similarities test. The IgA and LF concentrations were mainly predicted by the SCC (177,500 and 70,000 cells/ml, respectively), and the LAP and S100A7 concentrations were predicted by Age (29.667 and 40.3 months, respectively). No relationship was observed between the concentration of IgA, LAP, or S100A7 and the milk microbiota composition between the groups divided by the threshold for prediction, but the milk microbiota composition was significantly different between the groups divided by the threshold for predicting the LF concentration. Our results indicated that the LF concentration in normal milk may be associated with the milk microbiota composition. 相似文献
9.
Md. Abdul Kader Shunsuke Koshio Manabu Ishikawa Saichiro Yokoyama Mahbuba Bulbul Binh Thanh Nguyen Jian Gao Asda Laining 《Aquaculture Research》2012,43(10):1427-1438
An experiment was carried out to evaluate fermented soybean meal and squid by‐product blend (1:1) (FP) as replacement of fishmeal (FM) for Japanese flounder, Paralichthys olivaceus. Five isocaloric (19 kJ g?1) diets were prepared by replacing 0 (FP0), 12 (FP12), 24 (FP24), 36 (FP36) and 48% (FP48) FM protein with FP. Triplicate groups of juveniles (mean weight of 3.9 g) were delivered the test diets for 8 weeks in a flow‐through sea water system. The results showed that there were no significant differences (P > 0.05) among the growth rates of fish fed FP0, FP12, FP24 and FP36 diets. Growth and nutrient utilization parameters were significantly reduced in fish fed FP48 diet. Although, whole body proximate composition of fish were not significantly affected by the dietary treatments compared to the control; methionine and phenylalanine contents were significantly decreased in FP48 group. Protein retention was also significantly decreased in the similar group of fish. Dietary treatments did not alter most of the plasma metabolites, while some of the health parameters were improved in the replacement groups. Results suggested that FP is a potential candidate for alternative protein ingredient in aquafeed and can replace 36% FM protein in the diet of Japanese flounder. 相似文献
10.
Effect of high inclusion of rendered animal by‐product ingredients on growth,digestibility and economic performances in climbing perch Anabas testudineus
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Md Sakhawat Hossain Md Abdul Kader Tanwi Dey Nadia Mahjabin Sony Mahbuba Bulbul Shunsuke Koshio 《Aquaculture Research》2017,48(3):931-940
A 70‐day growth trail was conducted to investigate the effects of inclusion of high levels of meat and bone meal (MBM) and protein concentrate (PC) on growth, digestibility and economic performances of climbing perch, Anabas testudineus. Four isonitrogenous diets were formulated by lowering the level of dietary fishmeal protein at 0 (D1, control), 70 (D2), 85 (D3) and 100% (D4) with a mixture of MBM and PC protein (1:1). Triplicate groups of 300 fish (mean weight of 0.80 g) stocked in each 40 m2 pond and fed the respective test diets. A digestibility trial was conducted after the growth trial in indoor glass aquarium. The result showed that growth parameters were significantly decreased (P < 0.05) with fishmeal replacement levels. However, significant differences were not found in feed conversion ratio and survival of fish. No difference was also found in protein efficiency ratio among D1, D2 and D3. Similar to growth parameters, total fish production was highest in D1, intermediate in D2 and D3; and lowest in D4. Apparent digestibility coefficients of dry matter, protein and lipid were highest (P < 0.05) in D1 and lowest in D4. The economic analysis revealed that the benefit cost ratio was ranked by D3 (1.81), D2 (1.71), D1 (1.66) and D4 (1.46) respectively. Upon considering the overall performances and unavailability of finite protein sources, it can be concluded that 70–85% fishmeal could be replaced with a mixture of MBM and PC (1:1) in practical diets for climbing perch. 相似文献