A review on breeding and genetic strategies in Iranian buffaloes (<Emphasis Type="Italic">Bubalus bubalis</Emphasis>)

The aim of current study was to review breeding progress and update information on genetic strategies in Iranian buffaloes. Iranian buffalo is one of the vital domestic animals throughout north, north-west, south and south-west of Iran with measurable characteristics both in milk and meat production. The species plays an important role in rural economy of the country due to its unique characteristics such as resistance to diseases and parasites, having long productive lifespan and showing higher capability of consuming low-quality forage. In Iran, total production of milk and meat devoted to buffaloes are 293,000 and 24,700 tons, respectively. Selection activities and milk yield recording are carrying out by the central government through the Animal Breeding Centre of Iran. The main breeding activities of Iranian buffaloes included the estimation of genetic parameters and genetic trends for performance traits using different models and methods, estimation of economic values and selection criteria and analysis of population structure. Incorporating different aspects of dairy buffalo management together with improved housing, nutrition, breeding and milking, is known to produce significant improvements in buffalo production. Therefore, identifying genetic potential of Iranian buffaloes, selection of superior breeds, improving nutritional management and reproduction and developing the education and increasing the skills of practical breeders can be useful in order to enhance the performance and profitability of Iranian buffaloes.     

Herbaceous species diversity in relation to fire severity in Zagros oak forests,Iran

Zagros forests are mainly covered byQuercus brantii L. coppices and oak sprout clumps occupy the forest area like patches. We investigated post-fire herbaceous diversity in the first growing season after fire. For this purpose neighboring burned and unburned areas were selected with the same plant species and ecological conditions. The data were collected from areas subjected to different fire severities. Overall 6 treatments were considered with respect to fire severity and the mi-crosites of inside and outside of oak sprout clumps including： unburned inside and outside of sprout clumps （Ni and No）, inside of sprout clumps that burned with high fire severity （H）, inside of sprout clumps that burned with moderate fire severity （M）, outside of sprout clumps that burned with low fire severity （OH and OM）. Different herbaceous com-position was observed in the unburned inside and outside of oak sprout clumps. The species diversity and richness were increased in treatments burned with low and moderate fire severity. However, in treatment burned with high fire severity （H）, herbaceous cover was reduced, even-ness was increased, and richness and diversity were not significantly changed. We concluded that besides the microsites conditions in forest, fire severity is an inseparable part of the ecological effect of fire on her-baceous composition.     

Effect of dietary GroBiotic®‐A supplementation as a prebiotic on the intestinal microflora,growth performance,haemato‐serological parameters,survival rate and body composition in juvenile beluga (Huso huso Linnaeus, 1754)

The objectives of the present study were to investigate of GroBiotic^®‐A (GBA) on growth, autochthonous intestinal microbiota and haemato‐serological parameters of beluga juvenile. A total of 180 fish (40.82 ± 5.81 g) were fed diets containing graded levels of GBA (0, 5, 10 and 20 g kg^?1 diet) for 8 weeks. No significantly differences in body composition, total viable aerobic bacteria, mean cell volume, mean cell haemoglobin, mean cell haemoglobin concentration, neutrophils, eosinophils, monocytes, albumin, glucose, triglyceride, cholesterol, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and lactate dehydrogenase were detected between treatment groups. The fish fed on 10 and 20 g kg^?1 GBA significantly showed higher lactic acid bacteria, final weight, body weight increases, weight gain and lower feed conversion ratio compared with the control and 5 g kg^?1 groups. The group fed 20 g kg^?1 GBA showed a highly significant difference in condition factor, survival rate, final length, total red and white blood cells, lymphocytes, haematocrit, haemoglobin, total protein, total immunoglobulin and lysozyme activity. The specific growth rate of the treatment groups was significantly elevated compared with the control groups. These results indicated that GBA at level 20 g kg^?1 improved growth, welfare and survival of beluga juvenile.     

Gastrointestinal and hepatic enzyme activities in juvenile silvery-black porgy (<Emphasis Type="Italic">Sparidentex hasta</Emphasis>) fed essential amino acid-deficient diets

