全文获取类型
收费全文 | 2289篇 |
免费 | 191篇 |
国内免费 | 4篇 |
专业分类
林业 | 92篇 |
农学 | 42篇 |
基础科学 | 11篇 |
382篇 | |
综合类 | 280篇 |
农作物 | 78篇 |
水产渔业 | 129篇 |
畜牧兽医 | 1277篇 |
园艺 | 75篇 |
植物保护 | 118篇 |
出版年
2024年 | 6篇 |
2023年 | 34篇 |
2022年 | 39篇 |
2021年 | 84篇 |
2020年 | 90篇 |
2019年 | 101篇 |
2018年 | 80篇 |
2017年 | 83篇 |
2016年 | 58篇 |
2015年 | 65篇 |
2014年 | 87篇 |
2013年 | 106篇 |
2012年 | 179篇 |
2011年 | 213篇 |
2010年 | 99篇 |
2009年 | 103篇 |
2008年 | 151篇 |
2007年 | 138篇 |
2006年 | 129篇 |
2005年 | 106篇 |
2004年 | 98篇 |
2003年 | 85篇 |
2002年 | 72篇 |
2001年 | 18篇 |
2000年 | 20篇 |
1999年 | 15篇 |
1998年 | 13篇 |
1997年 | 19篇 |
1996年 | 10篇 |
1995年 | 14篇 |
1994年 | 4篇 |
1993年 | 12篇 |
1992年 | 10篇 |
1991年 | 12篇 |
1990年 | 12篇 |
1989年 | 9篇 |
1988年 | 7篇 |
1986年 | 5篇 |
1985年 | 7篇 |
1984年 | 5篇 |
1983年 | 5篇 |
1982年 | 7篇 |
1981年 | 4篇 |
1980年 | 4篇 |
1979年 | 9篇 |
1978年 | 5篇 |
1977年 | 4篇 |
1973年 | 7篇 |
1972年 | 4篇 |
1969年 | 4篇 |
排序方式: 共有2484条查询结果,搜索用时 15 毫秒
1.
Three-week-old weaned and colostrum-deprived neonatal (less than 1 day old) pigs were inoculated to determine the pathogenicity of 2 enterotoxigenic Escherichia coli isolates that do not express K88, K99, F41, or 987P adhesins (strains 2134 and 2171). Strains 2134 and 2171 were isolated from pigs that had diarrhea after weaning attributable to enterotoxigenic E coli infection. We found that both strains of E coli adhered in the ileum and caused diarrhea in pigs of both age groups. In control experiments, adherent bacteria were not seen in the ileum of pigs less than 1 day old or 3 weeks old that were noninoculated or inoculated with a nonpathogenic strain of E coli. These control pigs did not develop diarrhea. Antisera raised against strains 2134 and 2171 and absorbed with the autologous strain, grown at 18 C, were used for bacterial-agglutination and colony-immunoblot assays. Both absorbed antisera reacted with strains 2134 and 2171, but not with strains that express K99, F41, or 987P adhesins. A cross-reaction was observed with 2 wild-type K88 strains, but not with a K12 strain that expresses K88 pili. Indirect immunofluorescence with these absorbed antisera revealed adherent bacteria in frozen sections of ileum from pigs infected with either strain. We concluded that these strains are pathogenic and express a common surface antigen that may be a novel adhesin in E coli strains that cause diarrhea in weaned pigs. 相似文献
2.
Sarah L Ralston 《Veterinary Clinics of North America: Equine Practice》2002,18(2):295-304, vii
Abnormally high or low blood glucose and insulin concentrations after standardized glucose tolerance tests can reflect disorders such as pituitary dysfunction, polysaccharide storage myopathies, and other clinical disorders. Glucose and insulin responses, however, are modified by the diet to which the animal has adapted, time since it was last fed, and what it was fed. Body fat (obesity), fitness level, physiologic status, and stress also alter glucose and insulin metabolism. Therefore, it is important to consider these factors when evaluating glucose and insulin tests, especially if only one sample it taken. This article describes the factors affecting glucose and insulin metabolism in horses and how they might influence the interpretation of standardized tests of glucose tolerance. 相似文献
3.
