排序方式: 共有6条查询结果,搜索用时 15 毫秒
1
1.
Inokuma H Yoshizaki Y Matsumoto K Okuda M Onishi T Nakagome K Kosugi R Hirakawa M 《Veterinary parasitology》2004,121(3-4):341-346
A total of 80 free-roaming dogs on Okinawa Island, Japan, were examined for Babesia infection using the polymerase chain reaction (PCR) and sequence analysis. Of 80 samples, 12 were positive in a Babesia genus-specific PCR. Consequent species-specific PCR for B. canis and B. gibsoni revealed that 5 (6.3%) and 7 (8.8%) dogs were infected with B. canis and B. gibsoni, respectively. Sequence analysis of the PCR products revealed that the 18S rRNA gene sequence of B. canis detected from dogs in Okinawa was very close to B. canis vogeli with sequence similarity of 99.94%. 相似文献
2.
Milagros Virhuez MENDOZA Kenzo YONEMITSU Keita ISHIJIMA Yudai KURODA Kango TATEMOTO Yusuke INOUE Hiroshi SHIMODA Ryusei KUWATA Ai TAKANO Kazuo SUZUKI Ken MAEDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(7):992
In Japan, hepatitis E virus (HEV) causes hepatitis in humans through the consumption of raw or undercooked meat, including game meat. In the present study, nationwide surveillance of HEV infection among a total of 5,557 wild animals, including 15 species, was conducted in Japan. The prevalence of anti-HEV antibodies in wild boar was 12.4%, with higher positive rates in big boars (over 50 kg, 18.4%) than in small individuals (less than 30 kg, 5.3%). Furthermore, HEV RNA was more frequently detected in piglets than in older boars. Interestingly, the detection of HEV among wildlife by ELISA and RT-PCR suggested that HEV infection in Sika deer was a very rare event, and that there was no HEV infection among wild animals except for wild boar, Sika deer and Japanese monkeys. In conclusion, wild boar, especially piglets, are at high risk of HEV infection, while other wild animals showed less risk or no risk of HEV transmission. 相似文献
3.
Junko SUZUKI Yohei NISHIO Yuki KAMEO Yutaka TERADA Ryusei KUWATA Hiroshi SHIMODA Kazuo SUZUKI Ken MAEDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1457-1463
In 2007–2008, a canine distemper virus (CDV) epidemic occurred among wild animals in
Wakayama Prefecture, Japan, and many mammals, including the wild boar and deer, were
infected. In this study, CDV prevalence among wild animals was surveyed before and after
the epidemic. At first, an enzyme-linked immunosorbent assay (ELISA) with horseradish
peroxidase-conjugated protein A/G was established to detect CDV antibodies in many
mammalian species. This established ELISA was available for testing dogs, raccoons and
raccoon dogs as well as virus-neutralization test. Next, a serological survey of wild
mammalians was conducted, and it was indicated that many wild mammalians, particularly
raccoons, were infected with CDV during the epidemic, but few were infected before and
after the epidemic. On the other hand, many raccoon dogs died during the epidemic, but CDV
remained prevalent in the remaining population, and a small epidemic occurred in raccoon
dogs in 2012–2013. These results indicated that the epidemic of 2007–2008 may have been
intensified by transmission to raccoons. 相似文献
4.
Ayuka Iwakiri Hitomi Ota Norihisa Matsushita Ryusei Haraguchi Toshihiro Yamada Kenji Fukuda 《Forest Pathology》2023,53(2):e12805
Serpula himantioides is a widely distributed saprotrophic fungus that causes root and butt rot in various tree species. In Japan, butt rot associated with S. himantioides is observed in Chamaecyparis pisifera and Abies sachalinensis. Previous studies have shown that S. himantioides includes five phylogenetically defined cryptic lineages, but the placement of Japanese isolates remains unclear. To clarify the phylogenetic relationship between Japanese S. himantioides and the five known lineages, we conducted a phylogenetic analysis using three newly collected Japanese isolates along with 74 S. himantioides isolates, based on the internal transcribed spacer (ITS) region, the 28 S large subunit (LSU) rDNA, the beta-tubulin (tub) gene, and the heat stress protein (hsp) gene. The concatenated phylogenetic tree showed that Japanese isolates composed a clade with a high bootstrap value distinct from the known lineages, indicating the Japanese isolates present a new cryptic lineage of S. himantioides. 相似文献
5.
Inokuma H Fujii K Matsumoto K Okuda M Nakagome K Kosugi R Hirakawa M Onishi T 《Veterinary parasitology》2002,110(1-2):145-152
A free-roaming dog in Okinawa island showed Anaplasma (Ehrlichia) platys-like inclusions within the platelets of peripheral blood samples. The inclusions were positive in indirect fluorescence test with anti-A. phagocytophila serum. The platelet count of the dog was 170,000 microl(-1). The sequence analysis of the 16S rRNA, citrate synthase and heat shock protein genes of DNA from the infected platelets confirmed that the inclusions were A. platys. This is the first detection of A. platys inclusions in dogs in Japan. 相似文献
6.
Tomoaki NII Md Zahorul ISLAM Satoru KAKE Mitsuya SHIRAISHI Takashi TAKEUCHI Hidefumi KUWATA Atsushi MIYAMOTO Etsumori HARADA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(11):1499
Bovine lactoferrin (BLF) reportedly lowers blood pressure and induces vasorelaxation, but its effect on nitric oxide (NO) production has not been established. Accordingly, we aimed to determine whether BLF induces NO production in bovine aortic endothelial cells, and the effects of extracellular free magnesium (Mg) ion concentrations on this NO production. BLF induced NO production time-dependently. NO production was markedly inhibited by the NO synthase inhibitor, NG-nitro-L-arginine methyl ester, in an effect abolished by L-arginine, but not D-arginine. NO production was suppressed at low concentrations, and enhanced at high concentrations, of Mg ions in culture medium. These results suggest that BLF has an important role in hypotensive effects. Mg ions may affect BLF-induced NO production. 相似文献
1