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1.
The ability of nine strains of avian infectious bronchitis virus (IBV) to induce chicken interferon has been investigated using Semliki Forest virus for the tests. The Beaudette, H120 and Connecticut 46 strains induced interferon in the allantoic fluids of embryonated hens' eggs, the highest titre (1 : 30) being associated with Beaudette; but these as well as the Massachusetts M-41 and H52 strains failed to yield interferon in primary monolayer cultures of chick kidney cells as did all nine strains in organ cultures of chick embryo trachea. None of six strains of IBV investigated was susceptible to the inhibitory effects of chicken interferon. 相似文献
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Holmes MA Townsend HG Kohler AK Hussey S Breathnach C Barnett C Holland R Lunn DP 《Veterinary immunology and immunopathology》2006,111(1-2):67-80
Horses are commonly vaccinated to protect against pathogens which are responsible for diseases which are endemic within the general horse population, such as equine influenza virus (EIV) and equine herpesvirus-1 (EHV-1), and against a variety of diseases which are less common but which lead to greater morbidity and mortality, such as eastern equine encephalomyelitis virus (EEE) and tetanus. This study consisted of two trials which investigated the antigenicity of commercially available vaccines licensed in the USA to protect against EIV, EHV-1 respiratory disease, EHV-1 abortion, EEE and tetanus in horses. Trial I was conducted to compare serological responses to vaccines produced by three manufacturers against EIV, EHV-1 (respiratory disease), EEE, and tetanus given as multivalent preparations or as multiple vaccine courses. Trial II compared vaccines from two manufacturers licensed to protect against EHV-1 abortion, and measured EHV-1-specific interferon-gamma (IFN-gamma) mRNA production in addition to serological evidence of antigenicity. In Trial I significant differences were found between the antigenicity of different commercial vaccines that should be considered in product selection. It was difficult to identify vaccines that generate significant immune responses to respiratory viruses. The most dramatic differences in vaccine performance occurred in the case of the tetanus antigen. In Trial II both vaccines generated significant antibody responses and showed evidence of EHV-1-specific IFN-gamma mRNA responses. Overall there were wide variations in vaccine response, and the vaccines with the best responses were not produced by a single manufacturer. Differences in vaccine performance may have resulted from differences in antigen load and adjuvant formulation. 相似文献
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Holmes CB Thirumurthy H Padian NS Goosby EP 《Science (New York, N.Y.)》2010,330(6001):176-7; author reply 177-8
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Neurokinin receptors in recurrent airway obstruction: A comparative study of affected and unaffected horses 下载免费PDF全文
Changaram S. Venugopal Earnestine P. Holmes Sumanth Polikepahad Susan Laborde Michael Kearney Rustin M. Moore 《Canadian journal of veterinary research》2009,73(1):25-33
The purpose of the study was to compare in vitro airway responses to neurokinin A & B (NKA and NKB) and expression of NK-2 receptors in airways of horses affected and unaffected with recurrent airway obstruction (RAO). Neurokinin-A, an inflammatory mediator belonging to the tachykinin family of neuropeptides, causes bronchoconstriction by binding to NK-2 receptors. Neurokinin-B is a lesser-known neuropeptide that acts on NK-3 receptors. Horses were placed into RAO-affected and RAO-unaffected groups based on their history, clinical scoring, and pulmonary function testing. Lung tissue from each lobe was collected for immunohistochemical staining for NK-2 receptors. Cumulative concentration-response relationships were determined on bronchial rings (4-mm wide) collected and prepared from the right diaphragmatic lung lobe to graded concentrations (half log molar concentrations 10−7M to 10−4M) of NKA and NKB. The results showed that NKA caused significantly greater contraction than NKB in both groups. In RAO-affected horses, both agents produced significantly greater bronchial contractions than those in the RAO-unaffected horses. Immunohistochemical staining showed that the overall NK-2 receptor distribution was significantly increased in bronchial epithelium and smooth muscles of bronchi and pulmonary vessels of RAO-affected than RAO-unaffected horses. The findings indicate that NK-2 receptors are up-regulated in RAO, suggesting that NK-2 receptor antagonists may have some therapeutic value in controlling the progression of airway hyperreactivity in horses affected with RAO. 相似文献
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W. Holmes 《Grass and Forage Science》1962,17(1):30-40
Many studies in recent years have stressed that grassland provides the greater part of the feed requirements of ruminant livestock in temperate lands, that a large proportion of this is supplied as grazing (83, 122) and that in this form it is the cheapest source of feed for ruminants (25, 38, 40). Several authors have contrasted the production estimated to be available from pasture with the much lower proportion—about 50% (74, 116)—harvested by the animal. For these reasons the efficiency of grass utilization under grazing conditions has received intensive study in the past 20 years and many of the principles of grazing management have been elucidated. Consideration of grazing management involves a study of the needs of the animals to be catered for, the sequence of grass crops which may be grown over the season, including the influence of special-purpose pastures and of fertilizer treatment on the yield and seasonal distribution of production, and the effective conservation of surplus herbage. In this review, however, attention is concentrated on the problems concerned in grazing management for the dairy cow during the main growing period of the year. The object of grazing management may be defined as 'to ensure a large supply of nutritious grazing over the growing season at a low cost and to utilize it in such a manner that physical waste of herbage and inefficient utilization by the animal are minimized and the productive capacity of the sward is maintained'. It is a complex subject, involving many interrelated factors including botanical, animal and per-acre considerations. These are briefly outlined before the available experimental data in grazing management practices are considered. 相似文献