As amino acids (AAs) are vital molecules in the metabolism of all living organisms and are the building blocks of enzymes, a 6-week feeding trial was conducted for determining the influence of dietary essential amino acid (EAA) deficiencies on pancreatic, plasma, and hepatic enzyme activities in silvery-black porgy (initial weight 4.7?±?0.01 g) juveniles. Eleven isoproteic (ca. 47%) and isoenergetic (ca. 20.5 MJ kg^?1) diets were formulated including a control diet, in which 60% of dietary nitrogen were provided by intact protein (fish meal, gelatin, and wheat meal) and 40% by crystalline AA. The other 10 diets were formulated by 40% reduction in each EAA from the control diet. At the end of the experiment, fish fed with threonine-deficient diet showed the lowest survival rate (P?<?0.05), whereas growth performance decreased in fish fed all EAA-deficient diets, although the reduction in body growth varied depending on the EAA considered. Pancreatic enzymes (trypsin, lipase, α-amylase, and carboxypeptidase A) activities significantly decreased in fish fed the EAA-deficient diets in comparison with fish fed the control diet (P?<?0.05). Fish fed with the arginine-deficient diet had the highest plasma and liver alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase levels (P?<?0.05). Plasma and liver lactate dehydrogenase and superoxide dismutase showed the highest and lowest values, respectively, in fish fed the arginine and lysine-deficient diets (P?<?0.05). Plasma metabolites were significantly affected by dietary EAA deficiencies (P?<?0.05). The results of this study suggesting dietary EAA deficiencies led to reduction in growth performance as well as pancreatic and liver malfunction. Furthermore, arginine and lysine are the most limited EAA for digestive enzyme activities and liver health in silvery-black porgy.     

Effects of dietary protein and essential amino acid deficiencies on growth,body composition,and digestive enzyme activities of silvery-black porgy (Sparidentex hasta)

International Aquatic Research - A 6-week feeding trial was conducted to evaluate the effects of protein-free (PF) and essential amino-acid-deficient (EAAD) diets on the growth performance and...     

Effect of in vitro selected synbiotics (galactooligosaccharide and mannanoligosaccharide with or without Enterococcus faecalis) on growth performance,immune responses and intestinal microbiota of juvenile narrow clawed crayfish,Astacus leptodactylus leptodactylus Eschscholtz, 1823

The aim of this study was to determine the best synbiotic combination (based on growth and short‐chain fatty acids production) between Enterococcus faecalis and eight prebiotics. Based on the results of in vitro studies, E. faecalis + galactooligosaccharide (EGOS) and E. faecalis + mannanoligosaccharide (EMOS) were selected as synbiotics. A 126‐day feeding trial was conducted to compare the effects of prebiotics, probiotic and synbiotics on the growth indices, In vivo ADC of nutrients, digestive enzymes, hemolymph indices and finally, biological responses against 48‐hr Aeromonas hydrophila exposure challenges of juvenile (4.13 ± 0.12 g) crayfish. The highest values of SGR (2.19% body weight day^?1), VFI (2.75% body weight day^?1), survival rate (96.67%) and the lowest FCR (2.33) were observed in the juvenile crayfish fed the EGOS‐ diet. The significantly (p < .05) highest means of in vivo ADC_OM, in vivo ADC_CP, in vivo ADC_CF and in vivo ADC_GE were measured in crayfish fed the EGOS‐ diet. The mean survival rate of Aeromonas hydrophila‐injected crayfish fed the EGOS‐ diet (56%) was significantly (p < .05) higher than those of fed the control (8.67%) and other diets (22.67–35.32%). At the levels tested, 7.86 log CFU E. faecalis g^?1 + 10 g kg^?1 GOS in the diet was considered optimum.     

Effects of concentrate supplementation on carcass and meat quality attributes of feedlot finished Small East African goats

Effects of concentrate supplementation on carcass and meat quality of feedlot finished Small East African (SEA) goats were assessed using 23 animals (14.5 months old and 20.1 kg body weight). Goats were subjected to four levels of concentrate supplementation: ad libitum concentrate allowance (T100), 66% of ad libitum concentrate allowance (T66), 33% of ad libitum allowance (T33) and no concentrate (T0). All goats were slaughtered after 90 days of experimental period. The ad libitum concentrate intake attained by the goats was about 370 g DM/d. All concentrate-supplemented goats had similar (P > 0.05) total dry matter intake. T100 goats had 31 g and 14 g higher (P < 0.05) daily body weight gain than T33 and T66 goats, respectively. T100 and T66 goats were comparable in final live weight and empty body weight but both were heavier (P < 0.05) than that of T33 and T0 goats. Hot and cold carcass weights for both T100 and T66 goats were 3 kg heavier (P < 0.05) than that of T0 goats. Concentrate-supplemented goats had similar (P > 0.05) EUROP scores for carcass fatness. T100 and T66 goats had 6.5 and 3 units higher (P < 0.05) scores for conformation than T0 and T33 goats, respectively. Dressing percentage increased with levels of concentrate supplementation in a curvilinear fashion, with highest values in T66 goats. At 6 h post-mortem, muscle pH for concentrate-supplemented animals was significantly lower compared with T0 goats. Carcass fat content was 9% higher (P < 0.05) in concentrate-supplemented goats than in their contemporaries. No differences in cooking loss or shear force were observed among treatments, while these variables were affected by the type of muscle. It is concluded that feedlot finishing of SEA had limited effects on meat quality. Finishing SEA goats at 66% of their ad libitum concentrate intake, however, significantly improved weight gains and carcass fatness. Cost–benefit analyses are recommended before embarking on a large scale feedlot finishing of SEA goats.     

