Cell wall disruption: An effective strategy to improve the nutritive quality of microalgae in African catfish (Clarias gariepinus)

The rigid cell walls of microalgae may hinder their utilization in fish feeds. The current experiment assessed the correlation between the accessibility of microalgae nutrients and their in vivo digestibility in African catfish. Nannochloropsis gaditana biomass was subjected to physical or mechanical treatments to weaken its cell wall; untreated—no disruption treatment (UNT), pasteurization (PAS), freezing (FRO), freeze‐drying (FRD), cold pasteurization (L40) and bead milling (BEM). Six experimental diets formulated from differently treated and untreated microalgae (at 30% diet inclusion level) were tested on growth performance and apparent nutrient digestibility (ADCs) in juvenile African catfish. A basal diet (REF) containing no microalgae was used as reference diet. Results showed that biomass gain and feed conversion ratio of fish fed L40 and BEM diets increased by 13% and 11%, respectively, relative to the UNT diet. Additionally, FRD, FRO, L40 and BEM cell wall disruption treatments improved protein digestibility by 0.5%, 5.9%, 8.4% and 16.3%, respectively, compared to the UNT treatment. There was a positive correlation between accessibility of microalgal nutrients and their digestibility in African catfish. Nutrient digestibility of microalgae was dependent on extent of cell disruption. Also, the impact of cell disruption on nutrient digestibility of microalgae differs between African catfish and Nile tilapia.     

Use of a laryngeal mask airway in rabbits during isoflurane anesthesia

The purpose of this study was to find out if an LMA (#1 LMA‐Classic) would provide a better airway than a face mask in spontaneously breathing anesthetized rabbits, and to test if it could be used for mechanically controlled ventilation. Sixteen rabbits (4.1 ± 0.8 kg, mean ± SD) were assigned randomly to three treatment groups; face mask with spontaneous ventilation (FM‐SV; n = 5), LMA with spontaneous ventilation (LMA‐SV; n = 5), and LMA with controlled ventilation (LMA‐CV; n = 6). Rabbits were anesthetized in dorsal recumbency using a circle circuit at constant ET isoflurane (2.3%, Datex airway gas monitor) and constant rectal temperature (38.85 °C) for 2 hours. PaCO₂, PaO₂, minute volume, tidal volume (Wright's respirometer), and Pe CO₂ were measured at 15 minute intervals. Two individuals in the FM‐SV group had PaCO₂ >100 mm Hg (>13.3 kPa). One rabbit in the FM‐SV had PaO₂ <80 mm Hg (<10.7 kPa). All FM‐SV rabbits showed signs of airway obstruction and two were withdrawn from the study at 45 and 90 minutes, respectively, because of cyanosis. Tidal volume could not be measured in the FM‐SV group. No signs of airway obstructions were observed in either of the LMA groups. Four rabbits in the LMA‐CV group developed gastric tympany, and one of these refluxed after 110 minutes. The significance of differences between the two spontaneously breathing groups and between the two LMA groups were measured using Wilcoxon's rank sum test (with significance assumed at p < 0.05). There were no statistical differences between FM‐SV and LMA‐SV in any variable tested. PaCO₂ and Pe ′CO₂ were less in the LMA‐CV group than in the LMA‐SV group, while PaO₂, tidal volume, and minute volume were all more. We conclude that biologically, the LMA provides a better airway than the face mask during spontaneous breathing and that it can be used for IPPV, but that gastric tympany is likely to occur during IPPV.     

Digestibility, faeces recovery, and related carbon, nitrogen and phosphorus balances of five feed ingredients evaluated as fishmeal alternatives in Nile tilapia, Oreochromis niloticus L.

