Hormone-sensitive lipase: sequence, expression, and chromosomal localization to 19 cent-q13.3

Hormone-sensitive lipase, a key enzyme in fatty acid mobilization, overall energy homeostasis, and possibly steroidogenesis, is acutely controlled through reversible phosphorylation by catecholamines and insulin. The 757-amino acid sequence predicted from a cloned rat adipocyte complementary DNA showed no homology with any other known lipase or protein. The activity-controlling phosphorylation site was localized to Ser563 in a markedly hydrophilic domain, and a lipid-binding consensus site was tentatively identified. One or several messenger RNA species (3.3, 3.5, or 3.9 kilobases) were expressed in adipose and steroidogenic tissues and heart and skeletal muscle. The human hormone-sensitive lipase gene mapped to chromosome 19 cent-q13.3.     

Ocular lesions associated with coccidioidomycosis in dogs: 35 cases (1980-1985)

Thirty-five dogs with ocular lesions associated with coccidioidomycosis were examined. Serologic and/or histologic evaluation confirmed the diagnoses of coccidioidomycosis. The disease most frequently associated with signs referable to the anterior segment of the eye, such as iritis and granulomatous uveitis. Histologic evaluation of the eye revealed a primary posterior segment disease, such as chorioretinitis or retinal separation, with an extension into the anterior segment of the eye.     

Polycystic ovary syndrome with endometrial hyperplasia in a cynomolgus monkey (Macaca fascicularis)

A 13-year-old, obese, female cynomolgus monkey (Macaca fascicularis) was observed in a 5-year neurobehavioral study and was humanely euthanatized for experimental purposes. During this observational study, the monkey was noted to ovulate only rarely (0-3 times a year), with a prolonged menstrual cycle length (up to 161 days), hyperandrogenism (androstenedione area under the curve in response to adrenocorticotropic hormone up to 27.64 ng/ml), and hyperinsulinemia (fasting insulin up to 65.85 microIU/ml). This animal's body mass index was 65.46 kg/m(2), with central obesity. On postmortem examination, the uterus was moderately enlarged, with an eccentric lumen and a broad-based endometrial polyp that consisted of complex glandular hyperplasia with atypia. Both ovaries contained many 2- to 3-mm follicles, without any corpora lutea. A diagnosis of polycystic ovary syndrome was made based on the clinical history, endocrinology, and gross and histopathologic findings.     

Ocular coccidioidomycosis in a cat

Enucleation of the right eye was performed on a 12-year-old male Persian cat when therapy for uveitis failed. Histologic examination of the anterior and posterior chambers and the vitreous led to a diagnosis of endophthalmitis caused by Coccidioides immitis infection. The primary focus of infection was not determined. Latex particle agglutination, agar gel immunodiffusion, and complement fixation gave negative results for Coccidioides immitis antibody.     

Carbon sequestration in a tropical landscape: an economic model to measure its incremental cost

Farm level rates of carbon sequestration are derived for timber and agroforestry systems based on Paraserianthes falcataria. An economic model is used to measure the incremental cost of carbon storage, based on the opportunity cost of land diverted from annual crop production. The method is applied to the Manupali watershed, in the Philippine province of Bukidnon, to estimate carbon storage potential and carbon storage costs at a landscape scale. Carbon storage via land use modification is calculated to cost between $3.30 per ton on fallowed lands and $62.50 per ton on land that otherwise supports high value cropping. Carbon storage through agroforestry is less costly than via a pure tree-based system; a strong argument for the role of agroforestry rather than forestry per se, in re-forestation projects. This revised version was published online in June 2006 with corrections to the Cover Date.