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Hiroshi ISEKI Michihiro TAKAGI Kenji KAWASHIMA Tomoyuki SHIBAHARA Yoshiko KURODA Hiroshi TSUNEMITSU Makoto YAMAKAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1663-1666
To clarify the pathogenicity of Japanese type 1 porcine reproductive and respiratory
syndrome virus (PRRSV) isolate in experimentally infected pigs, we evaluated clinical
signs and monitored viremia for 21 days post-inoculation (dpi). Lungs were mottled, tanned
and reddish in appearance; had lesions predominantly in the cranial, middle and accessory
lobes; and failed to collapse at 10 dpi. Although microscopic lesions of lungs were
reproduced using the Japanese emerging type 1 PRRSV isolate under experimental conditions,
no significant differences were noted between the challenge and control groups regarding
mean rectal temperature and daily weight gain. These results provide useful insights into
the limited pathogenicity of single infection with the Japanese type 1 PRRSV isolate in
piglets, which differ from findings in reported field cases. 相似文献
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WADA T TAKAGI H MINAKAMI H HAMANAKA W OKAMOTO K ITO A SAHASHI Y 《Science (New York, N.Y.)》1961,134(3473):195-196
S-Benzoylthiamine O-monophosphate has been synthesized, and its physicochemical and biological properties have been investigated. It is a stable crystalline substance, it exerts thiamine activity approximately equivalent to that of thiamine hydrochloride in thiamine-requiring microorganisms, and it is easily absorbed in organisms, particularly by oral administration. 相似文献
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Masamitsu TOMIYAMA Shouhei KUBO Tsutomu TAKAGI Keiichi SUZUKI 《Animal Science Journal》2011,82(5):621-626
Genetic improvement of the reproductive performance of pigs is important for pig breeding despite their low heritabilities. The objectives of this study were to investigate the effectiveness of selection concerning reproductive traits and to determine the optimal number of parity records required for accurate estimation of breeding values (BVs) in the open population of a commercial pig breeding company. The study used records of 2220 purebred Large White pigs (9845 litters) farrowed between 1998 and 2009 in the two herds of the Pacific Ocean Breeding Co. Ltd. The traits studied included farrowing interval (FI), total number of piglets at birth (TNB), average weaning weight per litter (AWW), and raising rate (RR). A statistical model was applied to the 4‐trait repeatability animal model. The heritabilities of FI, TNB, AWW and RR were low. The genetic trends in TNB (h2 = 0.09) showed approximately 1.0 increase in 6 years from 2003 to 2008. The predicted error variances indicated that up to fourth parity records are necessary for accurate genetic evaluation. The present study results indicated that even reproductive traits with low heritability can be improved. 相似文献
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Shiaki TAKAGI Shunji HAYASHI Koichi TAKAHASHI Hiroshi ISOGAI Lanlan BAI Hiroshi YONEYAMA Tasuke ANDO Kumiko ITO Emiko ISOGAI 《Animal Science Journal》2012,83(6):482-486
A bovine myeloid antimicrobial peptide (BMAP‐28) is a member of the cathelicidin family which is included in the innate immune system of mammals. Recently, there have been many studies about antimicrobial peptides. This study aims to clarify whether BMAP‐28 has bactericidal activity against methicillin‐resistant Staphylococcus aureus (MRSA) and compares its activity against methicillin‐susceptible S. aureus (MSSA) and MRSA. We found that the peptide was effective in killing MRSA (minimal inhibitory concentration (MIC) range; 5–20 µg/mL). It was also revealed that MSSA (MIC range; 1.25–20 µg/mL) had two levels of susceptibility to BMAP‐28. We also examined the effect of BMAP‐28 on bacterial shape to visually show its activity. After exposure to the peptide, both MSSA and MRSA cells showed the morphological changes on their surfaces. Our results indicate that BMAP‐28 is a promising candidate for medicine against drug‐resistant bacteria. 相似文献
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Ryo TAKEUCHI Satoshi TAKAGI Kenji HOSOYA Hiroshi OHTA Takaharu ITAMI Masahiro OKUMURA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1740
Canine gastroesophageal intussusception (GEI) is a rare and life-threatening condition that requires prompt diagnosis and treatment. A 19-day-old Siberian Husky with a 4-day history of regurgitation was diagnosed with GEI based on the findings of computed tomography (CT) performed without anesthesia. Endoscopic reduction of intussusception was impossible; thus, surgical reduction by traction of the duodenum was performed. CT revealed improvement of megaesophagus 82 days postoperatively. Eleven months postoperatively, fluoroscopy showed recovery to nearly normal esophageal motility. Two years postoperatively, no clinical signs were reported. CT is useful to diagnose GEI in neonate puppies with poor abdominal fat and to assess the gastric edema and the anatomical association of stomach with other organs. Fluoroscopy is helpful for evaluating postoperative esophageal motility. 相似文献
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Md Shafiqul ISLAM Urara SHINYA Mitsuhiro TAKAGI Takao AKAHOSHI Akira YABUKI Shahnaj PERVIN Tofazzal Md RAKIB Mohammad Mahbubur RAHMAN Martia Rani TACHARINA Osamu YAMATO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(2):254
Bovine isoleucyl-tRNA synthetase (IARS) disorder, a major cause of weak calf syndrome, is caused by a homozygous missense (c.235G>C) mutation in the bovine IARS gene of Japanese Black (JB) cattle, which was identified in 2013. However, the extent to which the carrier rate has changed at Kagoshima prefecture, Japan, and whether the carrier status is associated with any clinical or reproductive problems, have yet to be ascertained. In this study, using a real-time polymerase chain reaction-based genotyping assay, we determined the carrier rate in a regional JB cow population at Kagoshima prefecture. Comparative analyses were performed on the metabolic profile test (MPT) results and reproductive performance data obtained for heterozygous carrier and homozygous wild-type cows. In 2009 and 2018, DNA samples were collected from 130 and 462 clinically healthy JB cows, respectively, in Kagoshima prefecture. MPT results and reproductive performance data were evaluated for 62 cows, comprising four heterozygous carriers and 58 wild-type cows. Genotyping revealed that the carrier rate was 6.9% in 2009 and 1.5% in 2018, the difference of which was statistically significant (P<0.005). There were no statistically significant differences between the carrier and wild-type cows with respect to either MPT results or reproductive performance, indicating that the carrier cows have necessary IARS activity to maintain minimal health and reproductive potential. 相似文献
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