Factors affecting the field performance of an attracticide against the codling moth Cydia pomonella

Factors affecting the efficacy of an attracticide strategy for the control of the codling moth Cydia pomonella L (Lepidoptera: Tortricidae) were investigated using laboratory and field experiments. The sex-pheromone-based insect-control strategy utilises 100-microliters droplets of a sticky, paste-like formulation containing 1 mg g-1 (E,E)-8,10-dodecadien-1-ol (codlemone) as an attractant for male moths and 40 mg g-1 cyfluthrin, a contact insecticide, applied to branches in the upper parts of the tree crown. The longevity of the treatment under field conditions was assessed in the laboratory by biological testing of variously aged samples of the attracticide formulation which had been applied to the bark of apple trees growing in commercial orchards. Electroantennogram responses of male moth antennae were used to compare codlemone release from the attracticide after different lengths of environmental exposure. Changes in insecticidal efficacy of the same samples were assessed with reference to the speed of knockdown (KT50) and the mortalities after 48 h among populations of male moths confined in cages containing samples of fresh and field-aged formulations. Gradual declines in both the amount of pheromone released and insecticidal activity were observed over the 10-week period of the experiments. Various factors associated with the behaviour of codling moths in the field which might influence the attracticide strategy were also investigated. Although the vertical position of attracticide sources within apple trees had a strong influence on their attractiveness, their horizontal position had none. Results of field trials showed that efficacy of the attracticide depends on the population density of the pest. Under the conditions of the current study a density of three or more sources per tree (= 4500 sources per ha) was required to attain satisfactory levels of codling moth control.     

The effect of adipocyte and heart fatty acid-binding protein genes on intramuscular fat and backfat content in Meishan crossbred pigs

Effects of genetic variation in porcine adipocyte and heart fatty acid-binding protein genes, A-FABP and H-FABP, respectively, on intramuscular fat (IMF) content and backfat thickness (BFT) were examined in F2 crossbreds of Meishan and Western pigs. The involvement of each FABP gene in IMF accretion was studied to confirm previous results for Duroc pigs. The F2 crossbred pigs were genotyped for various markers including microsatellite sequences situated within both FABP genes. Linkage analysis assigned the A-FABP and H-FABP genes to marker intervals S0001-S0217 (20 cM) on SSC4 and Sw316-S0003 (16.6 cM) on SSC6, respectively, refining previous chromosomal assignments. Next, the role of both chromosome regions/genes on genetic variation in IMF content and BFT was studied by 1) screening SSC4 and SSC6 for QTL affecting both traits by performing a line-cross analysis and 2) estimation of the effect of individual A-FABP and H-FABP alleles on both traits. In the first analysis, suggestive and chromosome-wise significant evidence for a QTL affecting IMF was detected on SSC6. The H-FABP gene is a candidate gene for this effect because it resides within the large region containing this putative QTL. The second analysis showed a considerable but nonsignificant effect of H-FABP microsatellite alleles on IMF content. Suggestive evidence for a QTL affecting BFT was found on SSC6, but H-FABP was excluded as a candidate gene. In conclusion, present and previous results support involvement of H-FABP gene polymorphisms in IMF accretion independently from BFT in pigs. Therefore, implementation of these polymorphisms in marker-assisted selection to control IMF content independently from BFT may be considered. In contrast to previous findings for Duroc pigs, no evidence was found for an effect of the A-FABP gene on IMF or BFT in this population.     

Genetic parameters for EUROP carcass traits within different groups of cattle in Ireland

