排序方式: 共有11条查询结果,搜索用时 15 毫秒
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Noboru SASAKI Thandar OO Yoshikazu YASUDA Takahiro ICHISE Noriyuki NAGATA Nozomu YOKOYAMA Kazuyoshi SASAOKA Keitaro MORISHITA Shouta MM NAKAYAMA Mayumi ISHIZUKA Kensuke NAKAMURA Mitsuyoshi TAKIGUCHI Yoshinori IKENAKA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1634
We developed an analytical method using an on-line column-switching liquid chromatography with triple quadrupole mass spectrometry (LC/MS/MS) for quantifying multiple steroids in serum. Using the developed method, we evaluated the serum concentration of nine steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, 21-deoxycortisol, deoxycorticosterone, progesterone, 17α-OH-progesterone and aldosterone) in dogs with hyperadrenocorticism (HAC). Serum was mixed with stable isotope internal standards and thereafter purified by the automated column-switching system. The limit of detection ranged 2–16 pg/ml for nine steroids. In the baseline samples, five steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, and 17α-OH-progesterone) were detected in all dogs. The concentrations of cortisone, 11-deoxycortisol, and 17α-OH-progesterone in dogs with HAC (n=19) were significantly higher those in dogs without HAC (n=15, P<0.02). After the adrenocorticotropic hormone stimulation test, six steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, 17α-OH-progesterone, and deoxycorticosterone) were above the limit of quantification in all dogs. Cortisol, corticosterone, cortisone, and deoxycorticosterone concentrations of dogs with HAC were significantly higher than those of dogs without HAC (P<0.02). In addition, 11-deoxycortisol and 17α-OH-progesterone concentration was higher in dogs with HAC than in dogs without HAC (P=0.044 and P=0.048, respectively). The on-line column-switching LC/MS/MS would be feasible for measuring multiple steroids in dog serum. The results suggest that cortisone, 11-deoxycortisol, and 17α-OH-progesterone would be related to HAC. Further studies are warranted to assess the clinical feasibility of steroid profile in dogs with HAC. 相似文献
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Andrew KATABA Shouta M.M. NAKAYAMA Yared Beyene YOHANNES Haruya TOYOMAKI Hokuto NAKATA Yoshinori IKENAKA Mayumi ISHIZUKA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1674
Lead (Pb) exposure occurs together with other metals including zinc (Zn). This study investigated the impact of Zn on Pb tissue accumulation and Pb-induced toxicities. Animals (n=6 rats per group) were exposed to lead acetate (PbAc) or a combination of PbAc and zinc acetate (ZnAc) under the following groups: control (deionized water), low PbAc [12 mg/kg PbAc (3 mg PbAc/rat/day)], low PbAc–ZnAc [12 mg/kg PbAc (3 mg PbAc/rat/day) + 0.2 mg ZnAc/rat/48 hr], high PbAc [120 mg/kg (30 mg PbAc/rat/day)], and high PbAc–ZnAc [120 mg/kg (30 mg PbAc/rat/day) + 1 mg ZnAc/rat/48 hr] for 8 weeks. A significant reduction in body weight gain was observed in the high PbAc group relative to the control group. Muscles and testes both had reduced and increased Pb uptake in low PbAc–ZnAc and high PbAc–ZnAc groups compared to PbAc only groups, respectively. Bone Pb levels in the high PbAc–ZnAc group were lower than the high PbAc group. Zinc co-administration attenuated Pb-induced inhibition of delta aminolaevulinic acid dehydratase enzyme and enhanced catalase enzyme activity at a high level of exposure. Moreover, ZnAc seems to have minimized the effects of Pb-induced mRNA dysregulation in antioxidant and antiapoptotic enzymes encoding genes. Heme oxygenase-1 was downregulated in the kidney and brain in the low PbAc group. Liver glutathione peroxidase and thioredoxin reductase-1 were downregulated in the high PbAc group. These findings suggest that zinc co-administration with lead may partially mitigate against Pb-induced toxicities. 相似文献
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Shinji Sasaki Eiji Muraki Yoshinobu Inoue Ryouhei Suezawa Hideki Nikadori Yuuichi Yoshida Shouta Nariai Ryoya Hideshima Shiyunsuke Moriwaki Ryotaro Nakashima Katsuo Uchiyama Kanako Yoshinari Masayuki Takeda Takatoshi Kojima 《Animal Science Journal》2019,90(12):1503-1509