优化蛋白质营养不再仅仅是对日粮进行大致的氨基酸平衡。蛋白质的形式对猪的健康和生长会产生长远的影响。 蛋白质摄入量是生长猪营养中的一个基本要素。要提高瘦肉生长率,就需要平衡良好的氨基酸以供蛋白质合成之用,这些氨基酸需要以一定的形式通过日粮来提供。现在能够对饲料进行精确的分析,并且有来源可靠的合成氨基酸,已经为猪的蛋白质营养提供了坚实的基础。然而,有时候这会导致过量氮的排泄,因为并非提供的所有的蛋白质都会被猪吸收。过量氮的排出,不但会造成浪费,而且还会对环境造成污染。近年来,新的证据表明,单独提供氨基酸可能… 相似文献
4.
Major antigenic groups of rabies virus in Canada determined by anti-nucleocapsid monoclonal antibodies 总被引:5,自引:0,他引:5
W A Webster G A Casey K M Charlton 《Comparative immunology, microbiology and infectious diseases》1986,9(1):59-69
A total of 123 rabies virus isolates from various geographical areas in Canada were characterized by a panel of 43 anti-nucleocapsid monoclonal antibodies. Four major antigenic groups are found in terrestrial mammals: "Canadian Arctic" from Ontario, Quebec and the Northwest Territories; "south-eastern Georgian Bay" from Ontario; "south mid-central skunk" from Alberta, Saskatchewan and Manitoba; and "Brook's, Alberta skunk" from a restricted area in Alberta. Bat isolates can be divided into 4 major antigenic groups: "B-1" in Eptesicus fuscus from Ontario; "B-2" in a variety of bat species from British Columbia eastward into Ontario; "B-3" in Myotis spp. from Ontario and New Brunswick; and "B-4" in E. fuscus from Alberta and Saskatchewan. A single case of bat to horse transmission of rabies virus is recorded. These street isolates are compared with isolates of fixed virus. Epidemiological aspects are discussed. 相似文献
5.
6.
Kim M. Plummer Sarah J. Clark Lana M. Ellis Ashwini Loganathan Taha H. Al-Samarrai Erik H.A. Rikkerink Patrick A. Sullivan Matthew D. Templeton Peter C. Farley 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(3):265-274
Peptidases have been implicated in the pathogenicity of fungi that cause disease in plants. Expression of the secreted aspartic peptidase gene (gcsap), of a Glomerella cingulata isolate pathogenic on apples, is induced during appressorium formation. To determine whether the secreted aspartic peptidase (GcSAP) is essential to pathogenicity, gcsap was disrupted using a vector containing a 637 bp fragment of genomic DNA that encodes the sequence spanning the two active site aspartic acid (Asp) residues. To ensure that the truncated gcsap gene products could not have residual peptidase activity the codons for the active site residues Asp112 and Asp297 were both mutated to histidine residues. Both PCR and Southern analysis confirmed disruption of gcsap. Neither gcsap mRNA nor GcSAP activity was detected in the disruption mutant. Pathogenicity tests on fruit from three apple cultivars showed that GcSAP was not required for pathogenicity. The disruption mutant grew on medium containing protein as the sole source of nitrogen because G. cingulata secretes a previously undetected peptidase(s). A serine peptidase that had a pH optimum between pH 7.0 and 8.0 and a K
m of 0.25 mM for the synthetic substrate succinyl-Ala–Ala–Pro–Phe-p-nitroanilide was identified. 相似文献
7.
8.
Paixão Sonália Ferreira Fritzen Juliana Torres Tomazi Crespo Sarah Elizabeth Izzo de Moraes Pereira Helder Alfieri Alice Fernandes Alfieri Amauri Alcindo 《Tropical animal health and production》2018,50(8):1947-1950
Tropical Animal Health and Production - Serological studies have characterized the presence of the bovine viral diarrhea virus (BVDV) infection in water buffalo herds worldwide. However, the... 相似文献
9.
Effects of hyperbaric oxygen therapy on uncomplicated incisional and open wound healing in dogs
下载免费PDF全文
![点击此处可从《Veterinary surgery : VS》网站下载免费的PDF全文](/ch/ext_images/free.gif)
10.