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Shun TAKEO Daichi SATO Koji KIMURA Yasunori MONJI Takehito KUWAYAMA Ryoka KAWAHARA-MIKI Hisataka IWATA 《The Journal of reproduction and development》2014,60(2):92-99
The aim of the present study was to address the effect of resveratrol-mediated
upregulation of sirtuin 1 (SIRT1) during oocyte maturation on mitochondrial function, the
developmental ability of oocytes and on mechanisms responsible for blockage of polyspermic
fertilization. Oocytes collected from slaughterhouse-derived ovaries were cultured in
TCM-199 medium supplemented with 10% FCS and 0 or 20 µM resveratrol (Res). We examined the
effect of Res on SIRT1 expression in in vitro-matured oocytes (Exp 1);
fertilization and developmental ability (Exp 2); mitochondrial DNA copy number (Mt
number), ATP content and mitochondrial membrane potential in matured oocytes (Exp 3); and
the time required for proteinase to dissolve the zona pellucida following in
vitro fertilization (as a marker of zona pellucida hardening), as well as on
the distribution of cortical granules before and after fertilization (Exp 4). In Exp 1,
the 20 µM Res treatment upregulated protein expression of SIRT1 in oocytes. In Exp 2, Res
treatment improved the ratio of normal fertilization and the total cell number of
blastocysts. In Exp 3, Res treatment significantly increased the ATP content in matured
oocytes. Additionally, Res increased the overall Mt number and mitochondrial membrane
potential, but the effect was donor-dependent. In Exp 4, Res-induced zona hardening
improved the distribution and exocytosis of cortical granules after in
vitro fertilization. In conclusion, Res improved the quality of oocytes by
improving mitochondrial quantity and quality. In addition, Res added to the maturation
medium enhanced SIRT1 protein expression in oocytes and improved fertilization via
reinforcement of the mechanisms responsible for blockage of polyspermic fertilization. 相似文献
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Miyako SUGIYAMA Ryoka KAWAHARA-MIKI Hirosuke KAWANA Koumei SHIRASUNA Takehito KUWAYAMA Hisataka IWATA 《The Journal of reproduction and development》2015,61(4):251-259
Mitochondrial numbers increase during oocyte growth. In this study, we collected oocytes and granulosa cell complexes (OGCs) from early antral follicles (EAFs) of aged cows (> 120 months of age) and examined the effects of resveratrol on mitochondrial generation, degradation, and quality in oocytes grown in vitro. We also examined the effects of resveratrol on gene expression of the granulosa cells. Resveratrol (20 µM) enhanced the expression of SIRT1 and induced autophagy in both granulosa cells and oocytes derived from aged cows. Culturing the OGCs with resveratrol increased mitochondrial DNA copy numbers in oocytes grown in vitro. Furthermore, resveratrol increased the ATP content in oocytes and improved the developmental ability of the oocytes to the blastocyst stage. Gene expression profiles in granulosa cells, as evaluated by next-generation sequencing technology, revealed that resveratrol enhanced the expression of EIF2-related
genes but downregulated the expression of mammalian target of rapamycin (mTOR)-, inflammation-, and cholesterol homeostasis-related genes in granulosa cells. In conclusion, resveratrol affected both oocytes and granulosa cells derived from aged cows and improved the quality of oocytes grown in vitro through upregulation of mitochondrial biogenesis and degradation in growing oocytes and conditioning of granulosa cells. 相似文献
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