Digestibility of selected feedstuffs in subadult Caspian great sturgeon,Huso huso using settlement faecal collection and stripping methods

Apparent crude protein (ADC_CP), crude fat (ADC_CF) and gross energy (ADC_GE) digestibility coefficients of several typical and novel feedstuffs were determined to be employed in the formulated diets of subadult beluga (17.70 ± 0.234 kg) diets via two faecal collection methods of settlement (SFCM) and stripping (StM). The tested feedstuffs included three fish meals, three terrestrial animal by‐products, three plant protein concentrates, nine high‐protein plant meals and three low plant protein meals. ADC_CP values varied in different fish meals ranging from 89.5% for Mexican sardine meal to 93.2% for anchovy meal with SFCM. ADC_CP values for plant protein concentrates ranged from 82.8% for wheat gluten meal to 92% for soy protein with SFCM. ADC_CP ranged from 37.2% for whole wheat to 75.6% for spirulina meal with SFCM. ADC_GE was lower in low‐protein plant meals (64.1%) in comparison to high‐protein plant meals (70.2%). Fish meals showed the highest ADC_CF values. A significant (P < 0.05) quadratic regression relation (r² = 0.92) was observed among in vivo ADC_CP of five feedstuff classes fed to beluga and in vitro ADC_CP. The differences among ADC values appear to be significantly related to the effects of feedstuffs and therefore diet and faecal collection methods.     

Dietary supplementation effects of Pediococcus acidilactici as probiotic on growth performance,digestive enzyme activities and immunity response in zebrafish (Danio rerio)

This study was carried out to assess the effects of Pediococcus acidilactici on zebrafish (Danio rerio). Different levels of P. acidilactici including 0, 1 × 10⁶, 2 × 10⁶, 4 × 10⁶ and 8 × 10⁶ colony‐forming unit per g of the diet (cfug^?1) were examined in fish with 120 ± 10 mg weight for 60 days in a completely randomized design. The results showed that the best growth indices were recorded in group 4 × 10⁶ cfug^?1 (p < 0.05). The highest number of total viable count and lactic acid bacteria of intestine were found in group 4 × 10⁶ cfug^?1 (p < 0.05). The maximum activity of digestive enzymes including amylase, lipase, protease and alkaline phosphatase was observed in group 4 × 10⁶ cfug^?1. The highest activity for superoxide dismutase was recorded in group 4 × 10⁶ cfug^?1 while catalase, glutathione peroxidase and glutathione reductase showed the highest activity in group 8 × 10⁶ cfug^?1. The most growth inhibition zone of Aeromonas hydrophylla, Flavobacterium columnare, Vibrio anguillarum and Edwardsiella tarda was found in group 4 × 10⁶ cfug^‐1 (p < 0.05). Therefore, P. acidilactici as a probiotic improved growth and immunity of the zebrafish and could be used by zebrafish farmers.     

Growth,carcass yield and meat quality attributes of Red Maasai sheep fed wheat straw-based diets

Thirty-two castrated Red Maasai sheep (12.7 kg initial body weight, aged 12–18 months), were used in an 84-day experiment to evaluate diets based on treated straw upon growth performance, carcass yield and meat quality. The animals were blocked by weight into four similar groups and randomly allotted into four dietary treatments, with eight individually fed animals per treatment. The dietary treatments were ad libitum untreated wheat straw (UTS), wheat straw treated with urea and lime (TS), straw and ad libitum hay (UTSH), and TS and ad libitum hay (TSH). In addition, each experimental animal received 220 g/day (on as fed basis) of a concentrate diet. Treatment of straw increased (P < 0.05) dry matter intake (42.3 vs. 33.7 g/kg W ⁷⁵/day), energy intake (4.6 vs. 3.7 MJ ME/d) and the average daily gain (40.7 vs. 23.1 g). Animals on TS produced heavier (P < 0.05) carcasses (6.6 vs. 5.4 kg) with superior conformation than animals on UTS. Percentage cooking loss was higher in carcasses from animals fed TS compared to those from other diets. Except M. longissimus dorsi and M. semitendinosus, tenderness of muscles was not affected by diet but ageing of meat improved (P < 0.001) tenderness. Overall, straw treatment increased carcass yields with limited effects on meat quality attributes.