Single nucleotide polymorphism (SNP) arrays are widely used for genetic and genomic analyses in cattle breeding; thus, data derived from SNP arrays have accumulated on a large scale nationwide. Commercial SNP arrays contain a considerable number of unassigned SNPs on the chromosome/position on the genome; these SNPs are excluded in subsequent analyses. Notably, the position‐unassigned SNPs, or “buried SNPs” include some of the markers associated with genetic disease. In this study, we identified the position of buried SNPs using the Basic Local Alignment Search Tool against the surrounding sequences and characterized the relationship between SNPs and genetic diseases in Online Mendelian Inheritance in Animals based on the genomic position. We determined the position of 285 buried SNPs on the genome and surveyed the genotype and allele frequencies of these SNPs in 5,955 individual Japanese Black cattle. Eleven SNPs associated with genetic disease, which contained five buried SNPs, were found in the population with the risk allele frequency ranging from 0.00008396 to 0.46. These results indicate that buried SNPs in the bovine SNP array can be utilized to identify associations with genetic disorders from large scale accumulated SNP genotype data in Japanese Black cattle. 相似文献
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Aksorn SAENGTIENCHAI Yoshinori IKENAKA Wageh Sobhy DARWISH Shouta M.M. NAKAYAMA Hazuki MIZUKAWA Mayumi ISHIZUKA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1261-1267
Pyrene (PY) is a polycyclic aromatic hydrocarbon (PAH) that is often used as a biomarker
for human and wildlife exposure to PAHs. As the metabolites of PAHs, similar to their
parent compounds, pose public health risks, it is necessary to study their characteristics
and tissue-specific distribution. The present study was performed to experimentally
characterize PY metabolites and analyze the tissue-specific distribution of the conjugated
metabolites after oral administration of PY to rats. PY metabolites, such as
pyrenediol-disulfate (PYdiol-diS), pyrenediol-sulfate (PYdiol-S), pyrene-1-sufate (PYOS),
pyrene-1-glucuronide (PYOG) and 1-hydroxypyrene (PYOH), were detected in rat urine.
Although glucuronide conjugate was the predominant metabolite, the metabolite composition
varied among tissues. Interestingly, the proportion of PYOH was high in the large
intestine. Furthermore, PYOH was the only PY metabolite detected in feces. 相似文献
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Misaki NISHI Shouta SUGIO Tetsushi HIRANO Daisuke KATO Hiroaki WAKE Asuka SHODA Midori MURATA Yoshinori IKENAKA Yoshiaki TABUCHI Youhei MANTANI Toshifumi YOKOYAMA Nobuhiko HOSHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(4):585
Neonicotinoid pesticides (NNs) cause behavioral abnormalities in mammals, raising concerns about their effects on neural circuit activity. We herein examined the neurological effects of the NN clothianidin (CLO) by in vivo Ca2+ imaging using two-photon microscopy. Mice were fed the no-observed-adverse-effect-level (NOAEL) dose of CLO for 2 weeks and their neuronal activity in the primary somatosensory cortex (S1) was observed weekly for 2 weeks. CLO exposure caused a sustained influx of Ca2+ in neurons in the S1 2/3 layers, indicating hyperactivation of neurons. In addition, microarray gene expression analysis suggested the induction of neuroinflammation and changes in synaptic activity. These results demonstrate that exposure to the NOAEL dose of CLO can overactivate neurons and disrupt neuronal homeostasis. 相似文献
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Wageh Sobhy DARWISH Yoshinori IKENAKA Alaa Eldin MORSHDY Kamal Ibrahim ELDESOKY Shouta NAKAYAMA Hazuki MIZUKAWA Mayumi ISHIZUKA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):351-354
The aim of this study was to estimate total carotenoids, β-carotene and retinol concentrations in the livers
and muscles of some ungulates (cattle, buffalo, sheep, goats and horses) in comparison with the Wistar rats as
a control. Cattle and horses had the highest contents of total carotenoids and β-carotene. Unexpectedly, sheep
was the highest accumulator of retinol with a mean concentration of 203 ± 23.34 µg/g, while
the least accumulator was buffalo, having a mean value of 58.28 ± 13.77 µg/g. Livers had
higher contents of the examined phytochemicals than muscles. Consumption of these tissues may provide human
with some needs from these important phytochemicals, though ingestion of livers, especially that of the sheep,
is not advisable for the pregnant women. 相似文献
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