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Shiokawa Kanae Welcome Shamara Kenig Michalina Lim Brenda Rajeev Sreekumari 《Tropical animal health and production》2019,51(6):1645-1650
Tropical Animal Health and Production - This pilot study describes the prevalence of Leptospira infection and exposure in livestock species, cattle, pig, sheep, and goats in Saint Kitts in the... 相似文献
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Retinal dysplasia in American pit bull terriers – phenotypic characterization and breeding study
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Shiokawa T Yamada S Futamura N Osanai K Murasugi D Shinohara K Kawai S Morohoshi N Katayama Y Kajita S 《Tree physiology》2008,28(1):21-28
We report the isolation and characterization of CjNdly, a homolog in Japanese cedar (Cryptomeria japonica D. Don) of the FLORICAULA/LEAFY (FLO/LFY) genes. We determined the entire nucleotide sequence of CjNdly, including short 5'- and 3'-untranslated regions. The deduced amino acid sequence was similar to those of the products of the FLO/LFY genes from other species. The nucleotide sequence showed the closest homology to that of the NEEDLY gene in Pinus radiata D. Don. Although no proline-rich region has been reported previously in homologous gene products from gymnosperms, we found such a region at the amino-terminal end of the deduced amino acid sequence encoded by CjNdly. We detected the expression of CjNdly in both reproductive and vegetative tissues and organs of C. japonica. Heterologous expression of CjNdly in transgenic tobacco plants induced precocious flowering of regenerating shoots on agar-solidified medium and flowers with an abnormal phenotype, namely, petal-like stamens. Our findings suggest that the CjNdly gene may have important roles in flower development in Japanese cedar, resembling those of its angiosperm homologs. 相似文献
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