This study shows that alternatives for fishmeal in a fish diet affect not only fish growth but also faeces stability and nitrogen (N) and phosphorus (P) waste production. Wheat gluten diet (WGD), soybean meal extract diet (SBE), soybean meal diet (SBM), duckweed diet (DWD) and single‐cell protein diet (SCP) were evaluated as a fishmeal replacement on a 15% weight weight^?1 basis in tilapia diets. Fishmeal replacement affected dry matter (dm), protein, ash and P digestibility significantly. Faeces recovery (6.8–11.2%) was not significantly affected, although the amount of non‐recovered faeces and total faeces showed significant differences. Duckweed diet and SCP resulted in the largest amounts of non‐recovered and total faeces (199–210, 224–225 g dm kg^?1 feed dm). Compared with fishmeal diet (FMD), the WGD and SBE resulted in similar growth, but higher non‐faecal N losses (471–495 vs. 416 g N kg^?1 N). Soybean meal diet, DWD and SCP resulted in lower growth but less non‐faecal loss (409–450 g N kg^?1 N). The DWD and FMD had the highest N retention (480 g N kg^?1 N) compared with the other diets (431–451 g N kg^?1 N). Carbon retention, faecal and non‐faecal losses and P retention were similar for all diets (302–358, 142–176 and 489–523g C kg^?1 C, 606–704 g P kg^?1 P). Phosphorus faecal loss was lower for all diets (329–381 g P kg^?1 P) than for the FMD (401 g P kg^?1 P).     

Geosmin depuration from European eel (Anguilla anguilla) is not affected by the water renewal rate of depuration tanks

This study established that geosmin depuration from European eel is not affected by the water renewal rate of depuration tanks. A general fish bioaccumulation model extended with terms that account for effects of tank water renewal rate and system losses of chemicals, predicted strong effects of the water renewal rate of depuration tanks on geosmin depuration from European eel. Model predictions were validated in a depuration experiment with geosmin‐loaded European eel (n = 95) with a mean (SD) individual weight of 134.4 (5.0) g and a mean (SD) lipid content of 33.7 (2.8) % (w/w). Fish were depurated for 24, 48 or 72 h at three different tank water renewal rates (0.3, 3.3 and 33 day^?1). Treatments were installed by three different mean (SD) water flow rates (13.8 (1.3), 143.5 (9.2) and 1511 (80) L kg fish^?1 day^?1) over 30‐L tanks. Eels eliminated geosmin from their bodies, but unlike the model predicted, this was independent of the water renewal rate of the depuration tanks. Although being eliminated from the fish, geosmin hardly appeared and certainly did not accumulate in the water of the depuration tanks as the model predicted. This observation may be explained by geosmin being eliminated from eel as metabolite rather than the parent compound. Geosmin elimination from eel seems not to occur according to the generally accepted passive diffusion mechanism for excretion of lipophilic chemicals, and geosmin biotransformation by the eel seems indicated. Clearly geosmin depuration from European eel cannot be enhanced by increasing water renewal rates of depuration tanks.     

Effects of dietary fermentable carbohydrates on energy metabolism in group-housed sows

The effect of dietary nonstarch polysaccharide (NSP) content on the metabolic rate in group-housed sows was studied. Twelve groups of six nonpregnant sows were each fed one of four experimental diets similar in composition except for the starch and NSP content. Exchanging sugar beet pulp silage (SBPS) for tapioca created the difference in starch and NSP ratio in the diet. On a DM basis, diets contained 0, 10, 20, or 30% SBPS. Sows were group-housed and fed at 1.30 times the assumed maintenance energy requirements. Nitrogen and energy balances were measured per group during a 7-d experimental period, which was preceded by a 33-d adaptation period. Both digestibility and metabolizability of energy decreased with increasing dietary SBPS content (P < 0.05). Heat production and energy retention were unaffected by the exchange of starch for NSP (P > 0.1). Based on energy retention data and apparent fecal digestibilities of crude protein, crude fat, starch, and NSP, the estimated net energy value of fermented NSP was 13.4 kJ/g. The present study shows that group-housed sows are capable of using energy from fermented NSP (i.e., NSP from SBPS) as efficiently as energy from digested starch (i.e., starch from tapioca).     

Effects of dietary fermentable carbohydrates on behavior and heat production in group-housed sows