The first objective of this study was to test the ability of systems of weighing and classifying bovine carcasses used in commercial abattoirs in Ireland to provide information that can be used for the purposes of genetic evaluation of carcass weight, carcass fatness class, and carcass conformation class. Secondly, the study aimed to test whether genetic and phenotypic variances differed by breed of sire. Variance components for carcass traits were estimated for crosses between dairy cows and 8 breeds of sire commonly found in the Irish cattle population. These 8 breeds were Aberdeen Angus, Belgian Blue, Charolais, Friesian, Hereford, Holstein, Limousin, and Simmental. A multivariate animal model was used to estimate genetic parameters within the Holstein sire breed group. Univariate analyses were used to estimate variance components for the remaining 7 sire breed groups. Multivariate sire models were used to formally test differences in genetic variances in sire breed groups. Field data on 64,443 animals, which were slaughtered in commercial abattoirs between the ages of 300 and 875 d, were analyzed in 8 analyses. Carcass fat class and carcass conformation class were measured using the European Union beef carcass classification system (EUROP) scale. For all 3 traits, the sire breed group with the greatest genetic variance had a value of more than 8 times the sire breed group with least genetic variance. Heritabilities ranged from zero to moderate for carcass fatness class (0.00 to 0.40), from low to moderate for carcass conformation class (0.04 to 0.36), and from low to high for carcass weight (0.06 to 0.65). Carcass weight was the most heritable (0.26) of the 3 traits. Carcass conformation class and carcass fatness class were equally heritable (0.17). Genetic and phenotypic correlations were all positive in the Holstein sire breed group. The genetic correlations varied from 0.11 for the relationship between carcass weight and carcass fatness class to 0.44 for the relationship between carcass conformation class and carcass fatness class. Carcass weight and classification data collected in Irish abattoirs are useful for the purposes of genetic evaluation for beef traits of Irish cattle. There were significantly different variance components across the sire breed groups.     

Impacts of urbanization on Florida Key deer behavior and population dynamics

Rapid human population growth and urbanization have had a negative impact on species biodiversity. As competition for resources between man and wildlife continues, it is important to understand the effects of urbanization on species. Endangered Key deer (Odocoileus virginianus clavium) are endemic to the Florida Keys which have undergone rapid human population growth and development over the past 30 years. Our study objectives were to evaluate the impacts of urban development on Key deer habitat use, population dynamics, behavior, and body mass. We used data from two comprehensive studies on Key deer spanning 30 years to evaluate these changes. Our results suggest that Key deer have become more urbanized, using urban areas more today than they did 30 years ago. Contrary to our predictions, survival was higher for more urban deer than for less urban deer. Problems still exist with mortality factors heavily impacting some portions of the deer population including lower survival associated with less urban male deer. Analysis of Key deer body mass also was converse to our predictions as deer weights appear to have increased over time. Collectively, our results suggest that over the past 30 years Key deer have become more urbanized and that deer plasticity has allowed them to adapt and persist in an urbanizing environment. However, the future ability of Key deer to persist in an environment with continued urban development is unknown. At some threshold, urban development would become unsustainable and unlike other forms of habitat change or environmental disturbances, urban development is in most cases irreversible requiring careful planning in habitat conservation strategies.     

Determinants of annual fluxes of CO2 and N2O in long-term no-tillage and conventional tillage systems in northern France

The greenhouse gases CO₂ and N₂O emissions were quantified in a long-term experiment in northern France, in which no-till (NT) and conventional tillage (CT) had been differentiated during 32 years in plots under a maize–wheat rotation. Continuous CO₂ and periodical N₂O soil emission measurements were performed during two periods: under maize cultivation (April 2003–July 2003) and during the fallow period after wheat harvest (August 2003–March 2004). In order to document the dynamics and importance of these emissions, soil organic C and mineral N, residue decomposition, soil potential for CO₂ emission and climatic data were measured. CO₂ emissions were significantly larger in NT on 53% and in CT on 6% of the days. From April to July 2003 and from November 2003 to March 2004, the cumulated CO₂ emissions did not differ significantly between CT and NT. However, the cumulated CO₂ emissions from August to November 2003 were considerably larger for NT than for CT. Over the entire 331 days of measurement, CT and NT emitted 3160 ± 269 and 4064 ± 138 kg CO₂-C ha⁻¹, respectively. The differences in CO₂ emissions in the two tillage systems resulted from the soil climatic conditions and the amounts and location of crop residues and SOM. A large proportion of the CO₂ emissions in NT over the entire measurement period was probably due to the decomposition of old weathered residues. NT tended to emit more N₂O than CT over the entire measurement period. However differences were statistically significant in only half of the cases due to important variability. N₂O emissions were generally less than 5 g N ha⁻¹ day⁻¹, except for a few dates where emission increased up to 21 g N ha⁻¹ day⁻¹. These N₂O fluxes represented 0.80 ± 0.15 and 1.32 ± 0.52 kg N₂O-N ha⁻¹ year⁻¹ for CT and NT, respectively. Depending on the periods, a large part of the N₂O emissions occurred was probably induced by nitrification, since soil conditions were not favorable for denitrification. Finally, for the period of measurement after 32 years of tillage treatments, the NT system emitted more greenhouses gases (CO₂ and N₂O) to the atmosphere on an annual basis than the CT system.     

