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Chifumi Nagai Jean-Jacques Rakotomalala Riko Katahira Yeyun Li Katsuya Yamagata Hiroshi Ashihara 《Euphytica》2008,164(1):133-142
The GCAs are new tetraploid interspecific hybrids developed in Madagascar from Coffea eugenioides, C. canephora and C. arabica. Selected GCA having genotype UF1023 contained 0.37% DW caffeine and no detectable theobromine in green beans. Low caffeine
accumulation in GCA plants is due mainly to the low biosynthetic activity of purine alkaloids, possibly the extremely weak
N-methyltransferase reactions in caffeine biosynthesis. No significant catabolic activity of caffeine was found in GCA-UF1023,
in common with almost all coffee plants including C. arabica. 相似文献
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Kohei TORIKAI Kazuma SHIMIZU Hiroaki NAGATOMO Mariko KASAI Megumi KATO-ITOH Yuko KAMADA Ikue SHIBASAKI Hyojung JEON Riko KIKUCHI Sayaka WAKAYAMA Fabian SUCHY Hiromitsu NAKAUCHI Teruhiko WAKAYAMA Eiji MIZUTANI 《The Journal of reproduction and development》2023,69(1):48
We examined various methods to enhance the accessibility of intracytoplasmic sperm injection (ICSI) technology to more users by making the technique easier, more efficient, and practical. First, the methods for artificially removing the mouse sperm tail were evaluated. Trypsin treatment was found to efficiently remove the sperm tails. The resultant sperm cells had a lower oocyte activation capacity; however, the use of activated oocytes resulted in the same fecundity as that of fresh, untreated sperm. Pre-activated oocytes were more resistant to physical damage, showed higher survival rates, and required less time per injection. Testing this method in rats yielded similar results, although the oocyte activation method was different. Remarkably, this method resulted in higher birth rates of rat progeny than with conventional methods of rat ICSI. Our method thereby streamlines mouse and rat ICSI, making it more accessible to laboratories across many disciplines. 相似文献
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Nobuki YOSHIZAWA Michiko SHINOTO Akiho KATAYAMA Riko BEKKU Kenichi INATANI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(2):223
Peaton virus (PEAV) is a type of arthropod-borne virus (arbovirus) belonging to the genus Orthobunyavirus, much like Akabane virus and Aino virus. These arboviruses cause stillbirth and congenital malformations of fetuses in ruminants. In Japan, abnormal birth in bovine caused by PEAV were reported in Okinawa, Kyushu, and Chugoku regions, but it has never been reported in Shikoku region. The abnormal birth occurred in 2020 in Ehime Prefecture (Shikoku region) and suspected of being caused by PEAV from results of clinical signs, pathological findings, and virus neutralization test using PEAV. However, PEAV was not detected and isolated. This report describes the case of abnormal birth in bovine suspected of being caused by PEAV first occurred in Shikoku region, Japan. 相似文献
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