The effects of dietary nonstarch polysaccharides (NSP) on behavior and heat production in group-housed sows were studied. Twelve groups of six nonpregnant sows were fed one of four experimental diets that were similar in composition except for starch and NSP contents. Exchanging sugar beet pulp silage (SBPS) for tapioca created the difference in dietary starch and NSP ratio. On a dry matter (DM) basis, diets contained 0, 10, 20, or 30% SBPS. Sows were group-housed. Intake of fermentable NSP (fNSP) for diets containing 0, 10, 20, or 30% SBPS averaged 7.06, 9.18, 11.61, and 13.73 g x kg(-0.75) d(-1), respectively. Sows were fed, once a day at 0800. Dry matter intake for diets containing 0, 10, 20, or 30% SBPS, averaged 38.05, 38.38, 38.53, and 38.35 g x kg(-075) x d(-1), respectively, and ME intake averaged 523, 518, 514, and 493 kJ x kg(-0.75) x d(-1), respectively. On average, sows spent 177 min/d on physical activity, of which 8.8% was spent on eating. Time spent in physical activity was affected by diet (P = 0.005). Sows fed 0 or 10% SBPS spent more time on physical activity than sows fed 20 or 30% SBPS (P = 0.002). Energy cost of physical activity averaged 464 kJ x kg(-0.75) x d(-1) (standard estimated mean of 31) and was similar for diets (P = 0.679). Total heat production (HP) and activity-related heat production (AHP) were affected by diet (P < 0.05). Sows tended to be quieter when fNSP intake increased (P = 0.063). The effect of fNSP intake on HP and AHP was not constant during the day. During the night period, fNSP intake did not affect HP and AHP (P > 0.10). During the day period, increased fNSP intake decreased HP (P = 0.006) and tended to decrease AHP (P = 0.062). During eating, increased fNSP intake increased HP (P = 0.012) and tended to increase AHP (P = 0.074). Despite similar DMI, sows fed 0 or 10% SBPS spent less time eating than sows fed 20 or 30% SBPS (P = 0.009). Feed consumption rate was higher (P = 0.003) in groups fed 0 or 10% SBPS than in groups fed 20 or 30% SBPS. Feed consumption rate decreased by 0.19 g DM x kg(-0.75). min(-1) (P = 0.003) for each gram of fNSP intake. The energy saving effect of physical activity on the NE value of fNSP from SBPS ranged between 2.3 and 3.7 kJ/g of fNSP intake. In conclusion, intake of fNSP from SBPS affected energy expenditure for physical activity (P = 0.063); however, this effect was not constant during the day.     

A prolonged photoperiod improves feed intake and energy metabolism of weanling pigs

In a 2-wk experiment, the effect of photoperiod on performance and energy metabolism of newly weaned pigs was studied. Forty 4-wk-old crossbred weanling barrows weighing 8.0 kg (SE = 0.13) were assigned to one of eight groups (five pigs per group) based on BW and litter. Groups were allotted to one of two lighting schedules: 8 h light:16 h darkness or 23 h light:1 h darkness. Each group was housed in a climate respiration chamber. Piglets had ad libitum access to feed and water. Energy and nitrogen balances, heat production, ADFI, and ADG were measured weekly. Heat production, energy metabolism, and performance were unaffected (P > 0.10) by photoperiod during wk 1. However, in the 2nd wk ADFI (418 vs 302 g/d) and ADG (381 vs 240 g/d) were higher (P < 0.05 and P = 0.05, respectively) for pigs on the 23:1 h lighting schedule than for those on the 8:16 h schedule. Furthermore, heat production (P < 0.10), total energy retention, and energy retained as protein and as fat were higher (P < 0.05) during wk 2 in pigs on the 23:1 h lighting schedule (8, 125, 41, and 350%, respectively) than in those on the 8:16 h schedule. Moreover, metabolizability of energy tended to be higher (P < 0.10) and energy requirements for maintenance were lower (P < 0.05) during wk 2 for pigs on the 23:1 h schedule compared with those on the 8:16 h schedule (P < 0.10). In conclusion, exposing pigs to a longer period of light after weaning stimulated ADFI and ADG. In addition to the feed intake, the high ADG is due to an improved metabolizability of energy and a reduced energy requirement for maintenance. This study suggests that lighting schedule can be used as a tool to stimulate feed intake after weaning.     

Tracking the long-term decline and recovery of an isolated population

Effects of small population size and reduced genetic variation on the viability of wild animal populations remain controversial. During a 35-year study of a remnant population of greater prairie chickens, population size decreased from 2000 individuals in 1962 to fewer than 50 by 1994. Concurrently, both fitness, as measured by fertility and hatching rates of eggs, and genetic diversity declined significantly. Conservation measures initiated in 1992 with translocations of birds from large, genetically diverse populations restored egg viability. Thus, sufficient genetic resources appear to be critical for maintaining populations of greater prairie chickens.