Characterization of lettuce (Lactuca sativa L.) grown with biopesticides and deltamethrin

The purpose of this study was to evaluate the physico-chemical characteristics and the post-harvest loss of mass of curly lettuce (Lactuca sativa L.) cv. Verônica that was grown in a protective environment with applications of biopesticides. The curly lettuce was treated by spraying with neem oil, cassava flour wastewater (manipueira), biofertiliser or the control treatment of deltamethrin. We determined the weight, circumference, dry matter, ash, crude protein, total phenols and minerals profile for the heads of lettuce. After harvesting, the lettuce was packed into opened plastic bags and kept at 5 °C for 5 days to evaluate its weight loss. No differences were observed among the treatments for the physico-chemical parameters. The lettuce that was sprayed with manipueira or biofertiliser had the greatest weight loss during storage. The achievement of similar yields with cheaper products suggests a benefit of using organic bioinsecticides.     

Comment on "Nonreciprocal light propagation in a silicon photonic circuit"

We show that the structure demonstrated by Feng et al. (Reports, 5 August 2011, p. 729) cannot enable optical isolation because it possesses a symmetric scattering matrix. Moreover, one cannot construct an optical isolator by incorporating this structure into any system as long as the system is linear and time-independent and is described by materials with a scalar dielectric function.     

Coupling hysteresis analysis with sediment and hydrological connectivity in three agricultural catchments in Navarre,Spain

Purpose

Rain storm events mobilise large proportions of fine sediments in catchment systems. Sediments from agricultural catchments are often adsorbed by nutrients, heavy metals and other (in)organic pollutants that may impact downstream environments. To mitigate erosion, sediment transport and associated pollutant transport, it is crucial to know the origin of the sediment that is found in the drainage system, and therefore, it is important to understand catchment sediment dynamics throughout the continuity of runoff events.

Materials and methods

To assess the impact of the state of a catchment on the transport of fine suspended sediment to catchment outlets, an algorithm has been developed which classifies rain storm events into simple (clockwise, counter-clockwise) and compound (figure-of-eight; complex) events. This algorithm is the first tool that uses all available discharge and suspended sediment data and analyses these data automatically. A total of 797 runoff events from three experimental watersheds in Navarre (Spain) were analysed with the help of long-term, high-resolution discharge and sediment data that was collected between 2000 and 2014.

Results and discussion

Morphological complexity and in-stream vegetation structures acted as disconnecting landscape features which caused storage of sediment along the transport cascade. The occurrence of sediment storage along transport paths was therefore responsible for clockwise hysteresis due to the availability of in-stream sediment which could cause the “first flush” affect. Conversely, the catchment with steeper channel gradients and a lower stream density showed much more counter-clockwise hysteresis due to better downstream and lateral surface hydrological connectivity. In this research, hydrological connectivity is defined as the actual and potential transfer paths in a catchment. The classification of event SSC-Q hysteresis provided a seasonal benchmark value to which catchment managers can compare runoff events in order to understand the origin and locations of suspended sediment in the catchment.

Conclusions

A new algorithm uses all available discharge and suspended sediment data to assess catchment sediment dynamics. From these analyses, the catchment connectivity can be assessed which is useful to develop catchment land management.

     

Solanum sect. petota in Guatemala; Taxonomy and genetic resources

There are five wild potato species in Guatemala:Solanum agrimonifolium,S. bulbocastanum,S. clarum,S. demissum, andS. morelliforme. We conducted a collecting expedition there from September 11 to November 5, 1995. The goals of the expedition were to gather field data for taxonomic studies of the five species of Guatemalan wild potatoes and to collect potato germplasm. Our 43 true seed collections nearly quadruple the available wild potato germplasm for Guatemala, provide germplasm from most previously known localities, and add new ones. We provide a systematic treatment of Guatemalan wild potatoes, geographic and logistical data for collecting wild potatoes in Guatemala, statistics on human population growth and deforestation to help explain decline of wild potato populations, recommend areas for future collecting, and suggest two areas as in-situ reserves for wild potatoes